Apparatus for measuring physical adsorption on intact supported porous membranes

We present a non-destructive, reusable apparatus with which to measure physical adsorption isotherms of intact porous membranes. We developed a two-piece sample container to make reliable sorption measurements of porous membranes, the properties of which may differ from those of powders due to crystal intergrowth. This novel system employs a resealable container that can be immersed in a cryogenic bath, into which tubular, planar, or other porous membranes and films may be placed. Detailed sorption isotherms, including high-resolution adsorption in the low pressure (micropore-filling) region, are shown for MFI-type zeolite membranes grown on two types and configurations of α-alumina support.     

Fatty acid composition of muscle fat and enzymes of storage lipid synthesis in whole muscle from beef cattle

Enhanced intramuscular fat content (i.e., marbling) in beef is a desirable trait, which can result in increased product value. This study was undertaken with the aim of revealing biochemical factors associated with the marbling trait in beef cattle. Samples of longissimus lumborum (LL) and pars costalis diaphragmatis (PCD) were taken from a group of intact crossbred males and females at slaughter, lipids extracted, and the resulting FAME examined for relationships with marbling fat deposition. For LL, significant associations were found between degree of marbling and myristic (14∶0, r=0.55, P<0.01), palmitic (16∶0, r=0.80 P<0.001), stearic (18∶0, r=−0.58, P<0.01), and oleic (18∶1c-9, r=0.79, P<0.001) acids. For PCD, significant relationships were found between marbling and palmitic (r=0.71, P<0.001) and oleic (r=0.74, P<0.001) acids. Microsomal fractions prepared from PCD muscle were assayed for diacylglycerol acyltransferase (DGAT), lysophosphatidic acid acyltransferase (LPAAT), and phosphatidic acid phosphatase-1 (PAP-1) activity, and the results examined for relationships with degree of intramuscular fat deposition. None of the enzyme activities from PCD displayed an association with marbling fat content, but DGAT specific activity showed significant positive associations with LPAAT (r=0.54, P<0.01), total PAP (r=0.66, P<0.001), and PAP-1 (r=0.63, P<0.01) specific activities. The results on FA compositions of whole muscle tissues provide insight into possible enzyme action associated with the production of specific FA. The increased proportion of oleic acid associated with enhanced lipid content of whole muscle is noteworthy given the known health benefits of this FA.     

Tetracycline resistance genes in activated sludge wastewater treatment plants

The development and proliferation of antibiotic resistance in pathogenic, commensal, and environmental microorganisms is a major public health concern. The extent to which human activities contribute to the maintenance of environmental reservoirs of antibiotic resistance is poorly understood. In the current study, wastewater treatment plants (WWTPs) were investigated as possible sources of tetracycline resistance via qualitative PCR and quantitative PCR (qPCR). Various WWTPs and two freshwater lakes were surveyed for the presence of an array of 10 tetracycline resistance determinants (tet(R)): tet(A)-(E), tet(G), tet(M), tet(O), tet(Q), tet(S). All WWTP samples contained more different types of tet(R) genes, as compared to the lake water samples. Gene copy numbers of tet(G) and tet(Q) in these samples were quantified via qPCR and normalized to both the volume of original sample and to the amount of DNA extracted per sample (a proxy for bacterial abundance). Concentrations of tet(Q) were found to be highest in wastewater influent while tet(G) concentrations were highest in activated sludge. Investigation of the effects of UV disinfection on wastewater effluent showed no reduction in the number of detectable tet(R) gene types.     

Iron oxide amended biosand filters for virus removal

Laboratory studies were performed to determine if the addition of iron oxides throughout biosand filter (BSF) media would increase virus removal due to adsorption. The proposed mechanism is electrostatic adsorption of negatively charged virion particles to positively charged iron oxides formed during the corrosion of zerovalent iron. Initial tests conducted using continuous flow, small-scale glass columns showed high MS2 bacteriophage removal in an iron-amended sand column (5log₁₀) compared to a sand-only column (0.5log₁₀) over 20 pore volumes. Additionally, two experiments with a column containing iron particles revealed 4log₁₀ and 5log₁₀ removal of rotavirus in the presence of 20 mg/L total organic carbon. Full-scale BSFs with iron particles removed >4log₁₀ MS2 for the duration of the experiment (287 days), while BSF with steel wool removed >4log₁₀ MS2 for the first 160 days. Plug flow for the BSF was shown to depend on uniformity between the iron oxide material and sand media grains. The results suggest that the duration of effective virus removal by iron-amended biosand filtration depends on source water conditions and the quantity and composition of iron material added. Overall, this study provides evidence that iron-amended BSFs may advance the field of point-of-use technologies and bring relief to millions of people suffering from waterborne diseases.     

Stress management in the health care setting: Matching interventions with patient coping styles.

Forty-six patients about to undergo preprosthetic oral surgery were presented with either a problem-focused, emotion-focused, or mixed-focus stress management intervention. The mixed-focus intervention produced the best overall response to surgery; the emotion-focused intervention produced the lowest adjustment levels. The Krantz Health Opinion Survey Information scale was the best overall predictor of response to surgery in conjunction with treatments. Better adjustment and satisfaction and lower self-reported pain were obtained when high information-preference subjects were given a problem-focused intervention and when low information-preference subjects were given an emotion-focused intervention; responses were relatively poor when mismatches occurred. Differences in relative use of problem- to emotion-focused coping as measured by the Ways of Coping Checklist were unrelated to adjustment or satisfaction, but differences in problem-focused coping were the best predictors of pain response in conjunction with treatments. Preoperative anxiety level was inversely related to adjustment and satisfaction and was directly related to pain, with the affective-unpleasantness component of pain primarily accounting for this association. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effects of focus of attention on anxiety levels and social performance of individuals with social phobia.

Self-focused attention has been linked to social anxiety and poor social performance, but the causal direction of this relationship has not been established. For this study, focus of attention was manipulated during a speech task, conducted in pairs for 38 individuals with generalized social phobia. Results indicated that intensifying self-focused attention increased anticipated anxiety and anxious appearance, regardless of whether the individual was giving a speech or passively standing before the audience. The self-focus manipulation also increased self-reported anxiety during the task, but only for individuals assigned to a passive role. Contrary to expectation, self-focused attention did not affect any measure of social performance. These results indicate that self-focused attention may play a causal role in exacerbating social anxiety. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Cognitive profiles of difficult-to-remediate and readily remediated poor readers: Early intervention as a vehicle for distinguishing between cognitive and experiential deficits as basic causes of specific reading disability.

Reading impaired first graders were given daily tutoring as a "first cut" diagnostic to aid in distinguishing between reading difficulties caused by basic cognitive deficits and those caused by experiential deficits. Reading achievement in most of these children was found to be within or above the average range after one semester of remediation. Children who were difficult to remediate performed below both children who were readily remediated and normal readers on kindergarten and first-grade tests evaluating phonological skills, but not on tests evaluating visual, semantic and syntactic skills. The results are consistent with convergent findings from previous research suggesting that reading problems in some poor readers may be caused primarily by phonological deficits. (PsycINFO Database Record (c) 2010 APA, all rights reserved)