A novel consolidation method to measure powder flow properties using a small amount of material

Bulk flow property characterization often requires large powder samples (tens to hundreds of grams). However, many applications have limited sample availability, due to cost, material availability, safety concerns, etc. Therefore, reducing the amount of required material is of interest. A novel compressibility method is introduced using less than 50 mg, for the materials studied here. The effect of particle size and cohesion due to capillary forces are determined using a small‐scale compressibility cell mounted on a texture analyzer. It is found that the powder bed consolidation occurred in two regimes, described using the Walker and Heckel equations. The small‐scale compressibility method was compared to known behavior at larger scales and validated against the FT4 compressibility test. It was found that bulk behavior could be observed using the small‐scale compressibility method. Additional behavior caused by small‐scale events, which are averaged out in large‐scale measurements, are revealed in the small‐scale device introduced here. © 2016 American Institute of Chemical Engineers AIChE J, 62: 4193–4200, 2016     

Runoff of pharmaceuticals and personal care products following application of biosolids to an agricultural field

Municipal biosolids are a source of nutrients for crop production. Beneficial Management Practices (BMPs) can be used to minimize the risk of contamination of adjacent water resources with chemical or microbial agents that are of public or environmental health concern. In this field study, we applied biosolids slurry at a commercial rate using either subsurface injection or broadcast application followed by incorporation. Precipitation was simulated at 1, 3, 7, 22, 36 and 266 days post-application on 2 m² microplots to evaluate surface runoff of 9 model pharmaceuticals and personal care products (PPCPs), atenolol, carbamazepine, cotinine, gemfibrozil, naproxen, ibuprofen, acetaminophen, sulfamethoxazole and triclosan. In runoff from the injected plots, concentrations of the model PPCPs were generally below the limits of quantitation. In contrast, in the broadcast application treatment, the concentrations of atenolol, carbamazepine, cotinine, gemfibrozil, naproxen, sulfamethoxazole and triclosan on the day following application ranged from 70-1477 ng L^− 1 in runoff and generally declined thereafter with first order kinetics. The total mass of PPCPs mobilized in surface runoff per m² of the field ranged from 0.63 µg for atenolol to 21.1 µg for ibuprofen. For ibuprofen and acetaminophen, concentrations in runoff first decreased and then increased, suggesting that these drugs were initially chemically or physically sequestered in the biosolids slurry, and subsequently released in the soil. Carbamazepine and triclosan were detected at low concentrations in a runoff event 266 days after broadcast application. Overall, this study showed that injection of biosolids slurry below the soil surface could effectively eliminate surface runoff of PPCPs.     

Cold Spray Deposition of Freestanding Inconel Samples and Comparative Analysis with Selective Laser Melting

Cold spray offers the possibility of obtaining almost zero-porosity buildups with no theoretical limit to the thickness. Moreover, cold spray can eliminate particle melting, evaporation, crystallization, grain growth, unwanted oxidation, undesirable phases and thermally induced tensile residual stresses. Such characteristics can boost its potential to be used as an additive manufacturing technique. Indeed, deposition via cold spray is recently finding its path toward fabrication of freeform components since it can address the common challenges of powder-bed additive manufacturing techniques including major size constraints, deposition rate limitations and high process temperature. Herein, we prepared nickel-based superalloy Inconel 718 samples with cold spray technique and compared them with similar samples fabricated by selective laser melting method. The samples fabricated using both methods were characterized in terms of mechanical strength, microstructural and porosity characteristics, Vickers microhardness and residual stresses distribution. Different heat treatment cycles were applied to the cold-sprayed samples in order to enhance their mechanical characteristics. The obtained data confirm that cold spray technique can be used as a complementary additive manufacturing method for fabrication of high-quality freestanding components where higher deposition rate, larger final size and lower fabrication temperatures are desired.     

Development of a Demand Driven Hydro-climatic Model for Drought Planning

In recent years, droughts with increasing severity and frequency have been experienced around the world due to climate change effects. Water planning and management during droughts needs to deal with water demand variability, uncertainties in streamflow prediction, conflicts over water resources allocation, and the absence of necessary emergency schemes in drought situations. Reservoirs could play an important role in drought mitigation; therefore, development of an algorithm for operation of reservoirs in drought periods could help to mitigate the drought impacts by reducing the expected water shortages. For this purpose, the probable drought’s characteristics and their variations in response to factors such as climate change should be incorporated. This study aims at developing a contingency planning scheme for operation of reservoirs in drought periods using hedging rules with the objective of decreasing the maximum water deficit. The case study for evaluation of the performance of the proposed algorithm is the Sattarkhan reservoir in the Aharchay watershed, located in the northwestern part of Iran. The trend evaluations of the hydro-climatic variables show that the climate change has already affected streamflow in the region and has increased water scarcity and drought severity. To incorporate the climate change study in reservoir planning; streamflow should be simulated under climate change impacts. For this purpose, the climatic variables including temperature and precipitation in the future under climate change impacts are simulated using downscaled GCM (General Circulation Model) outputs to derive scenarios for possible future drought events. Then a hydrological model is developed to simulate the river streamflow, based on the downscaled data. The results show that the proposed methodology leads to less water deficit and decreases the drought damages in the study area.     

pH Responsive Abelmoschus esculentus Mucilage and Administration of Methotrexate: In-Vitro Antitumor and In-Vivo Toxicity Evaluation

The rapid progression in biomaterial nanotechnology apprehends the potential of non-toxic and potent polysaccharide delivery modules to overcome oral chemotherapeutic challenges. The present study is aimed to design, fabricate and characterize polysaccharide nanoparticles for methotrexate (MTX) delivery. The nanoparticles (NPs) were prepared by Abelmoschus esculentus mucilage (AEM) and chitosan (CS) by the modified coacervation method, followed by ultra-sonification. The NPs showed much better pharmaceutical properties with a spherical shape and smooth surface of 213.4–254.2 nm with PDI ranging between 0.279–0.485 size with entrapment efficiency varying from 42.08 ± 1.2 to 72.23 ± 2.0. The results revealed NPs to possess positive zeta potential and a low polydispersity index (PDI). The in-vitro drug release showed a sustained release of the drug up to 32 h with pH-dependence. Blank AEM -CS NPs showed no in-vivo toxicity for a time duration of 14 days, accompanied by high cytotoxic effects of optimized MTX loaded NPs against MCF-7 and MD-MBA231 cells by MTT assay. In conclusion, the findings advocated the therapeutic potential of AEM/CS NPs as an efficacious tool, offering a new perspective for pH-responsive routing of anticancer drugs with tumor cells as a target.     

Hedgehog Signalling Modulates Immune Response and Protects against Experimental Autoimmune Encephalomyelitis

The Hedgehog (Hh) pathway is essential for the embryonic development and homeostatic maintenance of many adult tissues and organs. It has also been associated with some functions of the innate and adaptive immune system. However, its involvement in the immune response has not been well determined. Here we study the role of Hh signalling in the modulation of the immune response by using the Ptch-1-LacZ^+/− mouse model (hereinafter referred to as ptch^+/−), in which the hemizygous inactivation of Patched-1, the Hh receptor gene, causes the constitutive activation of Hh response genes. The in vitro TCR stimulation of spleen and lymph node (LN) T cells showed increased levels of Th2 cytokines (IL-4 and IL-10) in ptch^+/−cells compared to control cells from wild-type (wt) littermates, suggesting that the Th2 phenotype is favoured by Hh pathway activation. In addition, CD4⁺ cells secreted less IL-17, and the establishment of the Th1 phenotype was impaired in ptch^+/− mice. Consistently, in response to an inflammatory challenge by the induction of experimental autoimmune encephalomyelitis (EAE), ptch^+/− mice showed milder clinical scores and more minor spinal cord damage than wt mice. These results demonstrate a role for the Hh/ptch pathway in immune response modulation and highlight the usefulness of the ptch^+/− mouse model for the study of T-cell-mediated diseases and for the search for new therapeutic strategies in inflammatory diseases.     

Dysfunctional cGMP Signaling Leads to Age-Related Retinal Vascular Alterations and Astrocyte Remodeling in Mice

The nitric oxide–guanylyl cyclase-1–cyclic guanylate monophosphate (NO–GC-1–cGMP) pathway is integral to the control of vascular tone and morphology. Mice lacking the alpha catalytic domain of guanylate cyclase (GC1^−/−) develop retinal ganglion cell (RGC) degeneration with age, with only modest fluctuations in intraocular pressure (IOP). Increasing the bioavailability of cGMP in GC1^−/− mice prevents neurodegeneration independently of IOP, suggesting alternative mechanisms of retinal neurodegeneration. In continuation to these studies, we explored the hypothesis that dysfunctional cGMP signaling leads to changes in the neurovascular unit that may contribute to RGC degeneration. We assessed retinal vasculature and astrocyte morphology in young and aged GC1^−/− and wild type mice. GC1^−/− mice exhibit increased peripheral retinal vessel dilation and shorter retinal vessel branching with increasing age compared to Wt mice. Astrocyte cell morphology is aberrant, and glial fibrillary acidic protein (GFAP) density is increased in young and aged GC1^−/− mice, with areas of dense astrocyte matting around blood vessels. Our results suggest that proper cGMP signaling is essential to retinal vessel morphology with increasing age. Vascular changed are preceded by alterations in astrocyte morphology which may together contribute to retinal neurodegeneration and loss of visual acuity observed in GC1^−/− mice.     

Understanding challenges in the front lines of home health care: A human-systems approach

A human-systems perspective is a fruitful approach to understanding home health care because it emphasizes major individual components of the system – persons, equipment/technology, tasks, and environments – as well as the interaction between these components. The goal of this research was to apply a human-system perspective to consider the capabilities and limitations of the persons, in relation to the demands of the tasks and equipment/technology in home health care. Identification of challenges and mismatches between the person(s) capabilities and the demands of providing care provide guidance for human factors interventions. A qualitative study was conducted with 8 home health Certified Nursing Assistants and 8 home health Registered Nurses interviewed about challenges they encounter in their jobs. A systematic categorization of the challenges the care providers reported was conducted and human factors recommendations were proposed in response, to improve home health. The challenges inform a human-systems model of home health care.     

A Review of Pathways for Building Fire Spread in the Wildland Urban Interface Part II: Response of Components and Systems and Mitigation Strategies in the United States

Structure loss in wildland fires has significantly increased over the past few decades, affected by increased development in rural areas, changing fuel management policies, and climate change, all of which are projected to increase in the future. This paper is Part II of a two-part review, which presents a summary of fundamental and applied research on pathways to fire spread in the wildland urban interface. Part I discussed the fundamentals of wildland fire spread via radiative heat transfer, direct flame contact, and firebrand exposure. Here in Part II, we cover the response of building components and systems, as well as mitigation strategies used to prevent fire spread into and within communities in the United States. Post-fire investigations, full-scale structural testing, individual component testing, and combined systems or assembly testing have been used to identify building component and system vulnerabilities such as roofs, vents, siding, decks, fences, and mulch. Using results from these tests and investigations at different scales, some knowledge has been gained on specific vulnerabilities and the effectiveness of mitigation strategies, but a quantitative framework has not yet been established. On a community level, the layout of structures and the space between them has been shown to be incredibly important in mitigating wildfire risk. More locally, defensible space around homes has been effective in mitigating exposure from both radiation and direct flame contact. Firebrands still remain a challenge; however, many design recommendations have been proposed to harden structures against firebrand exposures. Recommendations for future research and development are also presented.