MicroRNA Expression in Clear Cell Renal Cell Carcinoma Cell Lines and Tumor Biopsies: Potential Therapeutic Targets

This study was carried out to quantitate the expression levels of microRNA-17, -19a, -34a, -155, and -210 (miRs) expressed in nine clear cell renal cell carcinoma (ccRCC) and one chromophobe renal cell carcinoma cell line with and without sarcomatoid differentiation, and in six primary kidney tumors with matching normal kidney tissues. The data in the five non-sarcomatoid ccRCC cell lines—RC2, CAKI-1, 786-0, RCC4, and RCC4/VHL—and in the four ccRCC with sarcomatoid differentiation—RCJ41T1, RCJ41T2, RCJ41M, and UOK-127—indicated that miR-17 and -19a were expressed at lower levels relative to miR-34a, -155, and -210. Compared with RPTEC normal epithelial cells, miR-34a, miR-155, and miR-210 were expressed at higher levels, independent of the sarcomatoid differentiation status and hypoxia-inducible factors 1α and 2α (HIFs) isoform expression. In the one chromophobe renal cell carcinoma cell line, namely, UOK-276 with sarcomatoid differentiation, and expressing tumor suppressor gene TP53, miR-34a, which is a tumor suppressor gene, was expressed at higher levels than miR-210, -155, -17, and -19a. The pilot results generated in six tumor biopsies with matching normal kidney tissues indicated that while the expression of miR-17 and -19a were similar to the normal tissue expression profile, miR-210, -155, -and 34a were expressed at a higher level. To confirm that differences in the expression levels of the five miRs in the six tumor biopsies were statistically significant, the acquisition of a larger sample size is required. Data previously generated in ccRCC cell lines demonstrating that miR-210, miR-155, and HIFs are druggable targets using a defined dose and schedule of selenium-containing molecules support the concept that simultaneous and concurrent downregulation of miR-210, miR-155, and HIFs, which regulate target genes associated with increased tumor angiogenesis and drug resistance, may offer the potential for the development of a novel mechanism-based strategy for the treatment of patients with advanced ccRCC.     

Renewable and Storage Energy Integration for Smart Grid Housing

This paper presents the design,implementation and testing of an embedded system that integrates solar and storage energy resources to smart homes within the smart microgrid.The proposed system provides the required home energy by installing renewable energy and storage devices.It also manages and schedules the power flow during peak and off-peak periods.In addition,a two-way communication protocol is developed to enable the home owners and the utility service provider to improve the energy flow and the consumption efficiency.The system can be an integral part for homes in a smart grid or smart microgrid power networks.A prototype for the proposed system was designed,implemented and tested by using a controlled load bank to simulate a scaled random real house consumption behavior.Three different scenarios were tested and the results and findings are reported.Moreover,data flow security among the home,home owners and utility server is developed to minimize cyber-attacks.     

Neurotoxicity of Chronic Co-Exposure of Lead and Ionic Liquid in Common Carp: Synergistic or Antagonistic?

Previous studies have indicated that the harmful heavy metal lead (Pb) contamination in aquatic systems has caused intelligence development disorders and nervous system function abnormalities in juveniles due to the increased permeability of the blood–brain barrier. Ionic liquids (ILs) are considered “green” organic solvents that can replace traditional organic solvents. Studies have found the presence of ILs in soil and water due to chemical applications or unintentional leakage. Therefore, what would happen if Pb interacted with ILs in a body of water? Could ILs enable Pb to more easily cross the blood–brain barrier? Therefore, we examined the combined exposure of Pb and ILs in common carp at low concentration (18.3 mg L⁻¹ of Pb(CH₃COO)₂•3 H₂O and 11 mg L⁻¹ of the IL 1-methyl-3-octylimidazolium chloride, 5% of their LC₅₀) for 28 days in the present study. The result of a neurobehavioral assay showed that chronic exposure of lead at lower concentrations significantly altered fish movement and neurobehaviors, indicating that lead exposure caused neurotoxicity in the carp. Increases in the neurotransmitter dopamine levels and injuries in the fish brain accounted for neurobehavioral abnormalities induced by lead exposure. Moreover, we also found that lead could easily cross the blood–brain barrier and caused significant bioaccumulation in the brain. Particularly, our study indicated that the ionic liquid could not synergistically promote blood–brain barrier permeability and hence failed to increase the absorption of lead in the fish brain, suggesting that the combined exposure of lead and ILs was not a synergistic effect but antagonism to the neurotoxicity. The results of this study suggested that ILs could recede the Pb induced neurotoxicity in fish.     

Preparation, characterization and application of advanced isoconversional kinetics to epoxy/1,4-Bis(3-aminopropoxy) butane/MWCNT nanocomposite

A novel nanocomposite consisting bisphenol A diglycidyl ether/1,4-Bis(3-aminopropoxy) butane (1,4-APB)/multiwall carbon nanotube (MWCNT) was synthesized and characterized. Kinetics of the reaction was described by applying differential scanning calorimetry (DSC) data to isoconversional methods of Flynn-Wall-Ozawa (FWO), advanced isoconversional method of Vyazovkin, and non-linear integral isoconversional algorithm (NLN). It was found that at the presence of MWCNT the thermal decomposition temperature increased by rising the curing temperature and time. Data from dynamic mechanical thermal analysis (DMTA) showed that the glass transition temperature of the cured nanocomposite is 7 °C higher than that value found for the system without carbon nanotube. Scanning electron microscopy (SEM) was used to observe the fracture surface morphology and results indicated evidence of the interfacial interaction improvement and adhesion strength due to good dispersion of MWCNT.     

Resistance of exterior three-dimensional walls to high velocity projectiles

There have been a number of recent civilian deaths due to ammunition from commonly used firearms penetrating exterior walls of residential, public, and commercial buildings. Experimental research was conducted to study the behavior of three-dimensional (3D) exterior walls when subjected to high velocity projectiles or bullets fired from a variety of firearms of different calibers. Four types of firearms were used, namely, a 9 mm gun, a 0.357 Magnum gun, an M16 machine gun, and a 7.62 Clashin Koff machine gun. The size of chips and the depth of penetrations produced by the fired bullets are documented and discussed. It was found that none of the fired bullets penetrated the two reinforced concrete layers of the exterior wall. In conclusion, 3D exterior walls provide an acceptable level of protection from ammunition fired from commonly used firearms.     

Cascaded waste‐heat recovery as a green technology for energy sustainability in power generation

In this work, the Cascaded waste‐heat recovery (WHR) is analyzed from the thermodynamic point of view. Typically, WHR is most effective with small gas turbines and old machines which have a relatively higher design mass flow per kW and higher exhaust temperatures than new designs. The working fluid used in the WHR technology is propane, which vaporizes and condenses at low temperatures. The temperature of the heat source, the outlet pressure of the two expanders, and the mass flow rate of the working fluid are assumed as working variables of the technology. The effect of these variables on the thermal efficiency and power output is evaluated. The obtained results are analyzed and discussed. The results of the calculation are also compared with similar published studies. The overall efficiency considering the gas turbine upstream ranges from about 35% up to 39%. The highest efficiency and power output of the WHR alone at 900 K heat source temperature, 800 kPa condenser pressure, and 100 kg/s mass‐flow rate are 30% and 18 MW, respectively, for two‐expander WHR, and 18% and 9 MW, respectively, for single expander WHR. Copyright © 2014 John Wiley & Sons, Ltd.     

Experimental characterization of the small strain shear modulus of soft clay stabilized with cement and nano-SiO2 using bender element tests

Bulletin of Engineering Geology and the Environment - The accurate and efficient modification of the static and dynamic properties of problematic soils is a primary concern in geotechnical...