Design of new inhibitors for cdc2 kinase based on a multiple pseudosubstrate structure

New inhibitors have been designed for cdc2 kinase based on a multiple pseudosubstrate structure. The new inhibitors have three different structural components: 3,4-bis(indol-3-yl)maleimide, Ac-Cys-(Ser)-Pro-Lys-Lys-NHMe, and ethyloxy group between the two components. Inhibitory activities toward cdc2 and other protein kinases were investigated, and the compound (21) with Ac-Cys-Pro-Lys-Lys-NHMe connected with the triethylene glycol spacer exhibited the most potent inhibition with relatively high selectivity.     

Evolution of nursing at University Hospitals: depositions by nurses who worked during the seventies

This study is part of a more extensive project that proposes to recover significant aspects related to nursing care evolution from 1950s until 1990s. This study is developed through the technique of oral declaration by active and retired registered nurses, in the context of an university-hospital from S?o Paulo State. The present study emphasizes the outcomes referring to the 1970s. As result becomes evident the undertaken effort by nurses in the struggle for profession's recognition and prestige; intense and deep transformations related to nurse's new roles as leadership of nursing staff and member of medical team.     

Dunham Treatment Including Effects of the Born-Oppenheimer Breakdown for Open-Shell Diatomic Molecules in 2Sigma States

Two high-performance liquid chromatographic (HPLC) methods are described for determination of (+/-)-ethopropazine (ET) in rat plasma. After deproteination and liquid-liquid extraction, assay of (+/-)-ET was performed using either a C18 column (non-stereospecific assay) or an (alpha-R-naphthyl)ethylurea column (stereospecific assay). The UV detection was at 250 nm. Mean recovery was >85%. Both assays demonstrated excellent linear relationships between peak height ratios and plasma concentrations; quantitation limits were < or =25 ng/ml, based on 100 microl rat plasma. Accuracy and precision were <17% with both methods. Both methods were applied successfully to the measurement of ET plasma concentrations in rats given the drug intravenously.     

A case of foramen magnum meningioma in which case enhanced three-dimensional CT scan was valuable for preoperative evaluation of the surgical approach

We report a case of foramen magnum meningioma in which case enhanced three-dimensional CT scan was valuable for preoperative evaluation of the surgical approach. A 53-year-old woman had suffered from stiffness and pain in the left occipital region and numbness of the left side of the face for about 2 years before admission. She had also weakness and numbness of the left side of her body for about 2 months before admission, and dysphagia and pain in the occipital region and in the posterior region of the neck produced by straining for about 1 month before admission. Neurological examination revealed left hemiparesis, and hypalgesia and tactile hypesthesia of the left side of the body, including the face. Plain X-P was normal. Enhanced CT scan and gadolinium enhanced MRI revealed a well-enhanced mass attached to the left anterolateral part of the foramen magnum. The left occipital condyle was observed at the lateral side of the attachment part of this mass. Angiography revealed tumor feeders from the meningeal branches of the left vertebral artery and the left ascending pharyngeal artery. Enhanced three-dimensional CT scan clearly showed that the tumor was attached to the left anterolateral part of the foramen magnum, that the left occipital condyle was at the lateral side of the attachment part of this mass and that the jugular foramen and jugular tubercle were situated superolateral to the attachment part of this mass. Considering these factors, we decided that removal of the posterior part of the left occipital condyle was necessary, but removal of the left jugular tubercle was not necessary for a good operative view from the left posterior lateral direction. The tumor was totally removed successfully and good results were obtained by the transcondylar approach without removal of the jugular tubercle. Histology of the tumor revealed meningothelial meningioma. In this case, preoperative evaluation with enhanced three-dimensional CT scan was helpful for deciding the surgical approach. With enhanced three-dimensional CT scan, it is easy to judge whether removal of the posterior part of the occipital condyle and/or the jugular tubercle is necessary for a good operative view, and we can get good images revealing the relationships between the tumor and surrounding structures. Preoperative evaluation with enhanced three-dimensional CT scan is very useful especially in this kind of situation.     

Long-term survivors with pN2 non-small cell lung cancer after a complete resection with a systematic mediastinal node dissection

OBJECTIVE: To determine the distribution and significance of microcalcifications in histologic sections of the prostate. DESIGN: Retrospective review of all histologic slides of completely embedded prostates from surgical specimens. MATERIALS: Randomly selected material included 266 radical prostatectomy and 10 cystoprostatectomy prostates without prostate cancer. Nonrandomly selected specimens included 26 radical prostatectomy specimens with a Gleason pattern 5 component, 24 cases with collagenous micronodules, and 8 cases previously noted to have microcalcifications within foci of prostate cancer. RESULTS: Four patterns of microcalcifications were noted in association with prostate cancer: (1) dystrophic calcification in the comedo-type necrosis of Gleason pattern 5, (2) intraluminal calcification in cribriform-type Gleason pattern 3 prostate cancer, (3) intraluminal calcification in small acinar adenocarcinoma, and (4) stromal calcification within collagenous micronodules associated with prostate cancer. Microcalcifications were noted in 32% of prostates without cancer; 1.9% of randomly selected prostates demonstrated microcalcifications associated with prostate cancer. CONCLUSIONS: Microcalcifications are less common in association with prostate cancer than with benign prostatic ducts and acini. However, intraluminal microcalcifications associated with an atypical small glandular proliferation should not be taken as unequivocal evidence of a benign process.     

Novel mode scrambler for reducing mode dependence in multimode optical waveguides

A novel mode scrambler for multimode optical waveguides is proposed to stabilise branching characteristics against the mode excitation conditio. A 2 × 2 optical star coupler is fabricated using this mode scrambler, and a stable branching ratio is realised with an excess insertion loss of 2.7 dB.     

Yagi-Uda array for millimetre-wave imaging

An imaging array consisting of five Yagi-Uda antennas in a dielectric has been constructed for 50 GHz that has antenna patterns suitable for millimetre-wave applications. Crosstalk levels of less than -30 dB between adjacent antennas in an f/1.0 imaging array have been measured in 5 GHz model experiments.<>     

Microstructure of carbonaceous mesophase spherule

Carbonaceous spherules of mesophase were examined by transmission electron microscopy (TEM) and X-ray diffraction to precisely clarify its structure. A series of thin sections were cut from a single spherule with a microtome, and the three-dimensional lamellar structure of the spherule investigated. From 002 dark-field images and selected area electron diffractions (SAD), it was observed that the spherule was of the Brooks-Taylor type, with the lamellae being parallel even at the poles of the spherule. Structural changes within the spherule was studied following carbonization and graphitization. The structure of the resultant coke was essentially that of spherule of mesophase. Size of the lamellae increased as well as. The number of stacking lamellae also increased, but a rapid increase was not seen. The spherules of mesophase show a high degree of homogeneity and an excellent degree of graphitization.