Parametric-gain approach to the analysis of single-channel DPSK/DQPSK systems with nonlinear phase noise

This paper presents a novel method based on a parametric gain (PG) approach to study the impact of nonlinear phase noise in single-channel dispersion-managed differentially phase-modulated systems. This paper first shows through Monte Carlo simulations that the received amplified spontaneous emission (ASE) noise statistics, before photodetection, can be reasonably assumed to be Gaussian, provided a sufficiently large chromatic dispersion is present in the transmission fiber. This paper then evaluates in a closed form the ASE power spectral density by linearizing the interaction between a signal and a noise in the limit of a distributed system. Even if the received ASE is nonstationary in time due to pulse shape and modulation, this paper shows that it can be approximated by an equivalent stationary process, as if the signal were continuous wave (CW). This paper then applies the CW-equivalent ASE model to bit-error-rate evaluation by using an extension of a known Karhunen-Loe/spl acute/ve method for quadratic detectors in colored Gaussian noise. Such a method avoids calculation of the nonlinear phase statistics and accounts for intersymbol interference due to a nonlinear waveform distortion and optical and electrical postdetection filtering. This paper compares binary and quaternary schemes with both nonreturn- and return-to-zero (RZ) pulses for various values of nonlinear phases and bit rates. The results confirm that PG deeply affects the system performance, especially with RZ pulses and with quaternary schemes. This paper also compares ON-OFF keying (OOK) differential phase-shifted keying (DPSK) systems, showing that the initial 3-dB advantage of DPSK is lost for increasing nonlinear phases because DPSK is less robust to PG than OOK.     

A cytosolic granzyme B inhibitor related to the viral apoptotic regulator cytokine response modifier A is present in cytotoxic lymphocytes

Using a polymerase chain reaction strategy we identified a serine proteinase inhibitor (serpin) in human bone marrow that is related to the cellular serpin proteinase inhibitor 6 (PI-6) and the viral serpin cytokine response modifier A (CrmA). This serpin, proteinase inhibitor 9 (PI-9), has an unusual reactive center P1(Glu)-P1'(Cys), which suggests that it inhibits serine proteinases that cleave after acidic residues. The only known serine proteinase with this specificity is granzyme B, a granule cytotoxin produced by cytotoxic lymphocytes. To test the interaction of PI-9 with granzyme B we prepared recombinant hexa-histidine tagged PI-9 in a yeast expression system. Addition of the recombinant protein to native granzyme B resulted in an SDS-resistant complex typical of serpin-serine proteinase interactions. Further analysis showed that complex formation followed bimolecular kinetics with a second order rate constant of 1.7 +/- 0.3 x 10(6) M-1 s-1, which is in the range for a physiologically significant serpin-proteinase interaction. Recombinant PI-9 also completely abrogated granzyme B and perforin-mediated cytotoxicity in vitro. Examination of PI-9 mRNA distribution demonstrated that it is expressed in immune tissue, primarily in lymphocytes. The highest levels of PI-9 mRNA and protein were observed in natural killer cell leukemia cell lines and in interleukin-2 stimulated peripheral blood mononuclear cells, which also produce granzyme B. Like PI-6, PI-9 was shown to be a cytosolic protein that is not secreted. Fractionation of natural killer cells and stimulated peripheral blood mononuclear cells demonstrated that PI-9 is in a separate subcellular compartment to granzyme B. These results suggest that PI-9 serves to inactivate misdirected granzyme B following cytotoxic cell degranulation. This may explain why cytotoxic cells are not damaged by their own granzyme B during destruction of abnormal cells.     

THE EXACT ERROR IN ESTIMATING THE SPECTRAL DENSITY AT THE ORIGIN

Abstract. This paper derives expressions for the exact bias and variance of a general class of spectral density estimators at the zero frequency, building on the work of Neave (The exact error in spectrum estimates. Ann. Math. Statist. 42 (1971), 961–75) who studied the case where the mean of the series is assumed known. These expressions are evaluated for 15 different windows and for a wide variety of stationary time series. The exact error of the estimators is found to depend on whether the sample mean has to be estimated, and some windows are noticeably inferior at certain values of the bandwidth. A response surface analysis reveals that the finite sample relationships between the bandwidth and the exact error are quite different from the ones suggested by asymptotic theory.     

Propofol analogues. Synthesis, relationships between structure and affinity at GABAA receptor in rat brain, and differential electrophysiological profile at recombinant human GABAA receptors

A number of propofol (2,6-diisopropylphenol) congeners and derivatives were synthesized and their in vitro capability to affect GABAA receptors determined by the inhibition of the specific [35S]-tert-butylbicyclophosphorothionate ([35S]TBPS) binding to rat whole brain membranes. Introduction of halogen (Cl, Br, and I) and benzoyl substituents in the para position of the phenyl group resulted in ligands with higher potency at inhibiting [35S]TBPS binding. A quantitative structure-affinity relationship (QSAR) study demonstrated that affinity is enhanced by increases in lipophilicity of the ligand whereas affinity is adversely affected by increases in size of the substituent para to the phenolic hydroxyl group. Consistent with the displacement of [35S]TBPS and with the activation of GABAA receptors, we demonstrate that ligands displaying high affinity (i.e., 2-4, and 8) are able to increase GABA-stimulated chloride currents in oocytes expressing human GABAA receptors and to directly activate chloride currents in an electrophysiological assay. Among them, compound 4 showed a rather peculiar profile in the electrophysiological examination with cloned alpha1beta2gamma2 GABAA receptors. Indeed, compared to propofol, it displayed a much greater efficacy at potentiating GABA-elicited chloride currents, but a much lower efficacy at producing a direct activation of the chloride channel in the absence of GABA. This behavior may give to compound 4 pharmacological properties that are more similar to anxiolytic and anticonvulsant drugs than to those of general anesthetics.     

Contrast enhancement of LED,vaccum fluorescent and plasma displays with circular polarizing filters

Filters are widely used for contrast enhancement of active electronic displays. This paper considers the application of circular polarizers to LED, vacuum fluorescent and plasma displays under a variety of ambient illumination situations. Relevant parameters controlling contrast enhancement have been identified. The degree of contrast enhancement obtained with appropriate circular polarizing filters under different ambient illumination values is reported for each display type. These values are compared with those obtained using non-polarizing chromatic filters. In general, when contrast is being degraded by specular reflections of ambient light, the circular polarizing filters improve contrast to a much greater extent than the non-polarizing filters.     

Selective regulation of apoptosis: the cytotoxic lymphocyte serpin proteinase inhibitor 9 protects against granzyme B-mediated apoptosis without perturbing the Fas cell death pathway

Cytotoxic lymphocytes (CLs) induce caspase activation and apoptosis of target cells either through Fas activation or through release of granule cytotoxins, particularly granzyme B. CLs themselves resist granule-mediated apoptosis but are eventually cleared via Fas-mediated apoptosis. Here we show that the CL cytoplasmic serpin proteinase inhibitor 9 (PI-9) can protect transfected cells against apoptosis induced by either purified granzyme B and perforin or intact CLs. A PI-9 P1 mutant (Glu to Asp) is a 100-fold-less-efficient granzyme B inhibitor that no longer protects against granzyme B-mediated apoptosis. PI-9 is highly specific for granzyme B because it does not inhibit eight of the nine caspases tested or protect transfected cells against Fas-mediated apoptosis. In contrast, the P1(Asp) mutant is an effective caspase inhibitor that protects against Fas-mediated apoptosis. We propose that PI-9 shields CLs specifically against misdirected granzyme B to prevent autolysis or fratricide, but it does not interfere with homeostatic deletion via Fas-mediated apoptosis.     

Surgical repair of postinfarction left ventricular lesion type I

Left ventricular rupture is the most frequent cause of death following myocardial infarction after ventricular arrhytmias and cardiogenic shock. Under these circumstances, only a prompt diagnosis and urgent surgical treatment can be lifesaving. A review of the literature is made and a simple surgical technique with GRF glue application is presented.     

Use of earthworms (Eisenia fetida) to reduce phytotoxicity and promote humification of pre‐composted olive oil mill wastewater

BACKGROUND: Olive mill wastewaters (OMWW) contain a high recalcitrant organic load and an associated toxicity that make their treatment necessary before environmental application. The aim of this study was to evaluate the feasibility of promoting the valorization and reducing the phytotoxicity of OMWW through a pre‐composting process together with straw‐chip bulking materials followed by the application of earthworms (Eisenia fetida) in the presence of oat seedlings (Avena sativa L.) seedlings. RESULTS: After 3 months, the pre‐composted material showed properties similar to a partially digested compost with some significant amelioration of chemical–physical and biochemical properties. The application of earthworms permitted a significant decrease in chemical (total organic carbon, water‐extractable organic carbon, total nitrogen) and biological parameters (dehydrogenase enzyme activity), and an increase in humic substances and available nitrogen forms. In the presence of plants a higher C/N ratio and a lower content of nitrates were observed. In addition, the reduction in phenolic compounds observed in treatments with earthworms caused a decrease in phytotoxicity by about 50% with respect to the pre‐composted material, which results in an increase in germination index. CONCLUSION: The utilization of earthworms, in particular in the presence of plants, may be an ecologically sound and economically feasible technology to obtain a non‐toxic, high‐value product useful for agricultural purposes. Copyright © 2010 Society of Chemical Industry