Kinetic analysis of cellular internalization and expulsion of unstructured D-chirality cell penetrating peptides

Most cell penetrating peptides (CPPs) are unstructured and susceptible to proteolytic degradation. One alternative is to incorporate D-chirality amino acids into unstructured CPPs to allow for enhanced uptake and intracellular stability. This work investigates CPP internalization using a series of time, concentration, temperature, and energy dependent studies, resulting in a three-fold increase in uptake and 50-fold increase in stability of D-chirality peptides over L-chirality counterparts. CPP internalization occurred via a combination of direct penetration and endocytosis, with a percentage of internalized CPP expelling from cells in a time-dependent manner. Mechanistic studies identified that cells exported the intact internalized D-chirality CPPs via an exocytosis independent pathway, analogous to a direct penetration method out of the cells. These findings highlight the potential of a D-chirality CPP as bio-vector in therapeutic and biosensing applications, but also identify a new expulsion method suggesting a relationship between uptake kinetics, intracellular stability, and export kinetics.     

Modeling drop breakage using the full energy spectrum and a specific realization of turbulence anisotropy

The assumption of homogeneous isotropic turbulence when modeling drop breakage in industrially relevant geometries is questionable. We describe the development of an anisotropic breakage model, where the anisotropy is introduced via the inclusion of a perturbed turbulence spectrum. The selection of the perturbed spectrum is itself motivated by our previous large-eddy simulations of high-pressure homogenizers. The model redistributes energy from small to large scales, and assumes that the anisotropic part of the Reynolds stresses is confined to the energy-containing range. The second-order structure function arising from the perturbed spectrum is used in the standard framework of Coulaloglou and Tavlarides to calculate breakage frequency. While the base model exhibits non-monotonic behavior (by predicting a maximum value for a certain drop size), the effect of anisotropy is shown to increase breakage frequency in length scales larger than this peak, thereby reducing non-monotonicity. This effect is more pronounced for small turbulence Reynolds numbers.     

Host Plant Constancy in Ovipositing Manduca sexta

Journal of Chemical Ecology - Many pollinating insects exhibit flower constancy, i.e. they target flower species they have already experienced and fed from. While the insects might profit from...     

The mechanical milling of Al/TiO2 composite powders

The processing of Al/TiO₂ composite powders produced by high-energy mechanical milling leads to production of a range of valuable, titanium-based materials. They include Ti(Al,O)/Al₂O₃ and Ti_xAl_y(O)/Al₂O₃ composite powders, bulk composites and Ti₃Al/TiAl alloy powders, and corresponding bulk materials. The strength of the Ti(Al,O)/Al₂O₃ and Ti_xAl_y(O)/Al₂O₃ composites is moderate, but their high-temperature oxidation resistance is exceptionally high, making the titanium-based composite powders favorable feedstock materials for protective coatings. The hardness of the Ti(Al,O)/Al₂O₃ and Ti₃Al(O)/Al₂O₃ composites is also very high (10–16 GPa). For more information, contact D.L. Zhang, University of Waikato, Waikato Centre for Advanced Materials, Department of Materials and Process Engineering, Private Bag 3105, Hamilton, New Zealand; 011-64-7-838-4783; fax 011-64-7-838-4835; e-mail d.zhang@waikato.ac.nz.     

Numerical simulation of non-linear chatter vibration in turning

In this paper, basic relationships and algorithms for numerical simulation of non-linear, self-excited vibrations in single degree-of-freedom cutting systems are presented. Non-linearities due to the tool leaving the cut, as well as interference between the cutting tool clearance face and cutting surface waviness, were taken into consideration. Examples of vibration simulation results are shown.     

An improved factor based approach to precedential constraint

In this article I argue for rule-based, non-monotonic theories of common law judicial reasoning and improve upon one such theory offered by Horty and Bench-Capon. The improvements reveal some of the interconnections between formal theories of judicial reasoning and traditional issues within jurisprudence regarding the notions of the ratio decidendi and obiter dicta. Though I do not purport to resolve the long-standing jurisprudential issues here, it is beneficial for theorists both of legal philosophy and formalizing legal reasoning to see where the two projects interact.     

Think,feel, bid: the impact of environmental conditions on the role of bidders’ cognitive and affective processes in auction bidding

Environmental conditions and the interplay of cognitive and affective processes both exert influences on bidding behavior. This paper brings the above together, considering how the (external) auction environment determines the impact of (internal) cognitive and affective processes on bidding behavior, assessed in comparison to the optimal bid. Two aspects of the auction environment were considered, namely auction dynamics (low: first-price sealed-bid auction, high: Dutch auction) and value uncertainty (low, high). In a laboratory experiment, we assess bidders’ cognitive workload and emotional arousal through physiological measurements. We find that higher auction dynamics increase the impact of emotional arousal on bid deviations, but not that of cognitive workload. Higher value uncertainty, conversely, increases the impact of cognitive workload on bid deviations, but not that of emotional arousal. Taken together, the auction environment is a critical factor in understanding the nature of the underlying decision process and its impact on bids.     

INSIGMA: an intelligent transportation system for urban mobility enhancement

Intelligent Transportation Systems (ITS) aim to improve safety, mobility and environmental performance of road transport. The INSIGMA project provides a fresh look at the possible innovations in this field, by enhancing the functionality and accuracy of ITS in urban environments. This paper describes the architecture, sensors, processing algorithms, output modules and advantages of the developed system. A comparison of existing ITS systems has been provided as background. Special attention has been given to performance and privacy issues, as the system includes social aspects such as location monitoring.