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21.
Lenka Monincová Dr. Milos Buděšínský Sabina Čujová Dr. Václav Čeřovský Dr. Václav Veverka 《Chembiochem : a European journal of chemical biology》2014,15(2):301-308
Lasiocepsin is a unique 27‐residue antimicrobial peptide, isolated from Lasioglossum laticeps (wild bee) venom, with substantial antibacterial and antifungal activity. It adopts a welldefined structure consisting of two α‐helices linked by a structured loop. Its basic residues form two distinct positively charged regions on the surface whereas aliphatic side chains contribute to solvent‐accessible hydrophobic areas, thus emphasising the amphipathic character of the molecule. Lasiocepsin structurally belongs to the ShK family and shows a strong preference for anionic phospholipids; this is further augmented by increasing concentrations of cardiolipin, such as those found at the poles of bacterial cells. The membrane‐permeabilising activity of the peptide is not limited to outer membranes of Gram‐negative bacteria. The peptide interacts with phospholipids initially through its N terminus, and its degree of penetration is strongly dependent on the presence of cardiolipin. 相似文献
22.
Reinaldo Rodríguez-Ramos P. Yan Juan C. López-Realpozo Raúl Guinovart-Díaz Julián Bravo-Castillero Federico J. Sabina C.P. Jiang 《Composite Structures》2011
In this work, the effective elastic moduli of two-phase fibrous periodic composites are obtained by means of the Asymptotic Homogenization Method (AHM) and eigenfunction expansion-variational method (EEVM), for different types of parallelogram cells. The constituents exhibit transversely isotropic properties. A doubly periodic parallelogram array of cylindrical inclusions under longitudinal shear is considered. The behavior of the shear elastic coefficient for different geometry arrays of the cell related to the angle of the fibers is studied. Some numerical examples and comparisons with other theoretical results demonstrate that both methods (AHM and EEVM) are efficients for the analysis of composites with presence of rhombic cell. The effect of the configuration of the cells on the shear effective property is observed. 相似文献
23.
A solid state reaction method was used to synthesize barium titanate (BT) and barium cerium titanate (BCT) ceramics at sintering temperature of 1473 K for 4 h. The effect of cerium (Ce) on the structure, microstructure and dielectric properties of BCT was investigated. The scanning electron microscopy (SEM) investigations revealed that the grain size increases with increasing Ce content. The X-ray diffraction (XRD) patterns showed mostly the BT phase, where the lattice parameter decreased with the addition of Ce. The temperature dependence of dielectric constant showed decrease in the phase transition temperature with higher Ce content. The dielectric constant decreased slightly with increasing frequency. The direct current (dc) density-voltage characteristics of the ceramics showed ohmic behavior for both the BT and BCT. As the temperature increased, the dc resistivity of the ceramics decreased. The activation energy increased with increasing Ce content. 相似文献
24.
Christian Monzio Compagnoni Alessandro S. Spinelli Andrea L. Lacaita Sabina Spiga 《Microelectronic Engineering》2006,83(10):1927-1930
We present a detailed experimental investigation of transient currents in HfO2 capacitors in the short timescale. We show that the transient currents flowing through the capacitor plates when the gate voltage is reset to zero after a low voltage stress period follow a power-law time dependence t−α (with α ? 1) over more than eight decades of time and down to the μs timescale. As transient currents in HfO2 are largely increased with respect to the SiO2 case, these results confirm that transient effects can be a severe issue for the successful integration of high-k dielectrics. 相似文献
25.
Schorr Gudrun; Ulbricht Sabina; Schmidt Carsten O.; Baumeister Sebastian E.; Rüge Jeannette; Schumann Anja; Rumpf Hans-Jürgen; John Ulrich; Meyer Christian 《Canadian Metallurgical Quarterly》2008,76(5):840
The authors examined the subtype structure of smokers classified in the precontemplation stage of change within the transtheoretical model. From a general practice-based sample of 1,499 daily smoking patients from Germany (participation rate 80%), they used a subgroup of 929 smokers who were classified in the precontemplation stage and applied latent class analysis, using the pros and cons of nonsmoking and smoking cessation self-efficacy as the defining variables. Cross-sectional validation of the emerging classes was based on smoking behavior and processes of change variables. For longitudinal validation, generalized estimation equation analyses were used on motivational and abstinence criteria from 6-, 12-, 18-, and 24-month follow-ups. A 4-class model best represented the data. Three subtypes (labeled progressive, immotive, and disengaged pessimistic) were similar to clusters identified in U.S. studies. The 4th (disengaged optimistic), by contrast, was reminiscent of a type that had previously only emerged in a Dutch study. Cross-sectional and longitudinal validation results confirmed the distinctiveness and predictive power of the classes. The findings highlight the importance of tailoring interventions for smoking behavior change to the needs of different subgroups of precontemplating smokers. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
26.
Gonzalo Allo Ayala 《TEST》1977,28(2-3):3-22
Sumario En la primera parte de este trabajo, se estudia la existencia de estrategias óptimas, en el modelo teórico (Pa F, ρ (Pa,f)) (ref. 3), generalización de un S-juego, dondeP
a es un conjunto de probabilidades finitamente aditivas, yF un subconjunto convexo de funciones reales acotadas. A continuación se analizan las clases de estrategias y se obtiene una
clase de estrategiasp
a-Bayes, más amplia que la de Bayes β, y Bayes extendida βo, que tiene la propiedad de contener siempre a la clase de estrategias admisibles. Se demuestra su completitud con hipótesis
adicionales, y se obtienen resultados relativos a estrategias σ-aditivas, como casos particulares.
Trabajo realizado con la beca March de Matemáticas, convocatoria 1973–74. 相似文献
27.
Phenolic compounds in olive oil and olive pomace from Cilento (Campania,Italy) and their antioxidant activity 总被引:3,自引:0,他引:3
Giuseppina Cioffi Maria Sabina Pesca Paolo De Caprariis Alessandra Braca Lorella Severino Nunziatina De Tommasi 《Food chemistry》2010
Virgin olive oil (VOO) has nutritional and sensory characteristics that make it unique and a basic component of the Mediterranean diet. Its importance is mainly attributed to its richness in polyphenols, which act as natural antioxidants and may contribute to the prevention of several human diseases. In this paper we report the determination and quantification of oleocanthal, one of the main substances responsible for the bitter taste of olive oil, together with a quali-quantitative analysis by HPLC analytical methods of phenolics from Cilento VOO and olive oil pomace. The total phenolic content was also determined and the in vitro antioxidant and free-radical scavenging activities by DPPH test was evaluated. A superoxide anion enzymatic assay was also carried out and the results were confirmed by the inhibition of xanthine oxidase activity assay. The possible protective role played by VOO secoiridoids on injurious effects of reactive oxygen metabolites on the intestinal epithelium, using Caco-2 human cell line, was investigated. 相似文献
28.
Bijlsma S Bobeldijk I Verheij ER Ramaker R Kochhar S Macdonald IA van Ommen B Smilde AK 《Analytical chemistry》2006,78(2):567-574
A large metabolomics study was performed on 600 plasma samples taken at four time points before and after a single intake of a high fat test meal by obese and lean subjects. All samples were analyzed by a liquid chromatography-mass spectrometry (LC-MS) lipidomic method for metabolic profiling. A pragmatic approach combining several well-established statistical methods was developed for processing this large data set in order to detect small differences in metabolic profiles in combination with a large biological variation. Such metabolomics studies require a careful analytical and statistical protocol. The strategy included data preprocessing, data analysis, and validation of statistical models. After several data preprocessing steps, partial least-squares discriminant analysis (PLS-DA) was used for finding biomarkers. To validate the found biomarkers statistically, the PLS-DA models were validated by means of a permutation test, biomarker models, and noninformative models. Univariate plots of potential biomarkers were used to obtain insight in up- or downregulation. The strategy proposed proved to be applicable for dealing with large-scale human metabolomics studies. 相似文献
29.
Galvano F Pietri A Bertuzzi T Gagliardi L Ciotti S Luisi S Bognanno M La Fauci L Iacopino AM Nigro F Li Volti G Vanella L Giammanco G Tina GL Gazzolo D 《Molecular nutrition & food research》2008,52(4):496-501
During 2006, 82 samples of human mature milk were collected at Italian hospitals and checked for aflatoxin M1 (AFM1) and ochratoxin A (OTA) by immunoaffinity column extraction and HPLC. AFM1 was detected in four (5%) of milk samples (ranging from < 7 ng/L to 140 ng/L; mean level: 55.35 ng/L); OTA was detected in 61 (74%) of milk samples (ranging from < 5 ng/L to 405 ng/L; mean level: 30.43 ng/L. OTA levels were significantly higher (p less, not double equals 0.05) in milk of habitual consumers of bread, bakery products and cured pork meat. No other statistically significant differences were observed although habitual consumers of pasta (p = 0.059), cookies (p = 0.061) and juices (p = 0.063) had mean contamination values of OTA higher than the moderate consumer. The very few AFB1 positive samples did not allow statistical comparisons. The present study confirms that the occurrence of OTA in human milk is related to maternal dietary habits. The findings support the possibility of dietary recommendations to woman, during pregnancy and lactation, aimed to tentatively reduce the OTA contamination of human milk. 相似文献
30.
New forms of artificial intelligence on the one hand and the ubiquitous networking of “everything with everything” on the other hand characterize the fourth industrial revolution. This results in a changed understanding of human–machine interaction, in new models for production, in which man and machine together with virtual agents form hybrid teams. The empirical study “Socializing with robots” aims to gain insight especially into conditions of development and processes of hybrid human–machine teams. In the experiment, human–robot actions and interactions were closely observed in a virtual environment. Robots as partners differed in shape and behavior (reliable or faulty). Participants were instructed to achieve an objective that could only be achieved via close teamwork. This paper unites different aspects from core disciplines of social robotics and psychology contributing to anthropomorphization with the empirical insights of the experiment. It focuses on the psychological effects (e.g. reactions of different personality types) on anthropomorphization and mechanization, taking the inter- and transdisciplinary field of social robotics as a starting point. 相似文献