Evaluation of damage in composites by using thermoelastic stress analysis: A promising technique to assess the stiffness degradation

The stiffness degradation represents one of the most interesting damage phenomena used for describing the fatigue behaviour of composites. A critical aspect of modelling the damage is represented by the simulation of the whole behaviour of the composite and by the assessment of the actual stiffness for the models validation. In this work, the stiffness degradation of quasi‐isotropic carbon fibre reinforced polymer (CFRP) obtained by automated fibre placement has been assessed by means of thermoelastic stress analysis. The amplitude of temperature signal at the mechanical frequency (thermoelastic signal) was considered as an indicator of material degradation and compared with the data provided by an extensometer. The correlation between thermoelastic and mechanical data allowed to build a new experimental model for evaluating and predicting material stiffness degradation by just using thermoelastic data. The proposed approach seems to be very promising for stiffness degradation assessment of real and complex mechanical components subjected to actual loading conditions.     

Potable water recovery from As, U, and F contaminated ground waters by direct contact membrane distillation process

In this study, the feasibility of the direct contact membrane distillation (DCMD) process to recover arsenic, uranium and fluoride contaminated saline ground waters was investigated. Two types of membranes (polypropylene, PP; and polytetrafluoroethylene, PTFE) were tested to compare the permeate production rates and contaminant removal efficiencies. Several experiments were conducted to study the effect of salts, arsenic, fluoride and uranium concentrations (synthetic brackish water with salts: 1000-10,000 ppm; arsenic and uranium: 10-400 ppb; fluoride: 1-30 ppm) on the desalination efficiency. The effect of process variables such as feed flow rate, feed temperature and pore size was studied. The experimental results proved that the DCMD process is able to achieve over 99% rejection of the salts, arsenic, fluoride and uranium contaminants and produced a high quality permeate suitable for many beneficial uses. The ability to utilize the low grade heat sources makes the DCMD process a viable option to recover potable water from a variety of impaired ground waters.     

LncRNA H19 Impairs Chemo and Radiotherapy in Tumorigenesis

Various treatments based on drug administration and radiotherapy have been devoted to preventing, palliating, and defeating cancer, showing high efficiency against the progression of this disease. Recently, in this process, malignant cells have been found which are capable of triggering specific molecular mechanisms against current treatments, with negative consequences in the prognosis of the disease. It is therefore fundamental to understand the underlying mechanisms, including the genes—and their signaling pathway regulators—involved in the process, in order to fight tumor cells. Long non-coding RNAs, H19 in particular, have been revealed as powerful protective factors in various types of cancer. However, they have also evidenced their oncogenic role in multiple carcinomas, enhancing tumor cell proliferation, migration, and invasion. In this review, we analyze the role of lncRNA H19 impairing chemo and radiotherapy in tumorigenesis, including breast cancer, lung adenocarcinoma, glioma, and colorectal carcinoma.     

Fenretinide in Cancer and Neurological Disease: A Two-Face Janus Molecule

Recently, several chemotherapeutic drugs have been repositioned in neurological diseases, based on common biological backgrounds and the inverse comorbidity between cancer and neurodegenerative diseases. Fenretinide (all-trans-N-(4-hydroxyphenyl) retinamide, 4-HPR) is a synthetic derivative of all-trans-retinoic acid initially proposed in anticancer therapy for its antitumor effects combined with limited toxicity. Subsequently, fenretinide has been proposed for other diseases, for which it was not intentionally designed for, due to its ability to influence different biological pathways, providing a broad spectrum of pharmacological effects. Here, we review the most relevant preclinical and clinical findings from fenretinide and discuss its therapeutic role towards cancer and neurological diseases, highlighting the hormetic behavior of this pleiotropic molecule.     

Improving Scalability of Application-Level Checkpoint-Recovery by Reducing Checkpoint Sizes

The execution times of large-scale parallel applications on nowadays multi/many-core systems are usually longer than the mean time between failures. Therefore, parallel applications must tolerate hardware failures to ensure that not all computation done is lost on machine failures. Checkpointing and rollback recovery is one of the most popular techniques to implement fault-tolerant applications. However, checkpointing parallel applications is expensive in terms of computing time, network utilization and storage resources. Thus, current checkpoint-recovery techniques should minimize these costs in order to be useful for large scale systems. In this paper three different and complementary techniques to reduce the size of the checkpoints generated by application-level checkpointing are proposed and implemented. Detailed experimental results obtained on a multicore cluster show the effectiveness of the proposed methods to reduce checkpointing cost.     

Combined ICA and FCA Schemes for a Hierarchical Network

A combined idle channel assignment (ICA) and fixed channel assignment (FCA) scheme is proposed to improve the traffic performance in a hierarchical network. This dual-mode network integrates the Frequency Division Duplex (FDD) and Time Division Duplex (TDD) modes of the Universal Mobile Telecommunication System (UMTS) and Terrestrial Radio Access (UTRA) in a given cell. This approach includes a high traffic load area and a blocked area as an example to evaluate the traffic performance. The ICA threshold and network timeout period effects on the traffic performance of this integrated dual-mode network are also investigated. The analytical results show that the handoff failure probabilities of the integrated dual-mode network can be reduced significantly with a minimal increase in the new call blocking probability when the combined ICA and FCA scheme replaces the FCA scheme. The integrated dual-mode network using the combined ICA and FCA scheme also increases the carried traffic. The traffic performance improvements for non-uniformly generated new calls are more significant than those for uniformly generated new calls when the combined ICA and FCA scheme is used. An increase in the high ICA threshold will result in an increase in the total carried traffic and an increase in the new call blocking and handoff failure probabilities for higher-tiered and low-tiered systems located in the high traffic load area. The traffic performance was evaluated using the discrete time simulation method to validate the analysis results.     

An architecture for a configurable mixed-signal device

The mixed-signal programmable system-on-chip (PSOC) architecture for high-volume low-cost applications is presented. Programmable analog, digital, and clocking circuits are combined with flash memory and a microcontroller to provide a platform for single-chip solutions for low-cost consumer applications. Both programmable analog and digital circuits are designed to support a moderate level of abstraction, balancing flexibility against cost and performance. A rough comparison of alternative approaches based on functionality and cost is presented.     

A Short ERAP2 That Binds IRAP Is Expressed in Macrophages Independently of Gene Variation

The M1 zinc metalloproteases ERAP1, ERAP2, and IRAP play a role in HLA-I antigen presentation by refining the peptidome either in the ER (ERAP1 and ERAP2) or in the endosomes (IRAP). They have also been entrusted with other, although less defined, functions such as the regulation of the angiotensin system and blood pressure. In humans, ERAP1 and IRAP are commonly expressed. ERAP2 instead has evolved under balancing selection that maintains two haplotypes, one of which undergoing RNA splicing leading to nonsense-mediated decay and loss of protein. Hence, likewise in rodents, wherein the ERAP2 gene is missing, about a quarter of the human population does not express ERAP2. We report here that macrophages, but not monocytes or other mononuclear blood cells, express and secrete an ERAP2 shorter form independent of the haplotype. The generation of this “short” ERAP2 is due to an autocatalytic cleavage within a distinctive structural motif and requires an acidic micro-environment. Remarkably, ERAP2 “short” binds IRAP and the two molecules are co-expressed in the endosomes as well as in the cell membrane. Of note, the same phenomenon could be observed in some cancer cells. These data prompt us to reconsider the role of ERAP2, which might have been maintained in humans due to fulfilling a relevant function in its “short” form.     

Fighting the Huntington’s Disease with a G-Quadruplex-Forming Aptamer Specifically Binding to Mutant Huntingtin Protein: Biophysical Characterization,In Vitro and In Vivo Studies

A set of guanine-rich aptamers able to preferentially recognize full-length huntingtin with an expanded polyglutamine tract has been recently identified, showing high efficacy in modulating the functions of the mutated protein in a variety of cell experiments. We here report a detailed biophysical characterization of the best aptamer in the series, named MS3, proved to adopt a stable, parallel G-quadruplex structure and show high nuclease resistance in serum. Confocal microscopy experiments on HeLa and SH-SY5Y cells, as models of non-neuronal and neuronal cells, respectively, showed a rapid, dose-dependent uptake of fluorescein-labelled MS3, demonstrating its effective internalization, even in the absence of transfecting agents, with no general cytotoxicity. Then, using a well-established Drosophila melanogaster model for Huntington’s disease, which expresses the mutated form of human huntingtin, a significant improvement in the motor neuronal function in flies fed with MS3 was observed, proving the in vivo efficacy of this aptamer.