Plant oil bioconversion into increase biological activity through lipases derived from wastes

The aim of this study was to show that abundant and inexpensive plant oils can be biotransformed to increase biological activity (antioxidant, antimicrobial and cytotoxicity) through hydrolysis reaction catalysed by lipases. We tested homemade and commercial lipases through the biotransformation of nine different plant oils in forty different combinations. First, the chemical composition of the samples was investigated. Thereafter, biological tests were conducted to assess the antioxidant and antimicrobial activity of the sampled biotransformation products, as well as analyzed their influence on the viability of healthy and cancer cells. Summarising, sunflower, corn and olive oils modified by orange waste-obtained lipases presented the most promising results, reaching up to 90% of antioxidant activity increase and significant growth inhibition of bacteria colonies belonging to genera Escherichia, Listeria, Staphylococcus, Pseudomonas and Salmonella. In addition, those compounds affected human oral squamous carcinoma cells. The bioconversion of plant oils through lipases improves their biological properties and might be an option for biotechnological application.     

Construction of baselines for VoIP traffic management on open MANs

In the last century, owing to the constant evolution of technologies telecommunication networks have become increasingly robust, being able to support multiple services. These services are part of the heterogeneous network traffic that can be carried through the Internet. Many of these services, including VoIP, are latency sensitive. In other words, this means that their quality depends directly on the network quality of service. Since users tend to become more sensitive with the instability and unavailability of the network, it is important to improve traffic management. A particular type of data that could be used to improve VoIP traffic management is the Internet Protocol Detail Record (IPDR). IPDRs are tickets created by all VoIP call attempts which contain a group of information related to the call history. Because of its full range of information, IPDRs can be used to create VoIP traffic baselines. This paper presents the development of baselines based on IPDRs to support VoIP traffic management in open‐access Metropolitan Area Networks (MAN). Copyright © 2013 John Wiley & Sons, Ltd.     

Algorithm transformation methods to reduce the overhead of software-based fault tolerance techniques

This paper introduces a framework that tackles the costs in area and energy consumed by methodologies like spatial or temporal redundancy with a different approach: given an algorithm, we find a transformation in which part of the computation involved is transformed into memory accesses. The precomputed data stored in memory can be protected then by applying traditional and well established ECC algorithms to provide fault tolerant hardware designs. At the same time, the transformation increases the performance of the system by reducing its execution time, which is then used by customized software-based fault tolerant techniques to protect the system without any degradation when compared to its original form. Application of this technique to key algorithms in a MP3 player, combined with a fault injection campaign, show that this approach increases fault tolerance up to 92%, without any performance degradation.     

Experimental analyses of the poly(vinyl chloride) foams' mechanical anisotropic behavior

Assessing a full set of mechanical properties is a rather complicate task in the case of foams, especially if material models must be calibrated with these results. Many issues, for example anisotropy and heterogeneity, influence the mechanical behavior. This article shows through experimental analyses how the microstructure affects different experimental setups and it also quantifies the degree of anisotropy of a poly(vinyl chloride) foam. Monotonic and cyclic experimental tests were carried out using standard compression specimens and non‐standard tensile specimens. Results are complemented and compared with the aid of a digital image correlation technique and scanning electron microscopy analyses. Mechanical properties (e.g., elastic and plastic Poisson's ratios) are evaluated for compression and tensile tests, for two different material directions (normal and in‐plane). The material is found to be transversely isotropic. Differences in the results of the mechanical properties can be as high as 100%, or even more depending on the technique used and the loading direction. Also, the experimental analyses show how the material's microstructure behavior, like the evolution of the herein identified “yield fronts” and a “spring back” phenomenon, can influence the phenomenological response and the failure mechanisms as well as the hardening curves. POLYM. ENG. SCI., 2012. © 2012 Society of Plastics Engineers     

An Analysis of Public Clouds Elasticity in the Execution of Scientific Applications: a Survey

Elasticity can be seen as the ability of a system to increase or decrease the computing resources allocated in a dynamic and on demand way. It is an important feature provided by cloud computing, that has been widely used in web applications and is also gaining attention in the scientific community. Considering the possibilities of using elasticity in this context, a question arises: “Are the available public cloud solutions suitable to provide elasticity to scientific applications?” To answer the question, in a first moment we present a survey on the use of cloud computing in scientific scenarios, providing an overview of the subject. Next, we describe the elasticity mechanisms offered by major public cloud providers and analyzes the limitations of the solutions in providing elasticity for scientific applications. As the main contribution of the article, we also present an analysis over some initiatives that are being developed to overcome the current challenges. In our opinion, current computational clouds are developing rapidly but have not yet reached the necessary maturity level to meet all scientific applications elasticity requirements. We expect that in the coming years the efforts being taken by numerous researchers in this area identify and address these challenges and lead to better and more mature technologies that will improve cloud computing practices.     

Distributed Control Strategy with Smith's Predictor in a Pilot-Scale Diabatic Distillation Unit

Intrinsic characteristics of distillation such as dead time and high nonlinearities do not allow the complete elimination of transient times when any external disturbance or set-point change occurs. Thus, aiming at the use of easy-tuning systems, a distributed-action control in trays of a diabatic distillation unit with Smith's predictor was implemented in the Simulink environment to further reduce transient times and out-of-specification product. The distributed-action strategy with Smith's predictor led to a reduction of 33.3 min (33 %) in the transient time of the top temperature control loop and 66 % in out-of-specification product, when compared with the conventional strategy, and thus is shown to be an efficient approach to increasing the productivity of distillation plants.     

Effects of nisin concentration on properties of gelatin film-forming solutions and their films

The addition of nisin into a gelatin matrix can change properties of the film. The aim of this work was to develop gelatin-based films containing different nisin concentrations in order to study their influence on the film's antimicrobial and physical properties and their rheological properties as a film-forming solution (FFS). The FFS was characterised by rheological assays, and the gelatin-based active films were characterised and assessed by the effects of nisin concentrations on their various properties, including antimicrobial activity. Nisin's concentration affected not only its viscoelastic properties of FFS but also its film solubility in water, film surface roughness and light barrier. The addition of nisin also slightly modified the water contact angle and the mechanical properties of the gelatin films. Finally, the films demonstrated activity against Staphylococcus aureus and Listeria monocytogenes at concentrations above 56 mg of nisin g⁻¹ of gelatin.     

Design,Synthesis and Evaluation of 2,4-Diaminoquinazoline Derivatives as Potential Tubulin Polymerization Inhibitors

Microtubules are highly dynamic polymers composed of α- and β-tubulin proteins that have been shown to be potential therapeutic targets for the development of anticancer drugs. Currently, a wide variety of chemically diverse agents that bind to β-tubulin have been reported. Nocodazole (NZ) and colchicine (COL) are well-known tubulin-depolymerizing agents that have close binding sites in the β-tubulin. In this study, we designed and synthesized a set of nine 2,4-diaminoquinazoline derivatives that could occupy both NZ and COL binding sites. The synthesized compounds were evaluated for their antiproliferative activities against five cancer cell lines (PC-3, HCT-15, MCF-7, MDA-MB-231, and SK-LU-1), a noncancerous one (COS-7), and peripheral blood mononuclear cells (PBMC). The effect of compounds 4 e and 4 i on tubulin organization and polymerization was analyzed on the SK-LU-1 cell line by indirect immunofluorescence, western blotting, and tubulin polymerization assays. Our results demonstrated that both compounds exert their antiproliferative activity by inhibiting tubulin polymerization. Finally, a possible binding pose of 4 i in the NZ/COL binding site was determined by using molecular docking and molecular dynamics (MD) approaches. To our knowledge, this is the first report of non-N-substituted 2,4-diaminoquinazoline derivatives with the ability to inhibit tubulin polymerization.