The Addressing Fragment of Mitogaligin: First Insights into Functional and Structural Properties

Mitogaligin is a mitochondrion‐targeting protein involved in cell death. The sequence of the protein is unrelated to that of any known pro‐ or antiapoptotic protein. Mitochondrial targeting is controlled by an internal sequence from residues 31 to 53, and although this sequence is essential and sufficient to provoke cell death, the precise mechanism of action at the mitochondrial membrane remains to be elucidated. Here, by focusing on the [31–53] fragment, we first assessed and confirmed its cell cytotoxicity by microinjection. Subsequently, with the aid of membrane models, we evaluated the impact of the membrane environment on the 3D structure of the peptide and on how the peptide is embedded and oriented within membranes. The fragment is well organized, even though it does not contain a canonical secondary structure, and adopts an interfacial location. Structural comparison with other membrane‐interacting Trp‐rich peptides demonstrated similarities with the antimicrobial peptide tritrpcidin.     

Ribbon-like Foldamers for Cellular Uptake and Drug Delivery

Different intracellular delivery systems of bioactive compounds have been developed, including cell-penetrating peptides. Although usually nontoxic and biocompatible, these vectors share some of the general drawbacks of peptides, notably low bioavailability and susceptibility to protease degradation, that limit their use. Herein, the conversion of short peptide sequences into poly-α-amino-γ-lactam foldamers that adopt a ribbon-like structure is investigated. This template is used to distribute critical cationic and/or hydrophobic groups on both sides of the backbone, leading to potent short, cell-permeable foldamers with a low positive-charge content. The lead compound showed dramatically improved protease resistance and was able to efficiently deliver a biologically relevant cargo inside cells. This study provided a simple strategy to convert short peptide sequences into efficient protease-resistant cell-penetrating foldamers.     

Vinyl Dibromides: Versatile Partners in Cross-Coupling Reactions

Once limited to the preparation of alkynes, the chemistry of 1,1—dibromoalkenes has clearly undergone a renaissance through the application of palladium and copper catalysis. Remarkably efficient, innovative, and reliable methods have been recently designed around this chemistry, which has found many applications for the preparation of sophisticated building blocks as well as natural and/or biologically active products. This review article highlights major developments in this area.     

天津市成都道新建村住宅小区

项目背景：本项目位于天津市著名的五大道之一的成都道．是现代城市中心地段的高档高层住宅小区和沿街商业建筑的综合发展项目。建筑单体及总平面设计采用现代风格，设计任务书对景观设计的要求是：设计风格是后现代的．手法和材料是现代的．尽量将传承地域文化的片断融合在设计中。     

Silicon Nanotweezers With Subnanometer Resolution for the Micromanipulation of Biomolecules

We describe electrostatically actuated silicon nanotweezers which are intended for the manipulation and characterization of filamentary molecules. The microelectromechanical system consists of a pair of opposing tips whose distance can be accurately adjusted by means of an integrated differential capacitive sensor. The fabrication process is based on silicon-on-insulator technology and combines KOH wet anisotropic etching and deep reactive ion etching of silicon to form sharp nanotips and high aspect ratio microstructures, respectively. In the designed prototype, the initial gap between the tips was around 20 mum. The device showed a maximum displacement of about 2.5 mum, and we could achieve a resolution better than 0.2 nm (in static mode). We measured a resonant frequency of 2.5 kHz and a quality factor (Q factor) of 50 in air. The instrument was used to perform static and dynamic mechanical manipulations on DNA molecules, and we could distinctly observe the viscoelastic behavior of DNA bundles from these experiments.     

Synthesis of feedforward networks in supremum error bound

The main result of this paper is a constructive proof of a formula for the upper bound of the approximation error in L/sub /spl infin// (supremum norm) of multidimensional functions by feedforward networks with one hidden layer of sigmoidal units and a linear output. This result is applied to formulate a new method of neural-network synthesis. The result can also be used to estimate complexity of the maximum-error network and/or to initialize that network's weights. An example of the network synthesis is given.     

New one-pot synthesis of Au and Ag nanoparticles using green rust reactive particle as a micro-reactor

A new, simple, and fast one-pot synthesis of supported Au or Ag nanoparticles is implemented, for which a reactive Fe(II)-bearing green rust inorganic particle is used as an individual micro-reactor acting as both the reducing agent and support for the resulting metal nanoparticles. The mechanism involves both the solid-state oxidation of the green rust support (sulfate or carbonate) and the reduction-precipitation of soluble metal precursor. The resulting nanohybrids have a platy inorganic part supporting about one to ten nanoparticles with sizes in the 20 to 120 nm range.     

Dynamic pH model for autotrophic growth of microalgae in photobioreactor: A tool for monitoring and control purposes

A dynamic model for the photoautotrophic growth of microalgae in photobioreactor that describes the main variables of the system and allows the precise prediction of the pH in the culture was proposed and validated. The dynamic behavior of the biological system was expressed through a multistate model in continuous‐time formulation, based on mass‐balance equations and local photosynthetic responses of the anisotropic medium, further associated with a set of algebraic equations that describes the thermodynamic properties of the ammonia—carbon dioxide—water ternary solute system. The global photoautotrophic growth model was validated on experimental data acquired from a torus reactor inoculated with Chlamydomonas reinhardtii cells. The model response was studied in simulation for all identified input variables (dilution rate, incident light intensity, temperature, and flow rates of input gases). © 2013 American Institute of Chemical Engineers AIChE J 60: 585–599, 2014     

Configuration of a genetic algorithm used to optimise fibre steering in composite laminates

In a previous study, a genetic algorithm (GA) was applied to define the optimum orientation of fibres in a unidirectional laminate in which the fibre orientations were allowed to vary continuously across the domain. The results were positive, but computation time was excessively long. The present study was undertaken to address this issue. The elements used by the GA (encoded representation, fitness criterion, operators affecting the population) were examined and optimised to reduce computation time. It was found that the use of a strain-based fitness criterion was better than use of a stress-based criterion, regardless of whether the load was above or below the elastic limit. A good balance of elitism and mixing was necessary in the GA parameters to obtain the fastest convergence. Automatic imaging of the GA output was achieved using the SAMCEF software package.     

Decentralising a service-oriented architecture

Service-oriented computing is becoming an increasingly popular paradigm for modelling and building distributed systems in open and heterogeneous environments. However, proposed service-oriented architectures are typically based on centralised components, such as service registries or service brokers, that introduce reliability, management, and performance issues. This paper describes an approach to fully decentralise a service-oriented architecture using a self-organising peer-to-peer network maintained by service providers and consumers. The design is based on a gradient peer-to-peer topology, which allows the system to replicate a service registry using a limited number of the most stable and best performing peers. The paper evaluates the proposed approach through extensive simulation experiments and shows that the decentralised registry and the underlying peer-to-peer infrastructure scale to a large number of peers and can successfully manage high peer churn rates.