Fungi and their secondary metabolites in water-damaged indoors after a major flood event in eastern Croatia

In winter and summer of 2016 and 2017, airborne fungi and house dust were collected in indoors of the village Gunja, which had been flooded, and the control village Gornji Stupnik (Croatia) in order to explore variations of fungal indoor levels, particularly Aspergilli section Nidulantes series Versicolores, as well as fungal metabolites in dust. Levels of airborne Aspergilli (Versicolores) were three times as high in winter and summer in Gunja than in the control village, while dustborne isolates were equally present in both locations. Sequencing of the calmodulin gene region revealed that among Aspergilli (Versicolores), A. jensenii and A. creber were dominant and together with A. puulaauensis, A. tennesseensis and A. venenatus produced sterigmatocystin and 5-methoxysterigmatocystin (HPLC coupled with mass spectrometry); A. amoenus, A. fructus, A. griseoaurantiacus, A. pepii, and A. protuberus produced sterigmatocystin but not 5-methoxysterigmatocystin; A. sydowii did not produce any of these toxins. A total of 75 metabolites related to Penicillium (29), Aspergillus (22), Fusarium (10), Alternaria (5), Stachybotrys (2), and other fungi (7) were detected in dust by liquid chromatography-tandem mass spectrometry. The majority of metabolites including sterigmatocystin and 5-methoxysterigmatocystin exhibited a higher prevalence in winter in Gunja.     

Ultrahigh Acceleration of Plasma Blocks by Nonlinear Forces for Side-On Laser Ignition of Solid Density Fusion Fuel

A fundamental difference of very high intensity laser interaction with plasmas from solid targets appears with lasing at picosecond (ps) pulse durations in contrast to pulses of nanosec- onds (ns). This can be seen from the more than 10,000 times higher acceleration with ps pulse du- rations than with thermal pressure determined interaction. A ps pulse duration produces instantly acting high-efficiency nonlinear (ponderomotive) electrodynamic force dominated acceleration in contrast to heating with longer pulses. The ps pulses accelerate high-density plasma blocks. This can be used by a new scheme of side-on driven laser fusion with generating a flame ignition in uncompressed fusion fuel of solid density resulting in a reaction velocity of more than 2000 km/s for DT.     

Vitamin D status in growing dairy goats and sheep: Influence of ultraviolet B radiation on bone metabolism and calcium homeostasis

The aim of this study was to investigate how controlled UVB irradiation in combination with reduced nutritional vitamin D (vitD) supply affects vitD status and Ca metabolism of growing goats and sheep. The hypothesis was that, like dairy cows, goats and sheep are able to compensate for the missing nutritional supply of vitD through endogenous production in the skin, with the consequence of a high vitD status and a balanced Ca homeostasis. Sixteen lambs and 14 goat kids aged 3 and a half months were housed in an UVB free environment and fed hay and a vitD-free concentrate over a period of 13 wk. One group of each species was exposed to UVB lamps daily during individual feeding; the other groups served as controls. Serum, urine, and feces samples were taken at the start and at a monthly interval. Serum was analyzed for vitD metabolites, bone markers, growth hormone, insulin-like growth factor I, Ca, and P. Apparent digestibility and urinary excretion of Ca and P were determined. The left metatarsus was analyzed by peripheral quantitative computer tomography for bone mineral density before starting and at the end of the trial. In wk 13, all animals were slaughtered and samples of skin, rumen, duodenum, kidney, and bone (metatarsus) were collected. Content of sterols of vitD synthesis in the skin, Ca flux rates in rumen and duodenum, expression of vitD receptor in duodenum and kidney, renal and intestinal gene expression of Ca transport proteins, and renal enzymes related to vitD metabolism were determined. The UVB exposure led to lower 7-dehydrocholesterol content in the skin and a better vitD status (higher serum 25-hydroxyvitamin D), but no signs of vitD deficiency were seen in the control groups and no effect of irradiation was detected in the analyzed parameters of Ca homeostasis. Differences between the 2 species were detected: lambs had a higher increase of bone mineral density, lower values of bone markers, growth hormone, and insulin-like growth factor I in serum and higher tachysterol and lower lumisterol content in skin compared with goat kids. The results indicated that growing lambs and goat kids are able to compensate for a vitD-reduced diet by cutaneous vitD synthesis when exposed to UVB irradiation and therefore to keep a high vitD status. In contrast, when a reduced vitD diet is combined with missing UVB exposure, the vitD status drops, but the experimental time was probably too short to induce a vitD deficiency or an effect on Ca homeostasis.     

Octree data structures and perspective imagery

The fundamental elements of perspective image generation from octree encoded graphics databases are examined. Procedures are presented for the encoding of spatially enumerated objects into an octree which is stored as a doubly-linked list. A perspective image rendering procedure is developed, based on raycasting, and involves a bidirectional traversal of the octree together with an adaptive level-of-detail control mechanism. The concepts and algorithms are verified by the synthesis of perspective terrain imagery.     

Complications of "slow-K" therapy

(1) The failure of ;Slow-K' tablets to disintegrate prevents rapid release but allows them to be trapped by their bulk in the intestine.(2) Two cases are reported. In the first the tablet was trapped in a caecal diverticulum and the patient developed an abcess. In the second, abdominal pain developed which subsided when ;Slow-K' was stopped. Later ;Slow-K' was again started and the patient developed dysphagia.(3) The possibility of abdominal complications with this treatment should be remembered.(4) Effervescent KC1 preparations may replace ;Slow-K' but KC1 supplementation may be necessary only in cardiac disease.     

Micropolar beam models for lattice grids with rigid joints

A simple, rational approach is presented for developing micropolar beam models for large repetitive beam-like planar lattices with rigid joints. The micropolar beam models have independent microrotation, and displacement fields and are characterized by their strain and kinetic energies, from which the equations of motion and boundary conditions can be derived. The procedure for developing the expression for the strain energy of the micropolar beam involves introducing basic assumptions regarding the variation of the displacement and microrotation components in the plane of the cross-section and obtaining effective elastic coefficients of the continuum in terms of the material properties and geometry of the original lattice structure. The high accuracy of the solutions obtained by the micropolar beam models is demonstrated by means of numerical examples.     

Spontaneous perseverative turning in rats with radiation-induced hippocampal damage.

This study found a new behavioral correlate of lesions specific to the dentate granule cell layer of the hippocampus: spontaneous perseverative turning. Irradiation of a portion of the neonatal rat cerebral hemispheres produced hypoplasia of the granule cell layer of the hippocampal dentate gyrus while sparing the rest of the brain. Radiation-induced damage to the hippocampal formation caused rats placed in bowls to spontaneously turn in long, slow bouts without reversals. Irradiated subjects also exhibited other behaviors characteristic of hippocampal damage (e.g., perseveration in spontaneous exploration of the arms of a T-maze, retarded acquisition of a passive avoidance task, and increased horizontal locomotion). These data extend previously reported behavioral correlates of fascia dentata lesions and suggest the usefulness of a bout analysis of spontaneous bowl turning as a measure of nondiscrete-trial spontaneous alternation and a sensitive additional indicator of radiation-induced hippocampal damage. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Large fluctuations in the disassembly rate of microtubules revealed by atomic force microscopy

Atomic force microscopy (AFM) in situ has been used to observe the cold disassembly dynamics of microtubules at a previously unrealised spatial resolution. Microtubules either electrostatically or covalently bound to aminosilane surfaces disassembled at room temperature under buffer solutions with no free tubulin present. This process was followed by taking sequential tapping-mode AFM images and measuring the change in the microtubule end position as a function of time, with an spatial accuracy down to +/-20nm and a temporal accuracy of +/-1s. As well as giving average disassembly rates on the order of 1-10 tubulin monomers per second, large fluctuations in the disassembly rate were revealed, indicating that the process is far from smooth and linear under these experimental conditions. The surface bound rates measured here are comparable to the rates for GMPCPP-tubulin microtubules free in solution, suggesting that inhibition of tubulin curvature through steric hindrance controls the average, relatively low disassembly rate. The large fluctuations in this rate are thought to be due to multiple pathways in the kinetics of disassembly with differing rate constants and/or stalling due to defects in the microtubule lattice. Microtubules that were covalently bound to the surface left behind the protofilaments covalently cross-linked to the aminosilane via glutaraldehyde during the disassembly process. Further work is needed to quantitatively assess the effects of surface binding on protofibril disassembly rates, reveal any differences in disassembly rates between the plus and minus ends and to enable assembly as well as disassembly to be imaged in the microscope fluid cell in real-time.     

Bounds on Flexural Properties and Buckling Response for Symmetrically Laminated Composite Plates

Nondimensional parameters and equations governing the buckling behavior of rectangular symmetrically laminated plates are presented that can be used to represent the buckling resistance, for plates made of all known structural materials, in a very general, insightful, and encompassing manner. In addition, these parameters can be used to assess the degree of plate orthotropy, to assess the importance of anisotropy that couples bending and twisting deformations, and to characterize quasi-isotropic laminates quantitatively. Bounds for these nondimensional parameters are also presented that are based on thermodynamics and practical laminate construction considerations. These bounds provide insight into potential gains in buckling resistance through laminate tailoring and composite-material development. As an illustration of this point, upper bounds on the buckling resistance of long rectangular orthotropic plates with simply supported or clamped edges and subjected to uniform axial compression, uniform shear, or pure in-plane bending loads are presented. The results indicate that the maximum gain in buckling resistance for tailored orthotropic laminates, with respect to the corresponding isotropic plate, is in the range of 26–36% for plates with simply supported edges, irrespective of the loading conditions. For the plates with clamped edges, the corresponding gains in buckling resistance are in the range of 9–12% for plates subjected to compression or pure in-plane bending loads and potentially up to 30% for plates subjected to shear loads.     

Role of cardiomyopathy in Noonan''s syndrome. Apropos of a case]

Dehydrocorydaline, an active principle of Corydalis bulbosa alkaloids, in concentrations of 10(-5) M to 5 x 10(-5)M inhibited relaxation and the concomitant release of (3H)-noradrenaline caused by 10(-4)M nicotine and electrical perivascular nerve stimulation in the taenia caecum of guinea pig. The same inhibitory effects were observed on contraction and release of (3H) noradrenaline in the sympathetic nerve-pulmonary artery preparation of rabbit. On the other hand, neither relaxation nor contraction caused by exogenously applied noradrenaline was affected. These results suggest that the inhibitory action of dehydrocorydaline on the relaxation or contraction, produced by nicotine and electrical nerve stimulation, is due to blockade of noradrenaline release from the adrenergic nerve terminals in both the taenia caecum and pulmonary artery. Participation of the adrenergic neuron blocking action of dehydrocorydaline in preventing experimental ulceration is discussed.