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71.
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We performed pretreatment brain MRIs in 25 patients with neurologically symptomatic Wilson's disease (WD) and clinical and MRI follow-up in 16 of them. All 25 pretreatment MRIs revealed abnormalities, with abnormal high-signal intensity (HSI) in bilateral thalami being the most common (92%). HSI lesions in the brainstem (84%) and the basal ganglia (72%) were also common. Brain atrophy was present in 88% of the 25 patients. In the follow-up period of 5 to 24 months, during which the patients were treated with D-penicillamine, both HSI lesions and neurologic symptoms improved in 88% of the 16 patients, but the brain atrophy did not change. 相似文献
73.
Recently developed micro- and nano-structured optical fiber sensors, with particular reference to surface plasmon resonance (SPR) fiber sensors and photonic crystal fiber (PCF) sensors are reviewed. SPR fiber sensors can have diverse structures such as D-shape, cladding-off, fiber tip or tapered fiber structures. Some of the recently developed novel structures include the use of various types of fiber gratings in SPR fiber sensors. PCF sensors cover diverse recent developments on photonic-bandgap fiber, holey fiber, hole-assisted fiber and Bragg fiber sensors. Major applications of these include gas sensors and bio-sensors. These micro- and nano-structured fiber sensors have attracted considerable research and development interest, because of their distinct advantages, which include high sensitivity, small sensor head footprint and the flexibility of the optical fibers. They are also of academic interest, and many novel ideas are continuously developed. 相似文献
74.
Chang HW Sung Y Kim KH Nam YD Roh SW Kim MS Jeon CO Bae JW 《Environmental science & technology》2008,42(16):6058-6064
A crucial problem in the use of previously developed genome-probing microarrays (GPM) has been the inability to use uncultivated bacterial genomes to take advantage of the high sensitivity and specificity of GPM in microbial detection and monitoring. We show here a method, digital multiple displacement amplification (MDA), to amplify and analyze various genomes obtained from single uncultivated bacterial cells. We used 15 genomes from key microbes involved in dichloromethane (DCM)-dechlorinating enrichment as microarray probes to uncover the bacterial population dynamics of samples without PCR amplification. Genomic DNA amplified from single cells originating from uncultured bacteria with 80.3-99.4% similarity to 16S rRNA genes of cultivated bacteria. The digital MDA-GPM method successfully monitored the dynamics of DCM-dechlorinating communities from different phases of enrichment status. Without a priori knowledge of microbial diversity, the digital MDA-GPM method could be designed to monitor most microbial populations in a given environmental sample. 相似文献
75.
Analysis of yeast and archaeal population dynamics in kimchi using denaturing gradient gel electrophoresis 总被引:2,自引:0,他引:2
Chang HW Kim KH Nam YD Roh SW Kim MS Jeon CO Oh HM Bae JW 《International journal of food microbiology》2008,126(1-2):159-166
Kimchi is a traditional Korean food that is fermented from vegetables such as Chinese cabbage and radish. Many bacteria are involved in kimchi fermentation and lactic acid bacteria are known to perform significant roles. Although kimchi fermentation presents a range of environmental conditions that could support many different archaea and yeasts, their molecular diversity within this process has not been studied. Here, we use PCR-denaturing gradient gel electrophoresis (DGGE) targeting the 16S and 26S rRNA genes, to characterize bacterial, archaeal and yeast dynamics during various types of kimchi fermentation. The DGGE analysis of archaea expressed a change of DGGE banding patterns during kimchi fermentation, however, no significant change was observed in the yeast DGGE banding patterns during kimchi fermentation. No significant difference was indicated in the archaeal DGGE profile among different types of kimchi. In the case of yeasts, the clusters linked to the manufacturing corporation. Haloarchaea such as Halococcus spp., Natronococcus spp., Natrialba spp. and Haloterrigena spp., were detected as the predominant archaea and Lodderomyces spp., Trichosporon spp., Candida spp., Saccharomyces spp., Pichia spp., Sporisorium spp. and Kluyveromyces spp. were the most common yeasts. 相似文献
76.
77.
78.
Tracking the Fate of Porous Silicon Nanoparticles Delivering a Peptide Payload by Intrinsic Photoluminescence Lifetime 下载免费PDF全文
Yusung Jin Dokyoung Kim Hajung Roh Sojeong Kim Sazid Hussain Jinyoung Kang Chan‐Gi Pack Jun Ki Kim Seung‐Jae Myung Erkki Ruoslahti Michael J. Sailor Song Cheol Kim Jinmyoung Joo 《Advanced materials (Deerfield Beach, Fla.)》2018,30(35)
A nanoparticle system for systemic delivery of therapeutics is described, which incorporates a means of tracking the fate of the nanocarrier and its residual drug payload in vivo by photoluminescence (PL). Porous silicon nanoparticles (PSiNPs) containing the proapoptotic antimicrobial peptide payload, D[KLAKLAK]2, are monitored by measurement of the intrinsic PL intensity and the PL lifetime of the nanoparticles. The PL lifetime of the PSiNPs is on the order of microseconds, substantially longer than the nanosecond lifetimes typically exhibited by conventional fluorescent tags or by autofluorescence from cells and tissues; thus, emission from the nanoparticles is readily discerned in the time‐resolved PL spectrum. It is found that the luminescence lifetime of the PSiNP host decreases as the nanoparticle dissolves in phosphate‐buffered saline solution (37 °C), and this correlates with the extent of release of the peptide payload. The time‐resolved PL measurement allows tracking of the in vivo fate of PSiNPs injected (via tail vein) into mice. Clearance of the nanoparticles through the liver, kidneys, and lungs of the animals is observed. The luminescence lifetime of the PSiNPs decreases with increasing residence time in the mice, providing a measure of half‐life for degradation of the drug nanocarriers. 相似文献
79.
Tae Hwan Park Soon Won Chung Seung Yong Song Dae Hyun Lew Tai Suk Roh Dong Won Lee 《Journal of materials science. Materials in medicine》2018,29(3):27
Although there is ample evidence showing that radiation therapy increases the risk of complications of breast reconstruction, the efficacy of human acellular dermal matrix (CGCryoDerm®) in immediate tissue expander breast reconstruction in the setting of postmastectomy radiation therapy has not been fully elucidated. In this study, we report our institutional experience with pertinent refined surgical technique, and determine whether acellular dermal matrices have a protective effect in this increasingly prevalent clinical setting. Twenty-six patients who underwent immediate two-stage breast reconstruction in the setting of postmastectomy radiation therapy with at least 2 years of follow-up were retrieved. Fifteen patents were reconstructed with ADM, whereas 11 patients were reconstructed without ADM. The occurrence of complications was assessed according to the reconstruction type (with ADM vs without ADM). Furthermore, in patients reconstructed with ADM (n?=?15), immunohistochemistry was performed to analyze the breast capsule with ADM compared with that without ADM in the same patient, according to the expression of alpha-smooth muscle actin (α-SMA). The occurrence of complications was significantly associated with the reconstruction type (with ADM vs. without ADM, p = 0.015). On the basis of the results of α-SMA staining, α-SMA+ myofibroblasts were relatively highly expressed throughout the breast capsule without ADM. On the contrary, α-SMA+ myofibroblasts present at the breast capsule adjacent to the ADM were scarce and irregularly scattered. Use of an acellular dermal matrix may be recommended to patients who are concerned about complications after immediate two-stage breast reconstruction in the setting of postmastectomy radiation therapy. 相似文献
80.
Myoung-hun Han Bong-Soo Roh Kyungwoo Kim Dae-Hoon Kwon Jae-Hyun Ham KyungHyun Yoon Sanghyun Seo 《计算机、材料和连续体(英文)》2022,70(2):2381-2399
An important requirement in a military domain is a highly reliable mobility management method, especially when components of the networks are moving in tactical network environments. To increase reliability, the mobility management technology of the tactical network should be able to reflect the characteristics of the tactical network, such as a limited environment, failure, and hierarchical unit structure. In this paper, we propose a proxy-based hierarchical distributed mobility management scheme, which is highly focused on tactical networks. Considering the characteristics of tactical networks, the proposed scheme is composed of the following: 1) a proxy-based method, 2) a distributed mobility management method that synchronizes a mobility database between entities, and 3) a method of managing mobility by dividing the tactical network into upper and lower layers. Mathematical analysis and modeling and simulation results demonstrate that the method outperforms the existing state-of-the-art method in overcoming entity failure, handover cost, and delay in tactical environments. 相似文献