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151.
Mandrell D Truong L Jephson C Sarker MR Moore A Lang C Simonich MT Tanguay RL 《Journal of laboratory automation》2012,17(1):66-74
The potential of the developing zebrafish model for toxicology and drug discovery is limited by inefficient approaches to manipulating and chemically exposing zebrafish embryos-namely, manual placement of embryos into 96- or 384-well plates and exposure of embryos while still in the chorion, a barrier of poorly characterized permeability enclosing the developing embryo. We report the automated dechorionation of 1600 embryos at once at 4 h postfertilization (hpf) and placement of the dechorionated embryos into 96-well plates for exposure by 6 hpf. The process removed ≥95% of the embryos from their chorions with 2% embryo mortality by 24 hpf, and 2% of the embryos malformed at 120 hpf. The robotic embryo placement allocated 6-hpf embryos to 94.7% ± 4.2% of the wells in multiple 96-well trials. The rate of embryo mortality was 2.8% (43 of 1536) from robotic handling, the rate of missed wells was 1.2% (18 of 1536), and the frequency of multipicks was <0.1%. Embryo malformations observed at 24 hpf occurred nearly twice as frequently from robotic handling (16 of 864; 1.9%) as from manual pipetting (9 of 864; 1%). There was no statistical difference between the success of performing the embryo placement robotically or manually. 相似文献
152.
Analyzed the film Three Approaches to Psychotherapy (E. L. Shostrom, 1966), which depicts C. Rogers (client-centered therapy), A. Ellis (rational-emotive therapy), and F. S. Perls (Gestalt therapy) interviewing the same client, Gloria. Verbal exchanges between participants were analyzed, using T. S. Essig and R. L. Russell's (1988) categories for analyzing psychological relations between subjects and events in discourse. Predictable patterns in how therapists of different theoretical orientations represent subjectivity in discourse can be screened with these categories. Classification categories are appended. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
153.
In the first section of the article, we examine some recent criticisms of the connectionist enterprise: first, that connectionist models are fundamentally behaviorist in nature (and, therefore, non-cognitive), and second that connectionist models are fundamentally associationist in nature (and, therefore, cognitively weak). We argue that, for a limited class of connectionist models (feed-forward, pattern-associator models), the first criticism is unavoidable. With respect to the second criticism, we propose that connectionist modelsare fundamentally associationist but that this is appropriate for building models of human cognition. However, we do accept the point that there are cognitive capacities for which any purely associative model cannot provide a satisfactory account. The implication that we draw from is this is not that associationist models and mechanisms should be scrapped, but rather that they should be enhanced.In the next section of the article, we identify a set of connectionist approaches which are characterized by active symbols — recurrent circuits which are the basis of knowledge representation. We claim that such approaches avoid criticisms of behaviorism and are, in principle, capable of supporting full cognition. In the final section of the article, we speculate at some length about what we believe would be the characteristics of a fully realized active symbol system. This includes both potential problems and possible solutions (for example, mechanisms needed to control activity in a complex recurrent network) as well as the promise of such systems (in particular, the emergence of knowledge structures which would constitute genuine internal models). 相似文献
154.
"Mega"-analysis was developed by M. Carlson and N. Miller (see record 1987-31249-001) as an extension of traditional meta-analytic procedures for conducting integrative reviews of existing research literatures. One such mega-analysis was conducted by Carlson and Miller to synthesize the literature on the relation between negative mood states and helping. That analysis found no support for a theoretical account (negative state relief) that had been confirmed previously by using various experimental approaches. In an attempt to reconcile the discrepancy, the logic and methods used in Carlson and Miller's mega-analysis of the negative mood-helping literature were examined, and several serious problems were found. These problems are discussed, and data are presented to show that the results of that mega-analysis, and perhaps all mega-analyses, should not be viewed with confidence. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
155.
Kalinnik Natalia Kiesel Robert Rauber Thomas Richter Marcel Rünger Gudula 《The Journal of supercomputing》2021,77(4):3484-3515
The Journal of Supercomputing - Scientific application codes are often long-running time- and energy-consuming parallel codes, and the tuning of these methods towards the characteristics of a... 相似文献
156.
Chen G Deng Q Szymczak A Laramee RS Zhang E 《IEEE transactions on visualization and computer graphics》2012,18(5):767-782
Morse decomposition provides a numerically stable topological representation of vector fields that is crucial for their rigorous interpretation. However, Morse decomposition is not unique, and its granularity directly impacts its computational cost. In this paper, we propose an automatic refinement scheme to construct the Morse Connection Graph (MCG) of a given vector field in a hierarchical fashion. Our framework allows a Morse set to be refined through a local update of the flow combinatorialization graph, as well as the connection regions between Morse sets. The computation is fast because the most expensive computation is concentrated on a small portion of the domain. Furthermore, the present work allows the generation of a topologically consistent hierarchy of MCGs, which cannot be obtained using a global method. The classification of the extracted Morse sets is a crucial step for the construction of the MCG, for which the Poincare′ index is inadequate. We make use of an upper bound for the Conley index, provided by the Betti numbers of an index pair for a translation along the flow, to classify the Morse sets. This upper bound is sufficiently accurate for Morse set classification and provides supportive information for the automatic refinement process. An improved visualization technique for MCG is developed to incorporate the Conley indices. Finally, we apply the proposed techniques to a number of synthetic and realworld simulation data to demonstrate their utility. 相似文献
157.
Examined the 10 most cited articles published in the Psychological Bulletin over the past 4 decades. Seven of the 10 articles were methodologically oriented. A survey of the authors revealed that highly cited articles have an important message that goes beyond momentary fads, are broad in topic and intended audience, accessible, and well written. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
158.
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160.
Determination of temperatures associated with room fires provides a means of assessing an important aspect of fire hazard: the likelihood of the occurrence of flashover. Layer temperatures in excess of 600°C have been associated with the occurrence of flashover. A data correlation has previously been presented to estimate layer temperatures for fires burning in the center of rooms. For fires in corners and along walls, restricted entrainment results in higher layer temperatures than predicted by the previous correlation. Modification factors for the previous correlation are developed to extend its applicability to wall and corner burning geometries. The present analysis suggests that a fire in a corner may cause flashover with only half the heat release rate necessary for a fire burning in the center of a room.
Reference: Frederick Mowrer and Robert Williamson, Estimating Room Temperatures from Fires along Walls and in Corners,Fire Technology, Vol. 23, May 1987, pp. 133–145. 相似文献