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91.
We examined the molecular mechanism of metronidazole resistance by constructing a lambda-Zap II phagemid expression library with genomic DNA from a metronidazole-resistance strain of Helicobacter pylori. Twenty-two clones were found to have elevated MTZ resistances in XLOLR strain of E. coli. Phagemids belonging to the twenty two clones were extracted and then retransformed into the XLOLR strain of E. coli. After MTZ selection, five clones could confer metronidazole resistance consistently. According to Southern hybridization and DNA sequencing, the five clones contained a same locus, recA. In addition, transforming the five clones into BL21 strain of E. coli produced a higher resistance to MTZ. Interestingly, electroporation of one of the five phagemid clones into two MTZ sensitive H. pylori yielded MTZ resistant strains. Comparing amino acid sequence in MTZ resistant with sensitive isolates revealed two point mutations at this locus. Above results suggest that mutation in recA may be associated with metronidazole resistance of H. pylori. 相似文献
92.
Tony Maillet Jacques Barbier Jr. Daniel Duprez 《Applied catalysis. B, Environmental》1996,9(1-4):251-266
A 1% Pd catalyst (38% dispersion) was prepared by impregnating a γ-alumina with palladium acetylacetonate dissolved in acetone. The behaviour of this catalyst in oxidation and steam reforming (SR) of propane was investigated. Temperature-programmed reactions of C3H8 with O2 or with O2 + H2O were carried out with different stoichiometric ratios S(S =[O2]/5[C3H8]). The conversion profiles of C3H8 for the reaction carried out in substoichiometry of O2 (S < 1) showed two discrete domains of conversion: oxidation at temperatures below 350°C and SR at temperatures above 350°C. The presence of steam in the inlet gases is not necessary for SR to occur: there is sufficient water produced in the oxidation to form H2 and carbon oxides by this reaction. Contrary to what was observed with Pt, an apparent deactivation between 310 and 385°C could be observed with Pd in oxidation. This is due to a reduction of PdOx into Pd0, which is much less active than the oxide in propane oxidation. Steam added to the reactants inhibits oxidation while it prevents the reduction of PdOx into Pd0. Compared to Pt and to Rh, Pd has a higher thermal resistance: no deactivation occurred after treatment up to 700°C and limited deactivation after treatment up to 900°C, provided that the catalyst is maintained in an oxygen-rich atmosphere during the cooling. 相似文献
93.
J. M. Ballingall P. Ho J. Mazurowski L. Lester K. C. Hwang J. Sutliff S. Gupta J. Whitaker 《Journal of Electronic Materials》1993,22(12):1471-1475
InxGa1−xAs (x=0.25–0.35) grown at low temperature on GaAs by molecular beam epitaxy is characterized by Hall effect, transmission
electron microscopy, and ultrafast optical testing. As with low temperature (LT) GaAs, the resistivity is generally higher
after a brief anneal at 600°C. High-resolution transmission electron microscopy shows all the as-grown epilayers to be heavily
dislocated due to the large lattice mismatch (2–3%). When the layers are annealed, in addition to the dislocations, precipitates
are also generally observed. As with LT-GaAs, the lifetime shortens as growth temperature is reduced through the range 300–120°C;
also, the lifetime in LT-InxGa1−xAs is generally shorter in as-grown samples relative to annealed samples. Metal-semiconductor-metal photodetectors fabricated
on the material exhibit response times of 1–2 picoseconds, comparable to results reported on GaAs grown at low temperature,
and the fastest ever reported in the wavelength range of 1.0–1.3 μm. 相似文献
94.
J. H. Edgar C. A. Carosella C. R. Eddy Jr D. T. Smith 《Journal of Materials Science: Materials in Electronics》1996,7(4):247-253
The effects of nitrogen-beam voltage on the structure, stress, energy band gap and hardness of AIN thin films deposited on Si (111), Si (100) and sapphire (0001) by ion beam assisted deposition (IBAD) are reported. As the nitrogen-beam voltage was increased from 50 to 200 V, the stress and disorder in the AIN films increased as determined by X-ray diffraction, FTIR and Raman spectroscopy. The preferred orientation of the film's c-axis changed from completely normal to the film at 100 V, to a mixture of normal and in the plane of the film at 200 V. For AIN films deposited under the same conditions, the films were more highly oriented on sapphire (0001) than in Si (111). The hardness of the films increased from 18.2 to 23.7 GPa with the nitrogen-beam voltage, and possible reasons for this change in hardness are considered. 相似文献
95.
96.
Chemometrics has enjoyed tremendous success in the areas related to calibration of spectrometers and spectroscopy-based measurements. These chemometric-based spectrometers have been widely applied for process monitoring and quality assurance. However, chemometrics has the potential to revolutionize the very intellectual roots of problem solving. Are there barriers to a more rapid proliferation of chemometric-based thinking, particularly in industry? What are the potential effects of chemometrics technology and the New Network Economy (NNE) working in concert? Who will be the winners in the race for faster, better, cheaper systems and products? These questions are reviewed in terms of the principles of the NNE and in the promise of chemometrics for industry. What then is the state of multivariate thinking in industry? Several powerful principles are derived from an evaluation of the NNE and chemometrics which could allow chemometrics to proliferate much more rapidly as a key general problem-solving tool. 相似文献
97.
Cluster analysis in industrial market segmentation through artificial neural network 总被引:6,自引:0,他引:6
Market segmentation has commonly applied cluster analysis. This study intends to make the comparison of conventional two-stage method with proposed two-stage method through the simulated data. The proposed two-stage method is the combination of self-organizing feature maps and K-means method. The simulation results show that the proposed scheme is better than the conventional two-stage method based on the rate of misclassification. 相似文献
98.
SL Romney GY Ho PR Palan J Basu AS Kadish S Klein M Mikhail RJ Hagan CJ Chang RD Burk 《Canadian Metallurgical Quarterly》1997,65(3):483-492
OBJECTIVES: The age-dependence of the development of ventricular arrhythmias was studied in German shepherd dogs with inherited ventricular arrhythmias and sudden death. BACKGROUND: A colony of German shepherd dogs has been established that exhibit inherited ventricular arrhythmias and sudden death. The incidence of arrhythmias increases with age. Because ventricular tachycardia is associated with bradycardia, it was hypothesized that the increased incidence of arrhythmias was related to age-dependent slowing of heart rate. METHODS: Arrhythmia counts and RR intervals were measured from serial ambulatory ECG recordings obtained in 71 dogs (1-48 weeks). In addition, 19 dogs were challenged with phenylephrine (10 micrograms/kg i.v.) at 15, 28, and 45 weeks of age, 10 dogs were challenged with epinephrine (1 microgram/kg i.v.) at 3, 5, 7, 9, 11, 18, and 28 weeks of age, and 10 dogs were challenged at 28 weeks with epinephrine (2.5 micrograms/kg i.v.), before and after propranolol (0.5 mg/kg i.v.). RESULTS: The incidence and severity of ventricular arrhythmias increased between 7 and 28 weeks of age and decreased between 28 and 44 weeks of age. The age-dependent increase in the incidence of ventricular tachycardia was associated with age-dependent reductions in sinus rate. Baroreflex-mediated slowing of the heart rate unmasked arrhythmias in young animals that did not spontaneously display arrthythmias and exacerbated existing arrhythmias in older animals. However, the magnitude of baroreflex-induced bradycardia was similar from 7-18 weeks of age, yet the incidence of arrhythmias increased progressively. Moreover, the waning of ventricular arrhythmias in older animals was not associated with more rapid sinus rates. CONCLUSION: The risk for sudden death in dogs with inherited ventricular arrhythmias increases with age in part because of age-dependent slowing of heart rate and in part because of other heart-rate-independent factors. The correspondence between the development of ventricular tachycardia and sinus pauses is consistent with the hypothesis that ventricular arrhythmias are initiated by early afterdepolarization-induced triggered activity. 相似文献
99.
Buffer analysis has traditionally relied heavily on the use of traffic models with short range (exponentially decaying) autocorrelation, e.g. Poisson and Markov modulated Poisson processes. Recent literature has suggested the presence of traffic with slowly (hyperbolically) decaying autocorrelation functions; these sources collectively and individually seem to be exhibiting the phenomenon of long range dependence (LRD). Furthermore, it has been claimed that this sort of traffic may exhibit behaviour, when buffered, that is advantageous in that a smaller buffer may be required than would be the case with non-LRD traffic. This phenomenon has been named the crossover effect, and the authors quantify its relevance to ATM networking, using simulation studies of a homogenous mix of chaotic sources. These show that there is a crossover effect, but the key finding is that this effect is much nearer to a buffer length of zero than was predicted by previous theoretical studies, actually occurring at a value so low that it has no practical significance 相似文献
100.