Alliance development in therapy for resolving child abuse issues.

Investigated client factors related to alliance development in an empirically supported, emotionally focused therapy for adult survivors of child abuse (EFT-AS). Despite severe abuse histories (emotional, physical, and/or sexual) and impairments in current interpersonal functioning, 33 clients (aged 19–72 yrs) reported strong early alliances, measured on the Working Alliance Inventory, comparable to nonabuse clients in a similar type of experiential therapy. Furthermore, alliance quality significantly improved over the course of EFT-AS, and early and late alliances each were associated with particular dimensions of posttreatment change. Severity of childhood physical and emotional abuse and neglect, measured on the Childhood Trauma Questionnaire, and the presence of Diagnostic and Statistical Manual of Mental Disorders-IV (DSM-IV) Axis II psychopathology were associated with early alliance difficulties. Axis II disturbance was the only client factor linked to alliance difficulties late in therapy, however, and none of the client prognostic variables were negatively associated with treatment outcome. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Unilateral Intrastriatal 6-Hydroxydopamine Lesion in Mice: A Closer Look into Non-Motor Phenotype and Glial Response

Parkinson’s disease (PD) is a prevalent movement disorder characterized by the progressive loss of dopaminergic neurons in substantia nigra pars compacta (SNpc). The 6-hydroxydopamine (6-OHDA) lesion is still one of the most widely used techniques for modeling Parkinson’s disease (PD) in rodents. Despite commonly used in rats, it can be challenging to reproduce a similar lesion in mice. Moreover, there is a lack of characterization of the extent of behavioral deficits and of the neuronal loss/neurotransmitter system in unilateral lesion mouse models. In this study, we present an extensive behavioral and histological characterization of a unilateral intrastriatal 6-OHDA mouse model. Our results indicate significant alterations in balance and fine motor coordination, voluntary locomotion, and in the asymmetry’s degree of forelimb use in 6-OHDA lesioned animals, accompanied by a decrease in self-care and motivational behavior, common features of depressive-like symptomatology. These results were accompanied by a decrease in tyrosine hydroxylase (TH)-labelling and dopamine levels within the nigrostriatal pathway. Additionally, we also identify a marked astrocytic reaction, as well as proliferative and reactive microglia in lesioned areas. These results confirm the use of unilateral intrastriatal 6-OHDA mice for the generation of a mild model of nigrostriatal degeneration and further evidences the recapitulation of key aspects of PD, thereby being suitable for future studies beholding new therapeutical interventions for this disease.     

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus

A pivotal role of type I interferons in systemic lupus erythematosus (SLE) is widely accepted. Type III interferons (IFN-λ) however, the most recently discovered cytokines grouped within the interferon family, have not been extensively studied in lupus disease models yet. Growing evidence suggests a role for IFN-λ in regulating both innate and adaptive immune responses, and increased serum concentrations have been described in multiple autoimmune diseases including SLE. Using the pristane-induced lupus model, we found that mice with defective IFN-λ receptors (Ifnlr1^−/−) showed increased survival rates, decreased lipogranuloma formation and reduced anti-dsDNA autoantibody titers in the early phase of autoimmunity development compared to pristane-treated wild-type mice. Moreover, Ifnlr1^−/− mice treated with pristane had reduced numbers of inflammatory mononuclear phagocytes and cNK cells in their kidneys, resembling untreated control mice. Systemically, circulating B cells and monocytes (CD115⁺Ly6C⁺) were reduced in pristane-treated Ifnlr1^−/− mice. The present study supports a significant role for type III interferons in the pathogenesis of pristane-induced murine autoimmunity as well as in systemic and renal inflammation. Although the absence of type III interferon receptors does not completely prevent the development of autoantibodies, type III interferon signaling accelerates the development of autoimmunity and promotes a pro-inflammatory environment in autoimmune-prone hosts.     

Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂) is an essential plasma membrane component involved in several cellular functions, including membrane trafficking and cytoskeleton organization. This function multiplicity is partially achieved through a dynamic spatiotemporal organization of PI(4,5)P₂ within the membrane. Here, we use a Förster resonance energy transfer (FRET) approach to quantitatively assess the extent of PI(4,5)P₂ confinement within the plasma membrane. This methodology relies on the rigorous evaluation of the dependence of absolute FRET efficiencies between pleckstrin homology domains (PH_PLCδ) fused with fluorescent proteins and their average fluorescence intensity at the membrane. PI(4,5)P₂ is found to be significantly compartmentalized at the plasma membrane of HeLa cells, and these clusters are not cholesterol-dependent, suggesting that membrane rafts are not involved in the formation of these nanodomains. On the other hand, upon inhibition of actin polymerization, compartmentalization of PI(4,5)P₂ is almost entirely eliminated, showing that the cytoskeleton network is the critical component responsible for the formation of nanoscale PI(4,5)P₂ domains in HeLa cells.     

Elucidating the process of hydrogen generation from the reaction of sodium hydroxide solution and ferrosilicon

For the first time, the process of hydrogen evolution from ferrosilicon 75 using sodium hydroxide solution has been investigated as a function of temperature using a combination of X‐ray photoelectron spectroscopy, X‐ray diffraction and physical measurements. Ferrosilicon 75, a mixture of silicon (~50 wt.%) and iron disilicide (~50 wt.%), has been shown to produce hydrogen by the action of sodium hydroxide solution on the silicon only, with the iron disilicide acting in the role of spectator/protector species for the silicon. Neither iron disilicide alone nor ferrosilicon 45, which does not contain a pure metallic silicon phase, was found to generate hydrogen under similar reaction conditions, further indicating that the presence of a pure metallic silicon phase is essential for hydrogen generation. As the iron disilicide acts as a diluent for the active silicon, it is hypothesized that this would result in a slower release of hydrogen than that which would be obtained from the reaction of silicon alone, which may be useful for applications which require a long‐term, sustained release of hydrogen. A hydrogen yield of 462.5 mL/g and a maximum hydrogen generation rate of 83 mL/min g were obtained within 10 min of reaction with 40 wt.% NaOH at 348 K. © 2017 The Authors. International Journal of Energy Research Published by John Wiley & Sons Ltd.     

Risk in infancy: Origins and implications.

This article focuses on biological risks that can adversely influence development during infancy and later. In the first part of the article, the origins of risks and their potential consequences are discussed relative to prepregnancy, prenatal, perinatal, and postnatal periods. Epidemiological data are presented. The second part of the article addresses issues pertaining to assessment of infant development and interventions provided for infants whose development may be in jeopardy. Finally, goals for prevention are highlighted and the need for a nationally derived data base on risk and outcomes is emphasized. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Eye movement desensitization and reprocessing: Four case studies of a new tool for executive coaching and restoring employee performance after setbacks.

The effects of eye movement desensitization and reprocessing (EMDR) integrated into executive coaching are reported in 4 case studies illustrating varied job titles and industries. Participants received 1–10 hrs of coaching in which EMDR was used to desensitize an upsetting event that had impaired their performance at work. Outcomes indicated that EMDR desensitized the disturbing incident and that participants shifted their negative view to a more positive one. Work performance was restored or enhanced. In the 4th case EMDR appeared to decrease anxiety about job interviewing and the participant reported a satisfactory result. Findings suggest that EMDR is a promising adjunct to coaching for workplace performance enhancement. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The benefits of positive illusions: Idealization and the construction of satisfaction in close relationships.

It is proposed that satisfaction is associated with idealistic, rather than realistic, perceptions of one's partner. To provide baselines for assessing relationship illusions, both members of married and dating heterosexual couples were asked to rate themselves and their partners on a variety of interpersonal attributes. Participants also rated the typical and ideal partner on these attributes. Path analyses revealed that individuals' impressions of their partners were more a mirror of their self-images and ideals than a reflection of their partners' self-reported attributes. Overall, intimates saw their partners in a more positive light than their partners saw themselves. Furthermore, these idealized constructions predicted greater satisfaction. Individuals were happier in their relationships when they idealized their partners and their partners idealized them. Taken together, these results suggest that a certain degree of idealization or illusion may be a critical feature of satisfying dating and even marital relationships. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Wireless Internet and student-centered learning: A Partial Least-Squares model

Wireless Internet technology is gaining a foothold on more and more campuses, yet few studies have investigated how wireless Internet supports and enhances a student-centered learning environment. This study seeks to fill the gap by developing an instrument to measure how wireless Internet supports student-centered learning. A web survey was designed and administered at a public university in the USA where participants were student wireless Internet users. Structural equation modeling employing Partial Least-Squares approach was used to test the research model. The model explains 77% of the variance (R²) in student-centered learning environment. The survey results suggest that wireless Internet has a positive and significant influence on student-centered learning in three dimensions: pedagogical, technological, and cultural learning.