Using electrophoretic deposition to identify protein charge in biological medium

Protein adsorption is the first step involved in establishing a suitable integration between a biomaterial and host tissue. It is therefore of highest interest to know the electric charge of proteins present in the relevant medium to be able to predict the behaviour of cells on given surfaces. In this study, electrophoretic deposition (EPD) was used as a simple method to identify the charge of proteins present in biological medium. In the model experiment carried out here, EPD was conducted using a biological medium containing 10% fetal calf serum (FCS) and the charge of the protein was determined by examining the migration of the protein in the EPD cell under a certain applied voltage. In addition, the suitability of EPD of proteins to deliver tailored surfaces for enhanced bioactivity or for controlled deposition of protein films on metallic surfaces was explored.     

Investigation into the Probable Cause of Failure of Economizer Tube of a Thermal Power Plant

In this investigation, we examined the probable cause of failure of a welded joint of a economizer tube of a 210 MW thermal power plant. X-ray radiography and chemical analysis along with detailed optical and scanning electron microscopic (SEM) examination of the economizer tube were carried out. It was concluded that severe blowholes, pipings, and porosity along with brittle fracture were responsible for the failure.     

Studying protein–ligand interactions using protein crystallography

Protein X-ray crystallography is now a relatively high-throughput technique that plays a major role in structure-based drug design programs where the method of soaking small organic ligands into protein crystals is used to confirm or identify binding modes. In this review, we discuss some experimental approaches for ‘soaking-in’ ligands into protein crystals where the major problem is poor solubility of the ligand. We also provide examples showing how crystal soaking can be used as a technique for establishing ligand binding strength: K _c (the apparent ligand–protein dissociation constant in the crystal), which for some proteins at least, is found to be very similar to the solution K _d. Kinetic effects are also found to be important and the rate at which ligands soak into crystals is shown to vary by orders of magnitude from 10^?1 to 10⁷?s depending on the system.     

A Note on Generalized Linear Phase Conditions for Causal FIR Systems

An alternative proof is presented for the necessary conditions for generalized linear phase in causal FIR systems, which appears to be somewhat crisper and more straightforward than the existing proofs available in the literature.     

Project Network Interdependency Alignment: New Approach to Assessing Project Effectiveness

The engineering and construction industry has evolved to a task-centric approach to evaluating the effectiveness of projects. However, a narrow task-based view of project network logic neglects the coordination of communication and knowledge exchanges across the project organizational network. This paper departs from traditional approaches to introduce a new approach to assessing project effectiveness that focuses on alignment of actual knowledge exchanges with knowledge exchange requirements across task-organization network dyads. A new modeling approach is introduced, called Project Network Interdependency Alignment. Project Network Interdependency Alignment identifies potentially excessive or insufficient communication and knowledge exchanges that can make projects ineffective. The modeling approach is introduced and retrospectively validated by using a building renovation construction project. The case study demonstrates that the approach can provide project managers with the capacity to analyze task and organizational network interdependence on projects and the critical capability to identify misalignments that impede project effectiveness.     

Understanding the use of tools for opening packaging

Various tools are available to improve the accessibility or ‘openability’ of packaging for those that may have potential difficulty. In this paper, the authors undertake an assessment of some of the common tools that have been designed to aid the aged in opening common packaging items with the aim of understanding their effectiveness. The study used a purpose built torque testing device embedded in a standard glass jar and asked participants to twist the lid of the device both unaided and with a tool and the maximum torque produced was noted. The study indicated that whilst some tools are effective, most however offer little or no benefit, as they do not overcome issues such as loss of dexterity and strength amongst the aged population.     

Nanoclay-modified asphalt materials: Preparation and characterization

The objective of this study is to review existing literature in the area of nano-modification of asphalt and proceed to apply nano-materials to asphalt to improve the performance. This study integrates literature review, preparation, and characterization of nano-modified asphalt materials. In the experimental testing montmorillonite, nanoclay at 2% and 4% by weight of asphalt was blended in asphalt binder at a high temperature to exfoliate the nanoclay within the asphalt. The asphalt binder was then characterized using the Superpave? rotational viscosity, dynamic shear modulus, and direct tension test. The rotational viscosity results indicate that the addition of the two types of nanoclay, Nanoclay A and Nanoclay B, increased the rotational viscosity by an average of 41% and 112%, respectively, across test temperatures 80, 100, 130, 135, 150 and 175 °C. It was found that the dynamic shear complex modulus (G*) value increases significantly across a range of testing temperatures (from 13 to 70 °C) and loading frequencies (0.01–25 Hz). With 2% Nanoclay A reinforcement in the asphalt binder, the complex shear moduli generally increased by 66% while the 4% Nanoclay A reinforcement in the asphalt binder generally increased the shear complex moduli by 125%. The 2% and 4% Nanoclay B increased the shear complex moduli by 184% and 196%, respectively. In terms of direct tension strength, the use of Nanoclay A and Nanoclay B reduced the strain failure rate of the original binder while the secant or direct tension moduli showed increase with the addition of the nanoclays. In furtherance of this research, nanoclay-modified asphalt is being tested at percentages higher than 4% to underscore the fact that nanoclays may have the potential to reduce rutting and cracking.     

EMG-based measures of fatigue during a repetitive squat exercise

We have demonstrated a technique to calculate the EMG instantaneous median frequency to assess muscle fatigue during a dynamic exercise commonly prescribed in patients with ACL deficiency. We used Cohen-Posch time-frequency representations to improve upon the variability of the instantaneous median frequency estimates derived using Cohen Class transformations. The technique was applied to surface EMG data recorded from the quadriceps and hamstring muscles of a control subject and a patient with ACL deficiency during a repetitive squat exercise. Instantaneous median frequency values were derived for the knee-extension phases of the exercise. Ensemble average and standard deviation of the instantaneous median frequency were computed for the portion of the cycle associated with the lowest variability of the mechanics     

A research strategy for investigating the ecological significance of endocrine disruption: report of a UK workshop.

In May 1997 the MRC Institute for Environment and Health hosted an expert, multidisciplinary workshop on the ecological effects of endocrine disruption, focusing on reproductive function, to identify research priorities. Particular objectives were to discuss the potential for effects at organisational levels higher than that of the individual (i.e. population, community and ecosystem levels), the range of taxa that may plausibly be susceptible, and our current knowledge of the environmental fate and behaviour of potential endocrine disrupters. The conclusions of this workshop are reported, with particular reference to a research strategy developed to ultimately address the significance of endocrine disruption at the level of the population. Specific research project areas considered to be of particular importance to the UK situation are also presented.     

Genetic sources of pheromone variation inColias eurytheme butterflies

The courtship pheromone ofColias eurytheme butterflies varies greatly among males in both the quantities and relative proportions of its three chemical components [n-heptacosane (C27), 13-methylheptacosane (13MH),n-nonacosane (C29)]. Narrow-sense heritabilities were high for the blend of 13MH and C27 and for the component quantities in one population (Kansas) but were low for the other population tested (Arizona). Genetic correlations between the three components were high in both populations, indicating a substantial degree of additive genetic influence on the component blends. High variability among populations in phenotypic correlations suggests that much of the male-to-male variation in the courtship pheromone may be attributable to environmental or developmental sources. Pheromone phenotypes do not seem to be associated with alba genotype.Lepidoptera: Pieridae.