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101.
The flow downstream of a pair of circular cylinder in a side-by-side arrangement normal to the free stream is known to exhibit intermittently bistable structure for the range of G/D = 1.2–2.2 where G is the center-to-center distance between the cylinders and D is the diameter of the cylinder. Eventually, the wake downstream of one of the two cylinders can be wider or narrower than the one downstream of the other cylinder depending on the direction of gap-flow deflection. In the present study, such an asymmetric flow behavior downstream of two side-by-side cylinders, which were vertically located in shallow water, was passively controlled with a splitter plate with a length of L   (1?L/D?51?L/D?5). The center of splitter plate was just coincided with the mid-height of the gap between centers of the cylinders. The investigations were carried out in a water channel using dye visualization and particle image velocimetry, PIV for qualitative and quantitative measurements, respectively. The diameter of the cylinder, D was 40 mm while the depth of water was 20 mm so that the shallow flow condition was provided through the experiments. The Reynolds number, Re based on D was 5000 and the cylinder’s center to center spacing to the cylinder diameter ratio (G/D  ) was equal to 1.25. The results demonstrated that the deflection of the wake and thereby the bistability of the wake was considerably prevented with the presence of the splitter plate for L/D?3L/D?3 which resulted in two well symmetric, stable wakes having approximately the same order of magnitudes of vortex shedding frequencies around the cylinders.  相似文献   
102.
Identification of meat species by TaqMan-based real-time PCR assay   总被引:5,自引:0,他引:5  
In this study, a convenient, sensitive and specific real-time PCR assay was described for the species identification and their quantification in raw and cooked meat products. Specific primers and TaqMan probes were designed on the mitochondrial ND2, ND5 and ATP 6-8 genes for donkey, pork and horse, respectively, and the performance of the method was tested. In the results, no cross-reaction was observed between the donkey and pork species specific primer-probe systems and non-target species (bovine, ovine, chicken and turkey). Only one cross reaction was observed between the horse species specific primer-probe set and 100 ng pork DNA at the ct 33.01 level (corresponding to 0.01 ng horse DNA). The real-time quantitative assay used in this study allowed the detection of as little as 0.0001 ng template DNA from pure meat for each species investigated and experimental meat mixtures. In conclusion, it can be suggested that the TaqMan probe assay used in this research might be a rapid and sensitive method for the routine meat species identifications studies in raw or cooked meat products.  相似文献   
103.
The characterizations of Co-Fe films electrodeposited on Ti substrates under potentiostatic conditions were investigated as a function of the Fe content in the films. The compositional analysis was carried out by energy dispersive X-ray spectroscopy. Scanning electron microscopy used to analyze the surface morphology of the films revealed that the film surface became rather smooth with the increase of the Fe contents. X-ray diffraction patterns showed that the crystal structure changed depending on the Co:Fe ratio in the films. It was observed that the crystal structure converted from the predominant face-centered-cubic to the body-centered-cubic with increasing Fe content. All films showed anisotropic magnetic resistance, but their magnitudes decrease as the Fe content increases. Magnetic data obtained from by vibrating sample magnetometer revealed that the changes observed in the saturation magnetization and coercivity values may arise from the Fe content of the films. The different magnetic and magnetotransport properties may come from the structural differences caused by the Fe content.  相似文献   
104.
At present, Pd containing (10–40 wt%) multiwall carbon nanotube (MWCNT) supported Pd monometallic, Pd:Au bimetallic, and PdAuCo trimetallic catalysts are prepared via NaBH4 reduction method to examine their formic acid electrooxidation activities and direct formic acid fuel cell performances (DFAFCs) when used as anode catalysts. These catalysts are characterized by advanced analytical techniques as N2 adsorption and desorption, XRD, SAXS, SEM-EDX, and TEM. Electronic state of Pd changes by the addition of Au and Co. Moreover, formic acid electrooxidation activities of these catalysts measured by CV indicates that particle size changes in wide range play a major role in the formic acid electrochemical oxidation activity, ascribed the strong structure sensitivity of formic acid electrooxidation reaction. PdAuCo (80:10:10)/MWCNT catalyst displays the most significant current density increase. On the other hand, lower CO stripping peak potential obtained for PdAuCo (80:10:10)/MWCNT catalyst, attributed to the awakening of the Pd-adsorbate bond strength down to its optimum value, which favors higher electrochemical activity. DFAFCs performance tests and exergy analysis reveal that fuel cell performances increase with the addition of Au and Co which can be attributed to synergetic effect. Furthermore, temperature strongly influences the performance of formic acid fuel cell.  相似文献   
105.
Indoor radon activity level and radon effective dose (ED) rate have been carried out in the rural dwellings of Ezine (Canakkale) during the summer season using Radosys-2000, a complete set suitable to radon concentration measurements with CR-39 plastic alpha track detectors. The range of radon concentration varied between 9 and 300 Bq m(-3), with an average of 67.9 (39.9 SD) Bq m(-3). Assuming an indoor occupancy factor of 0.8 and 0.4 for the equilibrium factor of radon indoors, it has been found that the 222Rn ED rate in the dwellings studied ranges from 0.4 to 5.2 mSv y(-1), with an average value of 1.7 (1.0) mSv y(-1). There is a possibility that low radon concentrations exist indoors during the summer season in the study area because of relatively high ventilation rates in the dwellings. A winter survey will be needed for future estimation of the annual ED.  相似文献   
106.
There is a strong demand for portable systems that can detect and characterize individual pathogens and other nanoscale objects without the use of labels, for applications in human health, homeland security, environmental monitoring and diagnostics. However, most nanoscale objects of interest have low polarizabilities due to their small size and low refractive index contrast with the surrounding medium. This leads to weak light-matter interactions, and thus makes the label-free detection of single nanoparticles very difficult. Micro- and nano-photonic devices have emerged as highly sensitive platforms for such applications, because the combination of high quality factor Q and small mode volume V leads to significantly enhanced light-matter interactions. For example, whispering gallery mode microresonators have been used to detect and characterize single influenza virions and polystyrene nanoparticles with a radius of 30 nm (ref. 12) by measuring in the transmission spectrum either the resonance shift or mode splitting induced by the nanoscale objects. Increasing Q leads to a narrower resonance linewidth, which makes it possible to resolve smaller changes in the transmission spectrum, and thus leads to improved performance. Here, we report a whispering gallery mode microlaser-based real-time and label-free detection method that can detect individual 15-nm-radius polystyrene nanoparticles, 10-nm gold nanoparticles and influenza A virions in air, and 30 nm polystyrene nanoparticles in water. Our approach relies on measuring changes in the beat note that is produced when an ultra-narrow emission line from a whispering gallery mode microlaser is split into two modes by a nanoscale object, and these two modes then interfere. The ultimate detection limit is set by the laser linewidth, which can be made much narrower than the resonance linewidth of any passive resonator. This means that microlaser sensors have the potential to detect objects that are too small to be detected by passive resonator sensors.  相似文献   
107.
The effects of fat content and emulsifier type on the rheological properties of cake batter have been investigated by using a parallel-plate rheometer. The apparent viscosity of cake batter with five different fat concentrations (0, 12.5, 25, 37.5, and 50%) and two types of emulsifier, namely Purawave and Lecigran, was studied as a function of the shear rate. In addition, the time dependency of different cake formulations was investigated. It was found that cake batter with different fat concentrations and emulsifier types exhibited shear thinning and time-independent behavior. Experimental data provided a good fit for the power law model. The increase in fat content and addition of emulsifier caused a decrease in the apparent viscosity. The flow behavior index was not found to be dependent on the composition of cake batter.  相似文献   
108.
The effects of batter coating containing pregelatinized tapioca starch or maltodextrin at different concentrations (1, 3 or 5%) on product quality of deep-fat-fried carrot slices were evaluated. Coated slices were fried for 2, 3 and 4 min at 170 °C. Coating pickup of batter formulations and moisture and oil contents, porosity, texture and colour of fried slices were determined. Batter without pregelatinized starch or maltodextrin addition was used as the control. Addition of 5% pregelatinized tapioca starch to the batter formulation provided the crispiest product with the lowest oil content. Increasing maltodextrin concentrations enhanced the crispness and colour development of the fried product but had an adverse effect on porosity, moisture and oil contents of carrot slices.  相似文献   
109.
The presence and species diversity of staphylococci in 250 ground beef and lamb meat samples obtained from Diyarbakir, Turkey were investigated. The presence of the 16S rRNA gene, mecA, nuc, pvl, and femA was analyzed by multiplex PCR. Pheno‐ and genotypic antibiotic resistance profiles of 208 staphylococci isolates were established. Of the ground beef and ground lamb samples, 86.4% and 62.4% were positive for staphylococci, respectively. Staphylococcus aureus, S. saprophyticus, S. hominis, S. lentus, S. pasteuri, S. warneri, S. intermedius, and S. vitulinus made up 40.8%, 28.8%, 11%, 3.8%, 3.8%, 2.4%, 2.4%, and 2.4% of isolates, respectively. Of the 85 S. aureus isolates, 40%, 47%, and 5.8% carried femA, mecA, and pvl, respectively, whereas the corresponding rates for the 118 coagulase‐negative staphylococci (CoNS) were 0%, 10.1%, and 0%, respectively. We determined from the 208 isolates, the highest antibiotic resistances were to tetracycline and oxytetracycline (85.5%), followed by penicillin (51.4%), novobiocin (45.6%), ampicillin (39.9%), and doxycycline (31.7%), using the Clinical and Laboratory Standards Inst. (CLSI) method. All isolates were sensitive to gentamycin, ofloxacin, and tobramycin, but 2.3% of the S. aureus isolates had resistance to vancomycin. The staphylococci isolates carried tet(K), blaZ, tet(L), tet(W), cat, tet(S), tet(M), ermB, ermA, and ermC antibiotic resistance genes at rates of 59%, 51.7%, 36.9%, 31.8%, 27.2%, 27.2%, 24.4%, 18.1%, 7.9%, and 3.9%, respectively.  相似文献   
110.
Microwave heating can be combined with other means of heating to yield a unique heating profile. In the study, microwave frying, a combination of convective and microwave heating, was compared with conventional frying. Frying experiments were performed by inserting a single food sample (chicken breast meat) in the hot oil at 180?±?1°C for both frying methods. Center temperature of the sample and the oil temperature were recorded during both frying methods. Simulations were performed to predict heat transfer coefficients. Processing time was shorter with microwave frying. Simulations revealed a varying convective heat transfer coefficient, which was in the range of 160–490 W/m2 K, during conventional frying. Higher convective heat transfer coefficient, 500 W/m2 K, compared to conventional frying was observed during microwave frying with the simulations. This is suggested to be due to higher turbulence in microwave frying.  相似文献   
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