全文获取类型
收费全文 | 4217篇 |
免费 | 468篇 |
国内免费 | 10篇 |
专业分类
电工技术 | 42篇 |
综合类 | 1篇 |
化学工业 | 1145篇 |
金属工艺 | 111篇 |
机械仪表 | 218篇 |
建筑科学 | 105篇 |
矿业工程 | 1篇 |
能源动力 | 176篇 |
轻工业 | 350篇 |
水利工程 | 10篇 |
石油天然气 | 2篇 |
武器工业 | 2篇 |
无线电 | 936篇 |
一般工业技术 | 1039篇 |
冶金工业 | 173篇 |
原子能技术 | 28篇 |
自动化技术 | 356篇 |
出版年
2024年 | 2篇 |
2023年 | 62篇 |
2022年 | 67篇 |
2021年 | 140篇 |
2020年 | 119篇 |
2019年 | 134篇 |
2018年 | 176篇 |
2017年 | 151篇 |
2016年 | 172篇 |
2015年 | 149篇 |
2014年 | 228篇 |
2013年 | 298篇 |
2012年 | 339篇 |
2011年 | 351篇 |
2010年 | 268篇 |
2009年 | 253篇 |
2008年 | 283篇 |
2007年 | 199篇 |
2006年 | 155篇 |
2005年 | 135篇 |
2004年 | 134篇 |
2003年 | 122篇 |
2002年 | 110篇 |
2001年 | 101篇 |
2000年 | 83篇 |
1999年 | 82篇 |
1998年 | 101篇 |
1997年 | 60篇 |
1996年 | 41篇 |
1995年 | 43篇 |
1994年 | 25篇 |
1993年 | 16篇 |
1992年 | 11篇 |
1991年 | 6篇 |
1990年 | 14篇 |
1989年 | 11篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1976年 | 3篇 |
1975年 | 1篇 |
1973年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
排序方式: 共有4695条查询结果,搜索用时 15 毫秒
31.
Dinesh Kalyanasundaram Shinnosuke Inoue Jong-Hoon Kim Hyun-Boo Lee Zenko Kawabata Woon-Hong Yeo Gerard A. Cangelosi Kieseok Oh Dayong Gao Kyong-Hoon Lee Jae-Hyun Chung 《Microfluidics and nanofluidics》2012,13(2):217-225
Simple, high-yield concentration of DNA is important for high-throughput genetic analysis and disease diagnosis. Glass-based microfilters are popular but the process requires centrifugation steps with cumbersome chemical processes. As an alternative, a concentration method using an electric field has been explored previously, but with limited efficiency. In this paper, electric field-induced concentration and capture of DNA are studied by using high-aspect-ratio microtips coated with a gold layer. The microtips are immersed longitudinally into a solution of 100???L containing ??-phage DNA. After DNA concentration using an electric field, the microtips are withdrawn from the solution. Under AC- and biased AC fields, DNA is concentrated by electrophoresis (EP), dielectrophoresis (DEP), and electroosmotic flow (EOF). To reduce capillary effects in the withdrawal process, the microtips are coated with positively charged poly-l-lysine (PLL). The pattern of captured DNA is analyzed by fluorescence microscopy. DEP attracts DNA molecules at the edges of microtips, where the highest gradient of electric field exists. EP attracts DNA onto the surface of microtips following the vectors of an electric field. EOF generates vortexes that deliver DNA onto microtips. Using this method, 85% of DNA is captured on the PLL-coated microtips after three sequential captures. The concentration mechanism can potentially facilitate rapid and simple preparation of DNA for downstream analysis. 相似文献
32.
We report an active micromixer utilizing vortex generation due to non-equilibrium electrokinetics near micro/nanochannel interfaces.
Its design is relatively simple, consisting of a U-shaped microchannel and a set of nanochannels. We fabricated the micromixer
just using a two-step reactive ion etching process. We observed strong vortex generation in fluorescent microscopy experiments.
The mixing performance was evident in a combined pressure-driven and electroosmotic flows, compared with the case with a pure
pressure-driven flow. We characterized the micromixer for several conditions: different applied voltages, ion concentrations,
flow rates, and nanochannel widths. The experimental results show that the mixing performance is better with a higher applied
voltage, a lower ion concentration, and a wider nanochannel width. We quantified the mixing characteristics in terms of mixing
time. The lowest mixing time was 2 milliseconds with the voltage of 230 V and potassium chloride solutions of 0.1 mM. We expect
that the micromixer is beneficial in several applications requiring rapid mixing. 相似文献
33.
Eugene Choi Sung Jean Park Gunhee Lee Seung Kew Yoon Minho Lee Suk Kyeong Lee 《International journal of molecular sciences》2021,22(6)
Hepatocellular carcinoma (HCC), the most common malignant tumor in the liver, grows and metastasizes rapidly. Despite advances in treatment modalities, the five-year survival rate of HCC remains less than 30%. We sought genetic mutations that may affect the oncogenic properties of HCC, using The Cancer Genome Atlas (TCGA) data analysis. We found that the GNAQ T96S mutation (threonine 96 to serine alteration of the Gαq protein) was present in 12 out of 373 HCC patients (3.2%). To examine the effect of the GNAQ T96S mutation on HCC, we transfected the SK-Hep-1 cell line with the wild-type or the mutant GNAQ T96S expression vector. Transfection with the wild-type GNAQ expression vector enhanced anchorage-independent growth, migration, and the MAPK pathways in the SK-Hep-1 cells compared to control vector transfection. Moreover, cell proliferation, anchorage-independent growth, migration, and the MAPK pathways were further enhanced in the SK-Hep-1 cells transfected with the GNAQ T96S expression vector compared to the wild-type GNAQ-transfected cells. In silico structural analysis shows that the substitution of the GNAQ amino acid threonine 96 with a serine may destabilize the interaction between the regulator of G protein signaling (RGS) protein and GNAQ. This may reduce the inhibitory effect of RGS on GNAQ signaling, enhancing the GNAQ signaling pathway. Single nucleotide polymorphism (SNP) genotyping analysis for Korean HCC patients shows that the GNAQ T96S mutation was found in only one of the 456 patients (0.22%). Our data suggest that the GNAQ T96S hotspot mutation may play an oncogenic role in HCC by potentiating the GNAQ signal transduction pathway. 相似文献
34.
35.
36.
Arun Pandian Chandrasekaran Sang Hyeon Woo Neha Sarodaya Byung Ho Rhie Apoorvi Tyagi Soumyadip Das Bharathi Suresh Na Re Ko Seung Jun Oh Kye-Seong Kim Suresh Ramakrishna 《International journal of molecular sciences》2021,22(11)
Cell division cycle 25A (Cdc25A) is a dual-specificity phosphatase that is overexpressed in several cancer cells and promotes tumorigenesis. In normal cells, Cdc25A expression is regulated tightly, but the changes in expression patterns in cancer cells that lead to tumorigenesis are unknown. In this study, we showed that ubiquitin-specific protease 29 (USP29) stabilized Cdc25A protein expression in cancer cell lines by protecting it from ubiquitin-mediated proteasomal degradation. The presence of USP29 effectively blocked polyubiquitination of Cdc25A and extended its half-life. CRISPR-Cas9-mediated knockdown of USP29 in HeLa cells resulted in cell cycle arrest at the G0/G1 phase. We also showed that USP29 knockdown hampered Cdc25A-mediated cell proliferation, migration, and invasion of cancer cells in vitro. Moreover, NSG nude mice transplanted with USP29-depleted cells significantly reduced the size of the tumors, whereas the reconstitution of Cdc25A in USP29-depleted cells significantly increased the tumor size. Altogether, our results implied that USP29 promoted cell cycle progression and oncogenic transformation by regulating protein turnover of Cdc25A. 相似文献
37.
Seung Beom Cho Sridhar Venigalla James H. Adair † 《Journal of the American Ceramic Society》1996,79(1):88-96
Phase-pure, monodispersed, hexagonal plates of single-crystal α-alumina (∼ 2 μm wide and ∼0.5 μm thick) have been prepared via precipitation by treating an aluminum hydrous oxide precursor in 1,4-butanediol at 300°C under autogenous vapor pressure. Present work shows that KOH is the only reagent that precipitates an aluminum hydrous oxide precursor suitable to synthesize α-alumina in 1,4-butanediol solution. In contrast, the use of NaOH or NH4 OH as the precipitating reagent for the precursor material does not yield the alpha phase. The solution pH at which the precursor materials are precipitated is also a critical factor for the formation of α-Al2 O3 . Phase-pure α-alumina powders were also only synthesized from the aluminum hydrous oxide precursors precipitated in the pH range from 10 to 10.5. The results of X-ray diffraction and scanning electron microscopy indicate that longer reaction times promote the phase transformation from the intermediate boehmite phase to α-alumina. The complete transformation from boehmite to α-alumina requires reaction times of about 12 h. 相似文献
38.
By measuring Tg, Tm and Tc (cloud point) phase diagrams for the four miscible blends of chlorinated isotactic polypropylenes (chlorine content 39.2 (CPP-40) and 49.8 wt.-% (CP-50)) with Poly(ethylene-co-vinyl acetate)s (vinyl acetate contents 40 (EVA-40) and 45 wt.-% (EVA-45)) were investigated. The blend of CPP-50 with EVA-40 was the most compatible of the four blend pairs. 相似文献
39.
Electrospinning of poly(lactic‐co‐glycolic acid) (PLGA) in chloroform or 1,1,1,3,3,3‐hexafluoro‐2‐propanol (HFIP) was investigated, focusing on its solution parameters, to develop nonwoven biodegradable nanofibrous structures for tissue engineering. PLGA nanofibers were obtained by electrospinning of 15 wt % PLGA solution and the resulting average fiber diameters were varied with the range of 270–760 nm, depending on solution property. When small amounts of benzyl triethylammonium chloride (BTEAC) was added to the PLGA/chloroform solution, the average diameter was decreased from 760 to 450 nm and the fibers were densely amounted in a straight shape. In addition, the average fiber diameter (270 nm) of nanofibers electrospun from polar HFIP solvent was much smaller than that (760 nm) of nanofibers electrospun from nonpolar chloroform solvent. Therefore, it could be concluded that conductivity or dielectric constant of the PLGA solution was a major parameter affecting the morphology and diameter of the electrospun PLGA fibers. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 99: 1214–1221, 2006 相似文献
40.
Natural manganese ores were selected as raw materials for the desulfurization sorbent because of economical efficiency and high reactivity on hydrogen sulfide. Initial reaction rates between H2S and desulfurization sorbent of natural manganese ores were determined in a temperature range of 400-800°C using a thermobalance reactor. All reactions were first order with respect to H2S and were expressed by the Arrhenius relation. When the sulfidation reaction was controlled by diffusion, the temperature dependence of the effective diffusivity was given by the Arrhenius equation. Activation energies and frequency factors were obtained from the product layer diffusion coefficient of various sorbents by plotting as an Arrhenius equation form. Several additives were mixed to improve the sulfidation capacity, and NiO was the best additive. 相似文献