Inhibition of intratracheal lung cancer development by systemic delivery of E1A

Amplification or overexpression of HER-2/neu in human lung cancer has been correlated with poor prognosis and chemoresistance. We have previously reported that the adenovirus type 5 early region 1A (E1A) gene product can suppress HER-2/neu-mediated transformation phenotypes through inhibition of HER-2/neu expression. To find an efficient way to treat HER-2/neu-overexpressing lung cancer with E1A, a replication-deficient adenovirus containing the E1A gene, Ad.E1A(+), was used to transduce E1A into HER-2/neu-overexpressing and low expressing human lung cancer cell lines. Tumour cell growth in vitro and colony formation in soft agarose were greatly inhibited by Ad.E1A(+) transduction in HER-2/neu-overexpressing lung cancer cell lines. In HER-2/neu low expressing cell lines, E1A could not inhibit cell growth in vitro but could reduce the colony formation ability in soft agarose, indicating different effects of E1A in these two types of cancer cells. To test the therapeutic efficacy of E1A to lung cancer by systemic delivery in vivo, tumor-bearing mice were established by intratracheal injection of lung cancer cells and treated by i.v. tail injections of Ad.E1A(+). As a result, Ad.E1A(+) suppressed HER-2/neu overexpression and inhibited intratracheal lung cancer growth. However, no significant tumor suppression effect of Ad.E1A(+) was observed in mice bearing HER-2/neu low expressing cell line when the same therapeutic procedure was followed. Thus, we conclude that systemic delivery of Ad.E1A(+) can efficiently achieve therapeutic effect in HER-2/neu-overexpressing lung cancer in vivo.     

广钢转炉分厂2号方坯连铸机拉速问题的探讨

通过分析钢水在结晶器内凝固的不均匀性及对钢水结晶的热量平衡计算，讨论连铸中间包钢水温度、结晶器冷却水流量及进出水温差对拉坯速度的影响。     

任丘油田雾迷山油藏抽油机井系统效率初步探讨

总公司1996年对任丘雾迷山油藏抽油机井进行测试,系统效率高达51%~68%,其结果出乎意料。从影响抽油机系统效率的几大因素入手,分析出任丘雾迷山油藏抽油机井系统效率高的主要原因。任丘雾迷山油藏属裂缝性破酸盐岩油藏(即灰岩油战),由于其裂缝发育、渗透性好、油层厚度大、地层温度高、油气比小、饱和压力低等,使得抽油机井生产呈现如下特点:()大泵径,长冲程,低冲次大排量采油;(2)中浅京挂,小沉没度;(3)抽汲流体为液体单相,泵充满程度高;(4)部分井为连抽"带喷"状态,抽油系活塞底部受力大;(5)井液粘度低,油井不结蜡,摩阻损失小。由于这些特点以及近年来使用的新型节能抽油机,使得抽油机井系统效率大大高于其它油田抽油机井系统效率。     

费萨姆工艺无水氨精馏塔的模拟计算

本文以ＮＨ３－Ｈ２Ｏ系统Ｇｉｂｂｓ自由能、熵、焓和汽液相平衡的热力学模型为基础，用松弛法进行了无水氨精馏塔的模拟计算。改变无水氨采出比、回流比（直汽量）和进料板位置均影响蒸馏效果。     

L－B膜修饰电极循环伏安行为模拟──（Ⅰ）电荷转移速度常数对循环伏安性能的影响

本文用计算机数字模拟方法研究了电活性分子多层Ｚ型Ｌ－Ｂ膜修饰电极的循环伏安行为。计算了电极与修饰Ｌ－Ｂ膜分子第一层之间的电荷转移速度常数Ｋ_ｏ，Ｌ－Ｂ膜分子层间的电荷转移速度常数ｋ_ｉ；对峰电位差△Ｅ_ｐ及阳极峰面积Ｑ的影响，以及在不同条件下各层分子的氧化态分数随扫描时间的变化。为研究和设计电活性分子修饰电极的实际体系提供了大量数据和信息。     

工业锅炉群集散控制系统

介绍了XMC-3000工业热水锅炉群集散控制系统的硬件组成及特点,描述了锅炉燃烧系统、定压系统的控制方案和系统供热调节模型,最后给出了系统的主要功能。     

DG130/3.82-4型自然循环锅炉水冷壁管爆管技术分析

对水冷壁管同一根、同一区域发生的两起爆管事故进行失效分析 ,研究爆管机理 ,找出了爆管原因 ,提出了预防措施     

A multi-agent decision support system for stock trading

A distributed problem solving system can be characterized as a group of individual cooperating agents running to solve common problems. As dynamic application domains continue to grow in scale and complexity, it becomes more difficult to control the purposeful behavior of agents, especially when unexpected events may occur. This article presents an information and knowledge exchange framework to support distributed problem solving. From the application viewpoint the article concentrates on the stock trading domain; however, many presented solutions can be extended to other dynamic domains. It addresses two important issues: how individual agents should be interconnected so that their resources are efficiently used and their goals accomplished effectively; and how information and knowledge transfer should take place among the agents to allow them to respond successfully to user requests and unexpected external situations. The article introduces an architecture, the MASST system architecture, which supports dynamic information and knowledge exchange among the cooperating agents. The architecture uses a dynamic blackboard as an interagent communication paradigm to facilitate factual data, business rule, and command exchange between cooperating MASST agents. The critical components of the MASST architecture have been implemented and tested in the stock trading domain, and have proven to be a viable solution for distributed problem solving based on cooperating agents     

铜铅锌银多金属矿湿法分离新工艺

依据不同金属硫化物氧化分解电位的差异 ,针对某含铜、铅、锌、银复杂多金属硫化矿的难选冶特性 ,对浮选获得的混合精矿采取在线控制浸出过程氧化还原电位的方法 ,分步选择性氯化浸出各有价元素 ,并辅之以沉淀、置换、萃取等工艺 ,使有价金属以较高的回收率得到了有效的分离提取 ,在各工序最佳条件下 ,Cu、Pb、Zn、Ag各金属的浸出率均大于 99 0 % ,元素硫的回收率大于 76%     

10万t/a甲醇装置的设计及变更实践

介绍了10万t/a甲醇装置设计与变更的指导思想，变更内容及实际运行情况。实践证明，变更后工艺流程更加合理，各项消耗指标优于设计值。