Spatial distribution and habitat characterization of schistosomiasis host snails in lake and land habitats of western Kenya

Intermediate host snails of schistosomiasis were surveyed in this study to determine their abundance and distribution in the lake and land aquatic habitats of Lake Victoria basin of Kenya. Several sites were sampled at eight locations, both in the lake and on the land. The habitat and/or vegetation type (i.e. open water, hippo grass, hyacinth, ambatch trees, other vegetation, stream, swamp, pond, dam) of the sampled aquatic sites within the locations were also differentiated, water physicochemical parameters were determined, and the abundance of different species or taxa of phytoplankton and zooplankton were enumerated and correlated with the abundance of schistosomiasis snails in the sites. The results indicated significantly more Biomphalaria sudanica snails than Bulinus africanus snails in different physical habitats on land (Student's t‐test, P < 0.05), as well as in different locations on land (Student's t‐test, P = 0.026). Regression analyses revealed that several physicochemical parameters, including dissolved oxygen (R² = ?0.659; n = 8; P = 0.014), pH (R² = 0.728; n = 8; P = 0.007) and turbulence (R² = ?0.616; n = 8; P = 0.02), were predictive of Biomphalaria spp. abundance, while pH (R² = 0.610; n = 8; P = 0.02) and turbulence (R² = ?0.578; n = 8; P = 0.028) were predictive of Bulinus spp. abundance in different locations in the lake. Cyanobacteria (R² = 0.638; n = 8; P = 0.02) and chlorophyceae (R² = ?0.50; n = 8; P = 0.05) were shown to be predictive of both Biomphalaria spp. and Bulinus spp. abundance in different locations in the lake. Zooplankton abundance varied significantly between different locations in the lake (One‐way anova , P < 0.001). Bosmina spp. were found to be predictive of both Biomphalaria spp. (R² = ?0.627; n = 8; P = 0.01) and Bulinus spp. (R² = ?0.50; n = 8; P = 0.05) in different locations in the lake. The results from this study will help inform policy regarding control measures for schistosomias and intermediate snail hosts in Lake Victoria waters, as well as in adjacent terrestrial aquatic habitats and even beyond.     

Use of FRP for RC Frames in Seismic Zones: Part II. Performance of Steel-Free GFRP-Reinforced Beam-Column Joints

The use of FRP as reinforcement in concrete structures has been growing rapidly due to its advantages over conventional steel reinforcement (e.g., corrosion resistance, light weight, magnetic neutrality). A potential application of FRP reinforcement is in structural concrete frames. However, current seismic design standards and detailing criteria for beam-column joints were established for steel reinforcement and may be unsuitable for FRP reinforcement due to its different mechanical properties. During recent earthquakes, many structural collapses were initiated or caused by beam-column joint failures. Since there are no detailed specifications for the application of FRP reinforcement in seismic zones, research is needed to gain a better understanding of the behaviour of FRP-reinforced concrete under seismic loading. In this study, two full-scale beam-column joint specimens reinforced with steel and GFRP, respectively, were tested in order to investigate their performance in the event of an earthquake. The control steel-reinforced specimen is detailed according to the Canadian Code (CSA A23.3-94) recommendations. The GFRP-reinforced specimen is detailed in a similar scheme but using a GFRP grid. The behaviour of the two specimens under reversed cyclic loading, their load-storey drift envelope relationship and energy dissipation ability were compared. The GFRP-reinforced specimen showed a predominantly elastic behaviour up to failure. While its energy dissipation was low, its performance was acceptable in terms of total storey drift demand.     

New Beta-lactamases in Candidate Phyla Radiation: Owning Pleiotropic Enzymes Is a Smart Paradigm for Microorganisms with a Reduced Genome

The increased exploitation of microbial sequencing methods has shed light on the high diversity of new microorganisms named Candidate Phyla Radiation (CPR). CPR are mainly detected via 16S rRNA/metabarcoding analyses or metagenomics and are found to be abundant in all environments and present in different human microbiomes. These microbes, characterized by their symbiotic/epiparasitic lifestyle with bacteria, are directly exposed to competition with other microorganisms sharing the same ecological niche. Recently, a rich repertoire of enzymes with antibiotic resistance activity has been found in CPR genomes by using an in silico adapted screening strategy. This reservoir has shown a high prevalence of putative beta-lactamase-encoding genes. We expressed and purified five putative beta-lactamase sequences having the essential domains and functional motifs from class A and class B beta-lactamase. Their enzymatic activities were tested against various beta-lactam substrates using liquid chromatography-mass spectrometry (LC-MS) and showed some beta-lactamase activity even in the presence of a beta-lactamase inhibitor. In addition, ribonuclease activity was demonstrated against RNA that was not inhibited by sulbactam and EDTA. None of these proteins could degrade single- and double-stranded-DNA. This study is the first to express and test putative CPR beta-lactamase protein sequences in vitro. Our findings highlight that the reduced genomes of CPR members harbor sequences encoding for beta-lactamases known to be multifunction hydrolase enzymes.     

A low‐power,area‐efficient multichannel receiver for micro MRI

A new integrated, low‐noise, low‐power, and area‐efficient multichannel receiver for magnetic resonance imaging (MRI) is described. The proposed receiver presents an alternative technique to overcome the use of multiple receiver front‐ends in parallel MRI. The receiver consists of three main stages: low‐noise pre‐amplifier, quadrature down‐converter, and a band pass filter (BPF). These components are used to receive the nuclear magnetic resonance signals from a 3 × 3 array of micro coils. These signals are combined using frequency domain multiplexing (FDM) method in the pre‐amplifier and BPF stages, then amplified and filtered to remove any out‐of‐band noise before providing it to an analog‐to‐digital converter at the low intermediate frequency stage. The receiver is designed using a 90 nm CMOS technology to operate at the main B₀ magnetic field of 9.4 T, which corresponds to 400 MHz. The receiver has an input referred noise voltage of 1.1 nV/√Hz, a total voltage gain of 87 dB, a power consumption of 69 mA from a 1 V supply voltage, and an area of 305 µm × 530 µm including the reference current and bias voltage circuits. Copyright © 2013 John Wiley & Sons, Ltd.     

The Endothelium and COVID-19: An Increasingly Clear Link Brief Title: Endotheliopathy in COVID-19

The endothelium has a fundamental role in the cardiovascular complications of coronavirus disease 2019 (COVID-19). Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) particularly affects endothelial cells. The virus binds to the angiotensin-converting enzyme 2 (ACE-2) receptor (present on type 2 alveolar cells, bronchial epithelial cells, and endothelial cells), and induces a cytokine storm. The cytokines tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6 have particular effects on endothelial cells—leading to endothelial dysfunction, endothelial cell death, changes in tight junctions, and vascular hyperpermeability. Under normal conditions, apoptotic endothelial cells are removed into the bloodstream. During COVID-19, however, endothelial cells are detached more rapidly, and do not regenerate as effectively as usual. The loss of the endothelium on the luminal surface abolishes all of the vascular responses mediated by the endothelium and nitric oxide production in particular, which results in greater contractility. Moreover, circulating endothelial cells infected with SARS-CoV-2 act as vectors for viral dissemination by forming clusters that migrate into the circulation and reach distant organs. The cell clusters and the endothelial dysfunction might contribute to the various thromboembolic pathologies observed in COVID-19 by inducing the formation of intravascular microthrombi, as well as by triggering disseminated intravascular coagulation. Here, we review the contributions of endotheliopathy and endothelial-cell-derived extracellular vesicles to the pathogenesis of COVID-19, and discuss therapeutic strategies that target the endothelium in patients with COVID-19.     

Hybrid clustering analysis using improved krill herd algorithm

In this paper, a novel text clustering method, improved krill herd algorithm with a hybrid function, called MMKHA, is proposed as an efficient clustering way to obtain promising and precise results in this domain. Krill herd is a new swarm-based optimization algorithm that imitates the behavior of a group of live krill. The potential of this algorithm is high because it performs better than other optimization methods; it balances the process of exploration and exploitation by complementing the strength of local nearby searching and global wide-range searching. Text clustering is the process of grouping significant amounts of text documents into coherent clusters in which documents in the same cluster are relevant. For the purpose of the experiments, six versions are thoroughly investigated to determine the best version for solving the text clustering. Eight benchmark text datasets are used for the evaluation process available at the Laboratory of Computational Intelligence (LABIC). Seven evaluation measures are utilized to validate the proposed algorithms, namely, ASDC, accuracy, precision, recall, F-measure, purity, and entropy. The proposed algorithms are compared with the other successful algorithms published in the literature. The results proved that the proposed improved krill herd algorithm with hybrid function achieved almost all the best results for all datasets in comparison with the other comparative algorithms.     

Oxy‐fuel combustion technology: current status,applications, and trends

The increased level of emissions of carbon dioxide into the atmosphere due to burning of fossil fuels represents one of the main barriers toward the reduction of greenhouse gases and the control of global warming. In the last decades, the use of renewable and clean sources of energies such as solar and wind energies has been increased extensively. However, due to the tremendously increasing world energy demand, fossil fuels would continue in use for decades which necessitates the integration of carbon capture technologies (CCTs) in power plants. These technologies include oxycombustion, pre‐combustion, and post‐combustion carbon capture. Oxycombustion technology is one of the most promising carbon capture technologies as it can be applied with slight modifications to existing power plants or to new power plants. In this technology, fuel is burned using an oxidizer mixture of pure oxygen plus recycled exhaust gases (consists mainly of CO₂). The oxycombustion process results in highly CO₂‐concentrated exhaust gases, which facilitates the capture process of CO₂ after H₂O condensation. The captured CO₂ can be used for industrial applications or can be sequestrated. The current work reviews the current status of oxycombustion technology and its applications in existing conventional combustion systems (including gas turbines and boilers) and novel oxygen transport reactors (OTRs). The review starts with an introduction to the available CCTs with emphasis on their different applications and limitations of use, followed by a review on oxycombustion applications in different combustion systems utilizing gaseous, liquid, and coal fuels. The current status and technology readiness level of oxycombustion technology is discussed. The novel application of oxycombustion technology in OTRs is analyzed in some details. The analyses of OTRs include oxygen permeation technique, fabrication of oxygen transport membranes (OTMs), calculation of oxygen permeation flux, and coupling between oxygen separation and oxycombustion of fuel within the same unit called OTR. The oxycombustion process inside OTR is analyzed considering coal and gaseous fuels. The future trends of oxycombustion technology are itemized and discussed in details in the present study including: (i) ITMs for syngas production; (ii) combustion utilizing liquid fuels in OTRs; (iii) oxy‐combustion integrated power plants and (iv) third generation technologies for CO₂ capture. Techno‐economic analysis of oxycombustion integrated systems is also discussed trying to assess the future prospects of this technology. Copyright © 2017 John Wiley & Sons, Ltd.     

Opportunities for plant-derived enhancers for iron,zinc, and calcium bioavailability: A review

Understanding of the mechanism of interactions between dietary elements, their salts, and complexing/binding ligands is vital to manage both deficiency and toxicity associated with essential element bioavailability. Numerous mineral ligands are found in both animal and plant foods and are known to exert bioactivity via element chelation resulting in modulation of antioxidant capacity or micobiome metabolism among other physiological outcomes. However, little is explored in the context of dietary mineral ligands and element bioavailability enhancement, particularly with respect to ligands from plant-derived food sources. This review highlights a novel perspective to consider various plant macro/micronutrients as prospective bioavailability enhancing ligands of three essential elements (Fe, Zn, and Ca). We also delineate the molecular mechanisms of the ligand-binding interactions underlying mineral bioaccessibility at the luminal level. We conclude that despite current understandings of some of the structure–activity relationships associated with strong mineral–ligand binding, the physiological links between ligands as element carriers and uptake at targeted sites throughout the gastrointestinal (GI) tract still require more research. The binding behavior of potential ligands in the human diet should be further elucidated and validated using pharmacokinetic approaches and GI models.     

Investigating the effect of production process of ball mill refiner on some physical quality parameters of compound chocolate: response surface methodology approach

Chocolate compound was produced using ball mill refiner, and the effect of agitator shaft speed and refining time on the physical quality parameters (particle size, colour and steady‐state rheology) of compound chocolate was determined using response surface methodology. The shaft speed and refining time range were selected between 40–60 r.p.m. and 10–30 min, respectively. Determination coefficient of the models established for particle size, Newtonian viscosity and colour parameters (brightness, chroma and hue angle) were found to be very close to unity. Increasing shaft speed and time induced a reduction in particle size and an increase in viscosity of the samples. Temperature sweep test was also performed, and the obtained data were successfully fitted to Arrhenius equation to calculate the corresponding parameters representing temperature dependency of the compounds. The results highlighted that the establishment of such models can provide essential information in terms of optimisation of production processes regarding usage purpose of the compound chocolate.