Observations of bound Tween80 surfactant molecules on single-walled carbon nanotubes in an aqueous solution

The slow diffusion of Tween80 surfactant molecules in a single-walled carbon nanotube (SWCNT) aqueous dispersion was directly observed using the pulsed field gradient nuclear magnetic resonance method. The slow diffusion of Tween80 molecules was attributed to the strongly adsorbed molecules on the SWCNTs in the aqueous dispersion. The amount of bound Tween80 molecules was estimated to be approximately 12% of the total amount of Tween80 molecules, contributing to the stability of the SWCNT aqueous dispersion. Using dynamic light scattering and total organic carbon measurements, this SWCNT/Tween80 aqueous dispersion was found to be very stable for at least 3 weeks. The observed zeta potentials of this SWCNT dispersion are between −10 and 0 mV, indicating that the stability of the SWCNTs in the Tween80 solution was maintained by steric interactions between the small amount of adsorbed Tween80 molecules on the SWCNTs, while the effect of electrostatic interactions between adsorbed Tween80 was minimal. This proposed method to investigate the amount of bound Tween80 molecules on SWCNTs has potential benefits in the field of the production of functional materials and nano-toxicity assessments.     

On the efficiency of cautious schedulers for database concurrency control—Why insist on two-phase locking?

Thecautious scheduler, recently proposed for the concurrency control of database systems, never resorts to abortions or rollbacks for the purpose of concurrency control. The comprehensive performance evaluation study among different cautious schedulers and conventional non-cautious schedulers, however, has not yet been attempted. In this paper, we consider five scheduling algorithms and investigate their performance by means of simulation studies. Two of these algorithms are non-cautious; that is, thetwo-phase locking algorithm (2PL) (the most popular transaction scheduling algorithm in practical systems) and theconflict serializable algorithm (CSR) (a typical scheduling algorithm among those not using a locking mechanism; also calledD-serializable algorithm, conflict preserving serializable algorithm, orWW-serializable algorithm). The others are cautious scheduling algorithms modified from the above2PL andCSR; that is,cautious two-phase locking algorithm (C2PL), exclusive preclaimed two-phase locking algorithm (EP2PL), andcautious conflict serializable algoritm (CCSR). The results demonstrate the superiority of the cautious conflict serializable algorithm over the conventional two-phase locking algorithm, especially in the on-line system environment.This work was supported in part by the Ministry of Education, Science and Culture of Japan under Scientific Research Grant-in-Aid and in part by the Advancd Systems Foundations of British Columbia, Canada.     

Grasp and Delivery for Moving Objects on Broken Lines

This paper studies the following variant of the Vehicle Routing Problem that we call the Grasp and Delivery for Moving Objects (GDMO) problem, motivated by robot navigation: The input to the problem consists of n products, each of which moves on a predefined path with a fixed constant speed, and a robot arm of capacity one. In each round, the robot arm grasps one product and then delivers it to the depot. The goal of the problem is to find a collection of tours such that the robot arm grasps and delivers as many products as possible. In this paper we prove the following results: (i) If the products move on broken lines with at least one bend, then the GDMO is MAXSNP-hard, and (ii) it can be approximated with ratio 2. However, (iii) if we impose the “straight line without bend” restriction on the motion of every product, then the GDMO becomes tractable. Y. Asahiro’s research was supported in part by Grant-in-Aid for Young Scientists 15700021, and Research on Priority Areas 16092223. E. Miyano’s research was supported in part by Grant-in-Aid for Research on Priority Areas 16092223. Presently, S. Shimoirisa is with the Software Development Engineering Department, Products Development Center, Retail Information Systems Company, Toshiba TEC Corporation.     

Knowledge-directed inspection for complex multilayered patterns

Combining the knowledge-based processing with image processing is considered a key issue in the future of visual inspection of complex patterns such as multilayered semiconductor wafers. However, present technology restricts this combination, mainly because of the exhaustively long time usually required for each type of processing. To cope with this situation, a unique knowledge-directed image processing method is proposed, in which every image processing step is controlled in real time by parametric knowledge driven by design patterns. The resulting structure of the image processor is a pipeline, in which each piece of knowledge is embodied as a combination of a hardware processing unit and control unit. In this paper the types of knowledge and their implementation are explained, and an inspection machine for logic IC wafers based on this pipelined knowledgedirected image processing is introduced.     

Experimental study of the flow in helical circular pipes: Torsion effect on the flow velocity and turbulence

An objective of the present paper is to experimentally clarify the torsion effect on the flow in helical circular pipes. We have made six helical circular pipes having different pitches and common non-dimensional curvature δ of about 0.1. The torsion parameter β0, which is defined by β0 = τ/（2δ）1/2 with non-dimensional torsion r, are taken to be 0.02, 0.45, 0.69, 1.01, 1.38 and 1.89 covering from small to very large pitch. The velocity distributions and the turbulence of the flow are measured using an X-type hot-wire anemometer in the range of the Reynolds number from 200 to 20000. The results obtained are summarized as follows： The mean secondary flow pattern in a cross section of the pipe changes from an ordinary twin-vortex type as is seen in a curved pipe without torsion （toroidal pipe） to a single vortex type after one of the twin-vortex gradually disappears as β0 increases. The circulation direction of the single vortex is the same as the direction of torsion of the pipe. The mean velocity distribution of the axial flow is similar to that of the toroidal pipe at small β0, but changes its shape as β0 increases, and attains the shape similar to that in a straight circular pipe when ,β0 = 1.89. It is also found that the critical Reynolds number, at which the flow shows a marginal behavior to turbulence, decreases as ,β0 increases for small ,β0, and then increases after taking a minimum at ,β0 ≈ 1.4 as ,β0 increases. The minimum of the critical Reynolds number experimentally obtained is about 400 at ,β0 ≈ 1.4.     

Niobium–aluminum oxynitride prepared by ammonolysis of oxide precursor obtained through the citrate route

Oxynitrides in the (Nb_1−xAl_x)(O,N) quaternary system were prepared by ammonolysis of oxide precursor obtained through the citrate route. The products at 1000 °C were a mixture of Nb(N,O) and NbN_0.95 at the niobium end (x = 0) and amorphous Al(O,N) at the aluminum end (x = 1). A new cubic compound (A) appeared mixed with Nb(N,O) in the compositional range 0.1 ≤ x ≤ 0.4. Its almost pure product was obtained at x = 0.5. The X-ray diffraction pattern was rock salt type (Nb_0.56Al_0.44)(O_0.38N_0.37□₀₂₅) in F_m−3m with a = 0.43481(1) nm. The product showed superconductivity with T_c = 15 K. Its crystallinity was much improved and its superconducting volume fraction increased to 32% after its thermal annealing at 1100 °C in evacuated sealed tube. A second cubic compound (B), rock salt type Nb[(O,N)_0.85□_0.15] with a = 0.434 nm, was observed mixed with amorphous Al(O,N) in the as-prepared products of the range 0.6 ≤ x ≤ 0.9.     

Growth and coalescence of two collinear indentation cracks in glass

Subcritical growth and coalescence of two collinear cracks of different lengths were investigated using small Knoop indentation cracks in glass. Indentation cracks subjected to bending in water showed anomalous crack growth in terms of the stress intensity factor, K_I. The crack growth velocity, dc/dt, was initially high, decreased and thereafter increased with increasing K_I. The effective stress intensity factor, K_I,eff, was calculated by adding a term describing the state of residual stress to explain this anomalous growth. Before crack coalescence, a large crack showed a crack velocity higher than expected from the coalescent crack. The coalescent crack velocity increased with K_I,eff and the slope of dc/dt−K_I,eff curves differed from that for a single crack, depending on the crack length.     

Uptake and utilization of ectoine by halotolerant Brevibacterium sp. JCM 6894 subjected to osmotic downshock

The concentration changes of the cyclic amino acid ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyridine carboxylic acid) in Brevibacterium sp. JCM 6894 cells subjected to an osmotic downshock were investigated. When the cells grown in the presence of 2 M NaCl were suspended in deionized water, they immediately released about 60% of the ectoine synthesized intracellularly. During the subsequent incubation, we observed that both the extra- and intracellular concentrations of ectoine were reduced almost linearly with the incubation time. When ectoine was provided externally to the downshocked cells, a similar reduction in both intra- and extracellular ectoine concentrations was recognized. In addition, we observed an increase in ectoine accumulation at about 10 h of incubation, which indicates that ectoine was taken up by such downshocked cells in the absence of external Na+. Furthermore, the downshocked cells showed higher levels of survival, respiration, and growth in the presence of ectoine than in its absence. The ability to take ectoine up was induced in the cells grown in the presence of >0.25 M NaCl for >12 h. Thus, we conclude that even under the lower osmotic condition ectoine might be taken up and subsequently utilized by strain JCM 6894 subjected to the osmotic downshock, indicating that the uptake of ectoine by such cells occurred for the survival and growth of the cell itself rather than for cellular osmoregulation.     

Contribution of high p34cdc2 kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes

The present study was undertaken to clarify the relationship between the p34cdc2 kinase activity of in vitro-aged or enucleated rat oocytes and the premature chromosome condensation (PCC) of microinjected cumulus cell nuclei. Wistar rat oocytes were placed in vitro up to 120 min after the animal was killed. The p34cdc2 kinase activity of the oocytes decreased in a time-dependent manner. The incidence of PCC was higher when nuclear injection into intact oocytes was completed in 15-45 min rather than 46-120 min. When rat oocytes were enucleated for subsequent nuclear injection, the p34cdc2 kinase activity transiently increased soon after enucleation but drastically decreased after 30 min. Removal of the cytoplasm instead of the meta-phase-plate did not affect the p34cdc2 kinase activity even after 60 min. PCC occurred in intact and cytoplasm-removed oocytes but not in enucleated oocytes. In contrast, oocytes from BDF1 mice exhibited a p34cdc2 kinase level twice that of rat oocytes and supported PCC despite enucleation. The p34cdc2 kinase level of intact rat oocytes was reduced to the equivalent level of aged (120 min) or enucleated (+60 min) oocytes by a 45 min treatment with roscovitine, an inhibitor of p34cdc2 kinase. None of the roscovitine-treated oocytes supported PCC while half of the control oocytes did. When rat oocytes were treated with MG132, a proteasome inhibitor, delayed inactivation of the p34cdc2 kinase was observed in the MG132-treated oocytes. A significantly higher proportion of the MG132-treated oocytes supported PCC when compared with the control oocytes. Moreover, a higher proportion of MG132-treated and enucleated oocytes carried two pseudo-pronuclei after cumulus cell injection and developed to the two-cell stage when compared with the enucleated oocytes at the telophase-II stage. These results suggest that the decreased level of p34cdc2 kinase activity in aged or enucleated rat oocytes is responsible for their inability to support PCC of microinjected donor cell nuclei and that inhibition of p34cdc2 kinase inactivation by chemicals such as MG132 is in part effective for rat oocytes to promote PCC and further development.