全文获取类型
收费全文 | 2819篇 |
免费 | 188篇 |
国内免费 | 5篇 |
专业分类
电工技术 | 13篇 |
综合类 | 2篇 |
化学工业 | 906篇 |
金属工艺 | 18篇 |
机械仪表 | 56篇 |
建筑科学 | 129篇 |
能源动力 | 56篇 |
轻工业 | 580篇 |
水利工程 | 36篇 |
石油天然气 | 9篇 |
无线电 | 135篇 |
一般工业技术 | 433篇 |
冶金工业 | 298篇 |
原子能技术 | 8篇 |
自动化技术 | 333篇 |
出版年
2024年 | 11篇 |
2023年 | 69篇 |
2022年 | 211篇 |
2021年 | 255篇 |
2020年 | 106篇 |
2019年 | 117篇 |
2018年 | 138篇 |
2017年 | 111篇 |
2016年 | 107篇 |
2015年 | 116篇 |
2014年 | 111篇 |
2013年 | 195篇 |
2012年 | 172篇 |
2011年 | 217篇 |
2010年 | 124篇 |
2009年 | 143篇 |
2008年 | 121篇 |
2007年 | 123篇 |
2006年 | 87篇 |
2005年 | 72篇 |
2004年 | 55篇 |
2003年 | 51篇 |
2002年 | 38篇 |
2001年 | 12篇 |
2000年 | 18篇 |
1999年 | 23篇 |
1998年 | 17篇 |
1997年 | 22篇 |
1996年 | 14篇 |
1995年 | 11篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 11篇 |
1990年 | 8篇 |
1989年 | 8篇 |
1988年 | 10篇 |
1987年 | 6篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 5篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1964年 | 3篇 |
1958年 | 2篇 |
排序方式: 共有3012条查询结果,搜索用时 15 毫秒
61.
Gaia Codolo Nicola Facchinello Nicole Papa Ambra Bertocco Sara Coletta Clara Benna Luigi DallOlmo Simone Mocellin Natascia Tiso Marina de Bernard 《International journal of molecular sciences》2022,23(3)
The Helicobacter pylori Neutrophil Activating Protein (HP-NAP) is endowed with immunomodulatory properties that make it a potential candidate for anticancer therapeutic applications. By activating cytotoxic Th1 responses, HP-NAP inhibits the growth of bladder cancer and enhances the anti-tumor activity of oncolytic viruses in the treatment of metastatic breast cancer and neuroendocrine tumors. The possibility that HP-NAP exerts its anti-tumor effect also by modulating the activity of innate immune cells has not yet been explored. Taking advantage of the zebrafish model, we examined the therapeutic efficacy of HP-NAP against metastatic human melanoma, limiting the observational window to 9 days post-fertilization, well before the maturation of the adaptive immunity. Human melanoma cells were xenotransplanted into zebrafish embryos and tracked in the presence or absence of HP-NAP. The behavior and phenotype of macrophages and the impact of their drug-induced depletion were analyzed exploiting macrophage-expressed transgenes. HP-NAP administration efficiently inhibited tumor growth and metastasis and this was accompanied by strong recruitment of macrophages with a pro-inflammatory profile at the tumor site. The depletion of macrophages almost completely abrogated the ability of HP-NAP to counteract tumor growth. Our findings highlight the pivotal role of activated macrophages in counteracting melanoma growth and support the notion that HP-NAP might become a new biological therapeutic agent for the treatment of metastatic melanomas. 相似文献
62.
63.
Katarina A. Jones Allison J. Richard J. Michael Salbaum Susan Newman Richard Carmouche Sara Webb Annadora J. Bruce-Keller Jacqueline M. Stephens Shawn R. Campagna 《International journal of molecular sciences》2022,23(7)
Herbal remedies are increasing in popularity as treatments for metabolic conditions such as obesity and Type 2 Diabetes. One potential therapeutic option is fenugreek seeds (Trigonella foenum-graecum), which have been used for treating high cholesterol and Type 2 diabetes. A proposed mechanism for these benefits is through alterations in the microbiome, which impact mammalian host metabolic function. This study used untargeted metabolomics to investigate the fenugreek-induced alterations in the intestinal, liver, and serum profiles of mice fed either a 60% high-fat or low-fat control diet each with or without fenugreek supplementation (2% w/w) for 14 weeks. Metagenomic analyses of intestinal contents found significant alterations in the relative composition of the gut microbiome resulting from fenugreek supplementation. Specifically, Verrucomicrobia, a phylum containing beneficial bacteria which are correlated with health benefits, increased in relative abundance with fenugreek. Metabolomics partial least squares discriminant analysis revealed substantial fenugreek-induced changes in the large intestines. However, it was observed that while the magnitude of changes was less, significant modifications were present in the liver tissues resulting from fenugreek supplementation. Further analyses revealed metabolic processes affected by fenugreek and showed broad ranging impacts in multiple pathways, including carnitine biosynthesis, cholesterol and bile acid metabolism, and arginine biosynthesis. These pathways may play important roles in the beneficial effects of fenugreek. 相似文献
64.
Sara Lombardo Gaetano Pandino Giovanni Mauromicale Matthias Knödler Reinhold Carle Andreas Schieber 《Food chemistry》2010
Globe artichoke is an ancient herbaceous plant native to the Mediterranean Basin. The edible part of the plant (head) is particularly rich in polyphenols, whose therapeutic properties are well documented. A field experiment was conducted in Sicily (south Italy) to examine the influence of genotype and harvest time on the polyphenol content and profile of different head parts. The concentrations of 19 phenolic compounds were determined by HPLC-DAD-ESI/MSn analysis. It was observed that individual phenolic substances were preferentially accumulated in specific head parts and genotypes. Apigenin 7-O-glucuronide was found to be the major flavonoid, with 6298 mg kg−1 DM in ‘Romanesco clone C3’ receptacle, whereas chlorogenic acid represented the main caffeoylquinic acid, reaching 14841 mg kg−1 DM in the inner bracts of ‘Violetto di Sicilia’. Our findings prove also the influence of climatic conditions on the phenolic profile and thus suggest giving specific consideration to harvest time. 相似文献
65.
Sara Gonzlez-García Carles M. Gasol Xavier Gabarrell Joan Rieradevall Mª Teresa Moreira Gumersindo Feijoo 《Renewable & Sustainable Energy Reviews》2009,13(9):2613-2620
One of the main challenges faced by mankind in the 21st century is to meet the increasing demand for energy requirements by means of a more sustainable energy supply. In countries that are net fossil fuel importers, expectation about the benefit of using alternative fuels on reducing oil imports is the primary driving force behind efforts to promote its production and use. Spain is scarce in domestic energy sources and more than 50% of the energy used is fossil fuel based. The promotion of renewable energies use is one of the principal vectors in the Spanish energy policy. Selected herbaceous crops such as Brassica carinata are currently under study as potential energy sources. Its biomass can be considered as potential feedstock to ethanol conversion by an enzymatic process due to the characteristics of its composition, rich in cellulose and hemicellulose. This paper aims to analyse the environmental performance of two ethanol-based fuel applications (E10 and E85) in a passenger car (E10 fuel: a mixture of 10% ethanol and 90% gasoline by volume; E85 fuel: a mixture of 85% ethanol and 15% gasoline by volume) as well as their comparison with conventional gasoline as transport fuel. Two types of functional units are applied in this study: ethanol production oriented and travelling distance oriented functional units in order to reflect the availability or not of ethanol supply. E85 seems to be the best alternative when ethanol production based functional unit is considered in terms of greenhouse gas (GHG) emissions and E10 in terms of non-renewable energy resources use. Nevertheless, E85 offers the best environmental performance when travelling distance oriented functional unit is assumed in both impacts. In both functional unit perspectives, the use of ethanol-based fuels reduces the global warming and fossil fuels consumption. However, the contributions to other impact indicators (e.g. acidification, eutrophication and photochemical oxidants formation) were lower for conventional gasoline.Life Cycle Assessment (LCA) procedure helps to identify the key areas in the B. carinata ethanol production life cycle where the researchers and technicians need to work to improve the environmental performance. Technological development could help in lowering both the environmental impact and the prices of the ethanol fuels. 相似文献
66.
Phenol‐enriched olive oils modify paraoxonase‐related variables: A randomized,crossover, controlled trial 下载免费PDF全文
67.
In recent years, droughts with increasing severity and frequency have been experienced around the world due to climate change
effects. Water planning and management during droughts needs to deal with water demand variability, uncertainties in streamflow
prediction, conflicts over water resources allocation, and the absence of necessary emergency schemes in drought situations.
Reservoirs could play an important role in drought mitigation; therefore, development of an algorithm for operation of reservoirs
in drought periods could help to mitigate the drought impacts by reducing the expected water shortages. For this purpose,
the probable drought’s characteristics and their variations in response to factors such as climate change should be incorporated.
This study aims at developing a contingency planning scheme for operation of reservoirs in drought periods using hedging rules
with the objective of decreasing the maximum water deficit. The case study for evaluation of the performance of the proposed
algorithm is the Sattarkhan reservoir in the Aharchay watershed, located in the northwestern part of Iran. The trend evaluations
of the hydro-climatic variables show that the climate change has already affected streamflow in the region and has increased
water scarcity and drought severity. To incorporate the climate change study in reservoir planning; streamflow should be simulated
under climate change impacts. For this purpose, the climatic variables including temperature and precipitation in the future
under climate change impacts are simulated using downscaled GCM (General Circulation Model) outputs to derive scenarios for
possible future drought events. Then a hydrological model is developed to simulate the river streamflow, based on the downscaled
data. The results show that the proposed methodology leads to less water deficit and decreases the drought damages in the
study area. 相似文献
68.
Sobia Noreen Sara Hasan Shazia Akram Ghumman Syed Nasir Abbas Bukhari Bushra Ijaz Huma Hameed Huma Iqbal Afeefa Aslam Mervat Abdelaziz Mohamed Elsherif Shazia Noureen Hasan Ejaz 《International journal of molecular sciences》2022,23(5)
The rapid progression in biomaterial nanotechnology apprehends the potential of non-toxic and potent polysaccharide delivery modules to overcome oral chemotherapeutic challenges. The present study is aimed to design, fabricate and characterize polysaccharide nanoparticles for methotrexate (MTX) delivery. The nanoparticles (NPs) were prepared by Abelmoschus esculentus mucilage (AEM) and chitosan (CS) by the modified coacervation method, followed by ultra-sonification. The NPs showed much better pharmaceutical properties with a spherical shape and smooth surface of 213.4–254.2 nm with PDI ranging between 0.279–0.485 size with entrapment efficiency varying from 42.08 ± 1.2 to 72.23 ± 2.0. The results revealed NPs to possess positive zeta potential and a low polydispersity index (PDI). The in-vitro drug release showed a sustained release of the drug up to 32 h with pH-dependence. Blank AEM -CS NPs showed no in-vivo toxicity for a time duration of 14 days, accompanied by high cytotoxic effects of optimized MTX loaded NPs against MCF-7 and MD-MBA231 cells by MTT assay. In conclusion, the findings advocated the therapeutic potential of AEM/CS NPs as an efficacious tool, offering a new perspective for pH-responsive routing of anticancer drugs with tumor cells as a target. 相似文献
69.
Arianna Piersanti Tamara Tavoloni Eleonora Bastari Carmela Lestingi Sara Romanelli Rosanna Rossi Giorgio Saluti Simone Moretti Roberta Galarini 《Food Analytical Methods》2018,11(2):355-366
This paper describes the development and validation of an analytical method to determine 15 polybrominated diphenyl ethers (PBDEs) in fish and shellfish tissues with gas chromatography coupled to triple quadrupole mass spectrometry using electron impact source (GC-EI-MS/MS). A QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe)-like procedure was applied; the extract was cleaned up on an acidic Extrelut-NT3 column connected on top of a silica cartridge and further purified with gel permeation chromatography (GPC). The SIM acquisition proved to yield better results than traditional MRM approach for octa-, nona-, and deca-brominated congeners. The validation study was carried out in inter-laboratory conditions. The average recoveries were in the range 66–118%, with relative standard deviations generally lower than or equal to 22%. Quality assurance and quality control practices were thoroughly described and highlighted. These long and tedious practices are fundamental to obtain reliable quantitative data during food monitoring programs and, therefore, to support daily intake assessments. Finally, the procedure was applied to the analysis of marine fish samples collected in local markets. Mackerel and mullet were the most contaminated species. 相似文献
70.
The objective of any night vision system is to enable a person to see in the dark. A low-contrast image puts a contrast constraint on the human observer visibility at night. This is the basic reason for the large number of accidents at night. This research presents two proposed approaches to enhance the visibility of the infrared (IR) night vision images through an efficient histogram processing. The first approach is based on contrast limited adaptive histogram equalization. The second proposed approach depends on histogram matching. The histogram matching uses a reference visual image for converting night vision images into good quality images. The obtained results are evaluated with quality metrics such as entropy, average gradient, contrast improvement factor and sobel edge magnitude. 相似文献