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111.
Francesca Palombo C. Peter Winlove Ryan S. Edginton Ellen Green Nick Stone Silvia Caponi Marco Madami Daniele Fioretto 《Journal of the Royal Society Interface》2014,11(101)
Brillouin light scattering (BLS) spectroscopy is a technique that is able to detect thermally excited phonons within a material. The speed of propagation of these phonons can be determined from the magnitude of the Brillouin frequency shift between incident and scattered light, thereby providing a measure of the mechanical properties of the material in the gigahertz range. The mechanical properties of the extracellular matrices of biological tissues and their constituent biopolymers are important for normal tissue function and disturbances in these properties are widely implicated in disease. BLS offers the prospect of measuring mechanical properties on a microscopic scale in living tissues, thereby providing insights into structure–function relationships under normal and pathological conditions. In this study, we investigated BLS in collagen and elastin—the fibrous proteins of the extracellular matrix (ECM). Measurements were made on type I collagen in rat tail tendon, type II collagen in articular cartilage and nuchal ligament elastin. The dependence of the BLS spectrum on fibre orientation was investigated in a backscattering geometry using a reflective substrate. Two peaks, a bulk mode arising from phonon propagation along a quasi-radial direction to the fibre axis and a mode parallel to the surface, depending on sample orientation relative to the fibre axis, could be distinguished. The latter peak was fitted to a model of wave propagation through a hexagonally symmetric elastic solid, and the five components of the elasticity tensor were combined to give axial and transverse Young''s, shear and bulk moduli of the fibres. These were 10.2, 8.3, 3.2 and 10.9 GPa, and 6.1, 5.3, 1.9 and 8 GPa for dehydrated type I collagen and elastin, respectively. The former values are close to those previously reported. A microfocused BLS approach was also applied providing selection of single fibres. The moduli of collagen and elastin are much higher than those measured at lower frequency using macroscopic strains, and the difference between them is much less. We therefore believe, like previous investigators, that molecular-scale viscoelastic effects are responsible for the frequency dependence of the fibre biomechanics. Combining BLS with larger-scale mechanical testing methods therefore should, in the future, provide a means of following the evolution of mechanical properties in the formation of the complex structures found in the ECM. 相似文献
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Ryan Jakubowski Nathan Haws David Ellerbroek John Murtagh David Macfarlane 《Mine Water and the Environment》2014,33(2):133-145
An infiltration and salinity transport model (ISTM) based on the HYDRUS modeling platform was developed to support the beneficial use of treated coal seam gas (CSG) water to irrigate perennial forage plots in eastern Australia. The ISTM was used to support the expansion of irrigated areas under the CSG industry’s first specific irrigation beneficial use approval issued by the Queensland environmental regulatory administering authorities in March 2009. To support meeting regulatory requirements for expansion beyond the original irrigation area, forward modelling within the vadose zone was used to evaluate the fate and transport of the irrigation water as it migrates through the shallow soils and unsaturated bedrock toward the saturated zone; simulations beneath three agricultural systems with contrasting infiltration capacities were assessed. The model simulation results were used to guide irrigation activities to maximize water use in the readily available soil water capacity range for these agroecosystems, without detrimental impacts to plant growth or surface water/groundwater and soil resources. In this paper, a practical irrigation and rainfall systems-driven hydrogeochemical conceptual model is presented to identify processes that are likely to govern infiltration from the land surface, root water uptake, and solute transport into, and potentially through, the unsaturated zone. A numerical model is then parameterized to represent the primary processes hypothesized to affect water and salinity movement in the vadose zone. Finally, a series of simulations, conducted to identify the key parameters and processes governing the potential movement of water and salt through the root zone and into the bedrock vadose zone, are discussed. The simulations are used to support continuous improvement in modeling approaches for sustainably managing treated CSG extraction water allocated to irrigation. 相似文献
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Ryan S. Wong David F. Cechetto Shawn N. Whitehead 《International journal of molecular sciences》2016,17(9)
Alzheimer’s disease (AD) is the most common form of dementia, yet there are no therapeutic treatments that can either cure or delay its onset. Currently, the pathogenesis of AD is still uncertain, especially with respect to how the disease develops from a normal healthy brain. Amyloid β oligomers (AβO) are highly neurotoxic proteins and are considered potential initiators to the pathogenesis of AD. Rat brains were exposed to AβO via bilateral intracerebroventricular injections. Rats were then euthanized at either 1, 3, 7 or 21-days post surgery. Rat behavioural testing was performed using the Morris water maze and open field tests. Post-mortem brain tissue was immunolabelled for Aβ, microglia, and cholinergic neurons. Rats exposed to AβO showed deficits in spatial learning and anxiety-like behaviour. Acute positive staining for Aβ was only observed in the corpus callosum surrounding the lateral ventricles. AβO exposed rat brains also showed a delayed increase in activated microglia within the corpus callosum and a decreased number of cholinergic neurons within the basal forebrain. Acute exposure to AβO resulted in mild learning and memory impairments with co-concomitant white matter pathology within the corpus callosum and cholinergic cell loss within the basal forebrain. Results suggest that acute exposure to AβO in the rat may be a useful tool in assessing the early phases for the pathogenesis of AD. 相似文献
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A. F. Ghanem R. L. Williams M. H. Abdel Rehim Z. Ryan Tian 《Journal of Materials Science》2014,49(21):7382-7390
Nanoblending hydrophilic nanofillers thoroughly into hydrophobic polymer matrices has long been challenging, especially if involving no pre-functionalization on a 1D ceramic nanomaterial. Here we report a facile approach to fine-tuning of sodium titanate (Na2Ti3O7) nanobelt’s (NB) surface chemistry widely by exchanging the NB’s crystal lattice cations, for successfully nanoblending the low-cost and versatile NBs into the poly(vinyl benzyl chloride) or p(VBC) and the sulfonated form of pVBC’s [or sp(VBC)] matrixes. For the first time, the adjustable nanocompositing showed a long-sought workability in not only in situ radical polymerization of VBC monomer but also ex situ nanoblending of the p(VBC), with the NBs. The resultant nanocomposites possess an unusual surface versatility that can be tailored from being hydrophilic to being hydrophobic by design. This method concludes a generalized and industry-viable approach to mass-producing nanocomposites of many types facilely at low-cost, especially for large scale industries such as packaging materials. 相似文献
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Piloting the Smart Grid 总被引:1,自引:0,他引:1
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