Study of effects of fipronil and fipronil sulphone on meat nutritional quality and validation of confirmatory GC-MS/MS method for their analysis

The aim of this study was to validate the method for detection and quantification of fipronil and fipronil sulphone in meat products and study the effects of these pollutants on meat nutritional quality. The determination coefficients (R²) of the analytes were 0.999, while intra-laboratory CV% for repeatability and reproducibility ranged from 0.83% to 19.19%. Meat nutritional quality was assessed before and after the addition of analytes in meat samples. Both total and essential amino acids in meat decreased (P < 0.001) with increasing levels of added analytes, suggesting their detrimental effect on meat amino acid composition. The amino acid composition of meat did not show an univocal behaviour as consequence of fipronil and fipronil sulphone addition. In particular, aspartate, glutamate, arginine (P < 0.05), threonine, alanine, lysine and proline (P < 0.001) showed a significant decrease with increasing levels of added analytes, while serine (P < 0.001) evidenced an opposite trend.     

Nusinersen Modulates Proteomics Profiles of Cerebrospinal Fluid in Spinal Muscular Atrophy Type 1 Patients

Spinal muscular atrophy (SMA) type 1 is a severe infantile autosomal-recessive neuromuscular disorder caused by a survival motor neuron 1 gene (SMN1) mutation and characterized by progressive muscle weakness. Without supportive care, SMA type 1 is rapidly fatal. The antisense oligonucleotide nusinersen has recently improved the natural course of this disease. Here, we investigated, with a functional proteomic approach, cerebrospinal fluid (CSF) protein profiles from SMA type 1 patients who underwent nusinersen administration to clarify the biochemical response to the treatment and to monitor disease progression based on therapy. Six months after starting treatment (12 mg/5 mL × four doses of loading regimen administered at days 0, 14, 28, and 63), we observed a generalized reversion trend of the CSF protein pattern from our patient cohort to that of control donors. Notably, a marked up-regulation of apolipoprotein A1 and apolipoprotein E and a consistent variation in transthyretin proteoform occurrence were detected. Since these multifunctional proteins are critically active in biomolecular processes aberrant in SMA, i.e., synaptogenesis and neurite growth, neuronal survival and plasticity, inflammation, and oxidative stress control, their nusinersen induced modulation may support SMN improved-expression effects. Hence, these lipoproteins and transthyretin could represent valuable biomarkers to assess patient responsiveness and disease progression.     

The management of neuroendocrine tumours: A nutritional viewpoint

Nutritional status in patients with neuroendocrine tumours (NETs), especially of gastroenteropancreatic origin, can be deeply affected by excessive production of gastrointestinal hormones, peptides, and amines, which can lead to malabsorption, diarrhoea, steatorrhea, and altered gastrointestinal motility. Besides, the surgical and/or medical management of NETs can lead to alteration of gastrointestinal secretory, motor, and absorptive functions, with both dietary and nutritional consequences. Indeed, disease-related malnutrition is a frequently encountered yet both underrecognized and understudied clinical phenomenon in patients with NETs, with substantial prognostic and socioeconomic consequences. Most of these conditions can be alleviated by a tailored nutritional approach, also with the aim of improving the efficacy of cancer treatments. In this setting, skilled nutritionists can play a fundamental role in the multidisciplinary health care team in NETs management and their presence should be recommended. The aim of this review is to provide dietary advices for each specific condition in patients with NETs, underlining the importance of a nutritional approach to treat malnutrition in this setting. Further, we will provide preliminary evidence coming from our data on the assessment of nutritional status in a single cohort of patients with NETs.     

Self-anchoring and differentiation processes in the minimal group setting.

In-group favoritism in the minimal group setting was hypothesized to be a function of 2 processes: a tendency to base in-group judgments on the self (self-anchoring) and a tendency to assume 1 group to be the opposite of the other (differentiation). In the first 3 experiments, in which the order of rating the self and target group was varied, categorized and uncategorized participants were given trait information about 1 group and were asked to estimate the level of those traits in the other group. In-group judges tended to base group ratings on the self, whereas out-group and uncategorized judges inferred the 2 groups to be opposite of one another. Experiment 4 attempted to directly assess the direction of inference between self and in-group by giving feedback about self or in-group on unfamiliar dimensions and found that participants were more willing to generalize from self to in-group than from in-group to self. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

On the stability of the one-step exact collocation method for the second kind volterra integral equation with degenerate kernel

The local stability properties of the collocation method applied to a second kind Volterra integral equation with degenerate kernel are investigated. A finite length recurrence relation is derived and theorems for the local stability of the methods are proved.     

Assessing the contact‐activation of coagulation during hemodialysis with three different polysulfone filters: A prospective randomized cross‐over trial

Introduction: During hemodialysis (HD) the interaction of the blood with the dialyzer triggers both an inflammatory reaction and an activation of the coagulation cascade. An accepted parameter to quantify the extent of coagulation activation during HD is not available. This study aims to evaluate its amplitude, comparing dialyzers made of different polysulfone polymers, by measuring D‐dimers in the filter‐rinsing fluids (Frf) and to test whether Frf D‐dimers are suitable candidate markers to assess contact coagulation activation during HD. Methods: In a prospective, cross‐over study 41 hemodialysis patients were randomly allocated to nine HD sessions with three types of polysulfone membranes: Filter A: Poliflux®RevaclearMAX; Filter B: Helixone®Fx80, Filter C: Polyflux®H210. Findings: A total of 117 HD sessions were studied. The mean (SD) filter (Frf) D‐dimers were 0.19 µg/L (0.56) for Filter A; 0.66 µg/L (2.81) for Filter B; 0.33 µg/L (1.13) for Filter C. Significant differences were found: A vs. B (P < 0.01), A vs. C (P = 0.01); B vs. C not significant. A large between‐patient variability of D‐dimer filter levels was found. D‐Dimers in blood showed a similar trend but differences were not significant. Discussion: The contact activation of coagulation during HD may also vary among filters made up with similar polysulfones. D‐dimer in the filter rinsing fluid but not in the blood can be considered a candidate marker for the evaluation of thrombogenicity during HD. Further studies are needed to elucidate the mechanism(s) and to confirm the usefulness of filter rinsing fluid D‐Dimers as a clotting activation marker during HD.     

Cognitive dynamics of norm compliance. From norm adoption to flexible automated conformity

In this paper, an integrated, cognitive view of different mechanisms, reasons and pathways to norm compliance is presented. After a short introduction, theories of norm compliance are reviewed, and found to group in four main typologies: the rational choice model of norm compliance; theories based on conditional preferences to conformity, theories of thoughtless conformity, and theories of norm internalization. In the third section of the paper, the normative architecture EMIL-A is presented. Previous work discussed the epistemic module of this normative architecture, allowing for the generation of normative beliefs being formed. The fourth and fifth sections present the pragmatic modules of EMIL-A, i.e. norm adoption??leading to normative goals??and norm compliance??leading to their execution. Not only are several alternative reasons for norm adoption shown, but also several pathways to norm compliance are identified. Finally, a summary and ideas for future works conclude the paper.