Interleukin-12 preserves the graft-versus-leukemia effect of allogeneic CD8 T cells while inhibiting CD4-dependent graft-versus-host disease in mice

We have recently demonstrated that a single injection of 4,900 IU of interleukin-12 (IL-12) on the day of bone marrow transplantation (BMT) markedly inhibits acute graft-versus-host disease (GVHD) in a fully major histocompatibility complex plus minor antigen-mismatched BMT model (A/J --> B10, H-2(a) --> H-2(b)), in which donor CD4(+) T cells are required for the induction of acute GVHD. We show here that donor CD8-dependent graft-versus-leukemia (GVL) effects against EL4 (H-2(b)) leukemia/lymphoma can be preserved while GVHD is inhibited by IL-12 in this model. In mice in which IL-12 mediated a significant protective effect against GVHD, marked GVL effects of allogeneic T cells against EL4 were observed. GVL effects against EL4 depended on CD8-mediated alloreactivity, protection was not observed in recipients of either syngeneic (B10) or CD8-depleted allogeneic spleen cells. Furthermore, we analyzed IL-12-treated recipients of EL4 and A/J spleen cells which survived for more than 100 days. No EL4 cells were detected in these mice by flow cytometry, tissue culture, adoptive transfer, necropsies, or histologic examination. Both GVL effects and the inhibitory effect of IL-12 on GVHD were diminished by neutralizing anti-interferon-gamma (IFN-gamma) monoclonal antibody. This study demonstrates that IL-12-induced IFN-gamma production plays a role in the protective effect of IL-12 against GVHD. Furthermore, IFN-gamma is involved in the GVL effect against EL4 leukemia, demonstrating that protection from CD4-mediated GVHD and CD8-dependent anti-leukemic activity can be provided by a single cytokine, IFN-gamma. These observations may provide the basis for a new approach to inhibiting GVHD while preserving GVL effects of alloreactivity.     

Negative-state relief and the empathy—altruism hypothesis.

Tested the suggestion of R. B. Cialdini et al (see record 1987-21901-001) that the motivation to help associated with empathic emotion is directed toward the egoistic goal of negative-state relief, not toward the altruistic goal of relieving the victim's distress. To test this suggestion, we led empathically aroused Ss to anticipate an imminent mood-enhancing experience. We reasoned that if the motivation to help associated with empathy were directed toward the goal of negative-state relief, then empathically aroused individuals who anticipate mood-enhancement should help less than those who do not. Study 1 verified the effectiveness of our anticipated mood-enhancement manipulation; results indicated that this manipulation could serve as an effective source of negative-state relief. Results of Studies 2 and 3, in which empathy was either measured or manipulated, indicated that the rate of helping among high-empathy Ss was no lower when they anticipated mood enhancement than when they did not. Regardless of anticipated mood enhancement, high-empathy Ss helped more than low-empathy Ss. Results support the empathy–altruism hypothesis. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

On the use of pyrrole black in zinc-halogen batteries

The storage of Br₂/Br^– and I₂/I^– couples in a conducting polymer matrix, polypyrrole coated on a reticulated vitreous carbon disc, is described and the application of these positive electrodes in zinc-halogen model batteries is discussed. The cell based on the polypyrrole bromine adduct shows the higher open circuit voltage which, however, depends on the state of charge. Such cells self discharge thus limiting their usefulness. In the case of the iodine cell the self discharge is due to loss of iodine from the polymer to the bulk solution, but with the bromine cell the cause is oxidative bromination and depolymerization of the polypyrrole.     

A driving-emulation task to study the integration of goals with obligatory and prohibitory traffic signs

This research aims to analyse how drivers integrate the information provided by traffic signs with their general goals (i.e. where they want to go). Some previous studies have evaluated the comparative advantages of obligatory and prohibitory traffic signs using a judgement task. In this work, a new experimental task with greater similarity to driving situations is proposed. Participants imagine they are driving a vehicle and must make right or left turn manoeuvres according to a previously indicated objective and the information from obligatory and prohibitory traffic signs. Eighty-two participants took part in two different experiments. According to the results, an obligatory traffic sign is associated with faster and more accurate responses only when the participant's initial objective is allowed. When the initial objective was not allowed, an advantage in accuracy was observed with prohibitory traffic signs and there was no significant difference in reaction time between the two types of sign. These results suggest that having an obligatory traffic sign may facilitate a correct response when the driver's goal is effectively allowed, whereas a prohibitory traffic sign could be more effective in preventing error when the driver has a not-allowed goal in mind. However, processing a prohibitory sign requires an extra inference (i.e. deciding which is the allowed manoeuvre), and thus the potential advantage in reaction time of the prohibitory sign may disappear. A second experiment showed that the results could not be explained by a potential congruency effect between the location (left or right) of the road signs and the position of the key or the hand used to respond (such as the Simon effect or the spatial Stroop effect). Also, an increase in the difficulty of the task (using an incongruent hand to respond) affected performance more strongly in experimental conditions that required making inferences. This made the advantage of the obligatory sign over the prohibitory sign in this condition more noteworthy. The evidence gathered in the current study could be of particular interest in some applied research areas, such as the assessment of road traffic signalling strategies or the ergonomic design of GPS navigation systems.     

Many Distances in Planar Graphs

We show how to compute in O(n ^4/3log?^1/3 n+n ^2/3 k ^2/3log?n) time the distance between k given pairs of vertices of a planar graph G with n vertices. This improves previous results whenever (n/log?n)^5/6≤k≤n ²/log?⁶ n. As an application, we speed up previous algorithms for computing the dilation of geometric planar graphs.     

Introduction to the special issue of the ECML PKDD 2021 journal track

Data Mining and Knowledge Discovery -     

A proposal for statistical evaluation of the detection of gunshot residues on a suspect

The possibility of accidental contamination of a suspect by gunshot residues (GSRs) is considered. If two hypotheses are taken into account ("the suspect has shot a firearm" and "the suspect has not shot a firearm"), the likelihood ratio of the conditional probabilities of finding a number n of GSRs is defined. Choosing two Poisson distributions, the parameter lambda of the first one coincides with the mean number of GSRs that can be found on a firearm shooter, while the parameter mu of the second one is the mean number of GSRs that can be found on a nonshooter. In this scenario, the likelihood ratio of the conditional probabilities of finding a number n of GSRs in the two hypotheses can be easily calculated. The evaluation of the two parameters lambda and mu and of the goodness of the two probability distributions is performed by using different sets of data: "exclusive" lead-antimony-barium GSRs have been detected in two populations of 31 and 28 police officers at diverse fixed times since firearm practice, and in a population of 81 police officers who stated that they had not handled firearms for almost 1 month. The results show that the Poisson distributions well fit the data for both shooters and nonshooters, and that the probability of detection of two or more GSRs is normally greater if the suspect has shot firearms.     

Synchronous condition-based consensus

The condition-based approach studies restrictions on the inputs to a distributed problem, called conditions, that facilitate its solution. Previous work considered mostly the asynchronous model of computation. This paper studies conditions for consensus in a synchronous system where processes can fail by crashing. It describes a full classification of conditions for consensus, establishing a continuum between the asynchronous and synchronous models, with the following hierarchy where includes all conditions (and in particular the trivial one made up of all possible input vectors). For a condition , we have:

Spatial Optimization Models for Water Supply Allocation

Climate change is likely to result in increased aridity, lower runoff, and declining water supplies for the cities of the Southwestern United States, including Phoenix. The situation in Phoenix is particularly complicated by the large number of water providers, each with its own supply portfolio, demand conditions, and conservation strategies. This paper details spatial optimization models to support water supply allocation between service provider districts, where some districts experience deficits and others experience surpluses in certain years. The approach seeks to reconcile and integrate projections derived from a complex simulation model taking into account current and future climate conditions. The formulated and applied models are designed to help better understand the expected increasingly complex interactions of providers under conditions of climate change. Preliminary results show cooperative agreements would reduce spot shortages that would occur even without climate change. In addition, they would substantially reduce deficits if climate change were to moderately reduce river flows in Phoenix’s major source regions, but have little effect under the most pessimistic scenarios because there are few surpluses available for re-allocation.