Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli

Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L⁻¹ PSP in 48 h at 20 °C directly from carbon feedstock.     

Digitale Zwillinge in der Bioprozesstechnik – Chancen und Möglichkeiten

The digitalization of processes is one of the currently dominating topics and missions in both industrial production and scientific research. In biotechnology, these efforts have enormous potential to optimize existing bioprocesses or to develop new bioprocesses for upcoming challenges. In this review, the state-of-art of digitalization in bioprocess engineering is considered, the terms digital twin and digital shadow are characterized, and an outlook on future Lab 4.0 concepts is given.     

Fostering knowledge sharing: Design principles for persuasive digital technologies in open innovation projects

Integrating knowledge is crucial for open innovation, and digital technologies can play a central role because they support knowledge sharing. In open innovation projects, in particular, little is known about the role of technology. Here, the individual behaviour of users is taken into account concerning the extent to which knowledge is shared. Therefore, persuasive technologies offer the potential to foster sharing. In particular, to facilitate the construction of future digital technologies, this study applies a design science research approach to create and analyse artefacts as a research contribution and develop design principles as a step towards a nascent design theory. We present insights from the design and application of three artefacts in different stages of open innovation processes. Results show that digital technologies can be used for various purposes: to build a common understanding, support design phases, communicate ideas and simplify the application of the technology for the user. Our research provides insights into the role of digital technologies for knowledge sharing in open innovation projects, and four design principles are found to facilitate the construction of future persuasive digital technologies for open innovation projects.     

Van der Waals Heteroepitaxy of GaSe and InSe,Quantum Wells,and Superlattices

Bandgap engineering and quantum confinement in semiconductor heterostructures provide the means to fine-tune material response to electromagnetic fields and light in a wide range of the spectrum. Nonetheless, forming semiconductor heterostructures on lattice-mismatched substrates is a challenge for several decades, leading to restrictions for device integration and the lack of efficient devices in important wavelength bands. Here, it is shown that the van der Waals epitaxy of 2D GaSe and InSe heterostructures occur on substrates with substantially different lattice parameters, namely silicon and sapphire. The GaSe/InSe heteroepitaxy is applied in the growth of quantum wells and superlattices presenting photoluminescence and absorption related to interband transitions.