Experience with radio isotope myelography from neurosurgical aspects (author's transl)

Experience with Radio Isotope Myelography is reported here. 169Yb-DTPA as a tracer was intrathecally injected at lumbar region in twenty three patients with various spinal cord lesions. The first scanning is perfomed after comfirming by gamma-camera that the tracer reaches to the lesion, the second and the third scannings are done according to the ascending rate of the tracer. (I) Normal scintimyelogram (A) In normal case, the shape of the Radio Isotope Myelogram well corresponds the shape of anatomical subarachnoid space. (B) In normal adult cases, the tracer comes up to the cisterna magna in 20-25 minutes after the lumbar injection. Therefore, the scintimyelographic diagnosis should be made not only by the shape but also by the ascending rapidity of the tracer. (II) Abnormal scintimyelogram Abnormal scintimyelograms could be summarized as following three categories. (A) "Delay": It means delay of the ascending of the tracer. Besides, "Transient delay" found in a case of Arnord-Chiari's malformation was proposed. (B) "Partial block": It meas a defect at the level of the lesion. This "Partial block" were observed in cases of spinal cord angioma, cervical spondylosis and spinal cord tumor etc. (C) "Complete block": It means the stagnation of the tracer below the lesion. To sum up, Rario Isotope Myelography, especially in partial block, can more easily and more sensitively represent the maximum extent of the spinal cord lesion than other myelographic study or angiographic one. On the other hand, we can not qualitatively diagnose about the lesions by its indistinct border.     

Effect of O2 on NO removal by ammonia radical injection using one-cycle sinusoidal power source

NO reduction experiment was performed by injecting ammonia radicals, which were externally generated by flowing the NH₃ gas diluted with Ar gas through a dielectric barrier discharge with a one-cycle sinusoidal-wave power source. The discharge was intermittently formed between coaxial cylindrical electrodes with a space of 1.5 mm at an applied peak-to-peak voltage of 3-25 kV. The generated radicals were injected into simulation gas (NO/O₂/N₂). The simulation gas contained 0-5.6% O₂, and the effect of O₂ on NO_x removal was discussed. The minimum reaction temperature for NO reduction was low when simulation gas contained O₂. High O₂ concentration (O₂=5.6%) in simulation gas, high repetition rate to NH₃, and high applied power to NH₃ decreased NO removal efficiency.     

Glasslike Transitions in Thin Polymer-Melt Films Due to Thickness Constraint

The shear properties of thin films of star and linear polyisoprene (PIP) melts under high pressure were investigated as a function of sliding velocity (shear rate) using the surface forces apparatus. The results were contrasted with their bulk rheological properties; effects of thickness constraint on the shear behavior were discussed. The melts of PIP in bulk exhibit Newtonian-like constant viscosity at least at low shear rates (frequencies), which suggests that individual molecules flow with lateral sliding motion. However, thin films of PIP melts show tribological features involving apparent shear-thinning behavior, indicative of the correlated motions in confined geometries. The shear-property change from bulk rheological behavior to thin-film tribological behavior along with the thickness decrease reflects the physical states and their transitions in the systems; the thickness constraint induces glasslike transitions. Effects of molecular weights and molecular architecture (star-branched or linear) on the shear properties are also discussed.     

A Technique to Eliminate Redundant Inter-Processor Communication on Parallelizing Compiler TINPAR

Optimizing inter-processor (PE) communication is crucial for parallelizing compilers for message-passing parallel machines to achieve high performance. In this paper, we propose a technique to eliminate redundant inter-PE messages. This technique utilizes data-flow analysis to find a definition point that corresponds to a use point where the definition and the use occur in different PEs. If several read accesses occurred in the same PE use the data defined at the same definition point in another PE, redundant inter-PE messages are eliminated as follows: only one inter-PE communication is performed for the earliest read access and the previously received data are used for the following read. In order to guarantee the consistency of the data, a valid flag and a sent flag are provided for each chunk of received data. The control of these flags is equivalent to the coherence control by the self invalidation on a compiler aided cache coherence scheme.     

Molecular beam epitaxial growth of SrO and CaO with RHEED intensity oscillation

SrO and CaO, related compounds of BSCCO superconductor, are grown onto SrTiO₃(100) substrates with molecular beam epitaxy(MBE) technique. During growth, the specular spots of RHEED patterns show intensity oscillations, indicating two dimensional growth. The periods of oscillations are utilized to calculate the atom fluxes. It is shown that sublimation processes of both Sr and Ca metals in the effusion cells are stable under the growth condition of oxide films. The periods of oscillations during the SrO growth are independent of substrate temperature, confirming that Sr atoms are oxidized immediately and stay on the substrate surface without re-evaporation. These informations are practically available for precise control of the atomic layer controlled MBE of BSCCO superconductor thin films.On leave from Superconductiivity Research Laboratory, ISTEC.     

Focal calcium monitoring with targeted nanosensors at the cytosolic side of endoplasmic reticulum

Ca²⁺ distribution is spatially and temporally non-uniform inside cells due to cellular compartmentalization. However, Ca²⁺ sensing with small organic dyes, such as fura-2 and fluo-4, has been practically applied at a single cell level where the averaged signal from freely diffusing dye molecules is acquired. In this study, we aimed to target azide-functionalized fura-2 (N₃-fura-2) to a specific site of subcellular compartments to realize focal Ca²⁺ sensing. Using scAVD (single-chain avidin)–biotin interaction and a copper-free click reaction system, we linked N₃-fura-2 to specifically-targeted scAVD protein fused with a red fluorescent protein mCherry, so that Ca²⁺ sensors conjugated with four N₃-fura-2 dyes with dibenzocyclooctyne (DBCO)-PEG4-biotin as a linker were generated at subcellular compartments in living cells. In cytoplasm, N₃-fura-2 showed a prolonged retention period after binding to scAVD. Furthermore, the reacted N₃-fura-2 was retained inside cells even after free dyes were washed out by methanol fixation. When scAVD was overexpressed on endoplasmic reticulum (ER) membranes, N₃-fura-2 was accumulated on ER membranes. Upon histamine stimulation, which increases cytosolic Ca²⁺ concentration, ER-localized N₃-fura-2 successfully sensed the Ca²⁺ level changes at the cytosolic side of ER membrane. Our study demonstrated specific targeting of N₃-fura-2 to subcellular compartments and the ability of sensing focal Ca²⁺ level changes with the specifically targeted Ca²⁺ sensors.     

Heavy metal precipitation by polycation-polyanion complex of PEI and its phosphonomethylated derivative

The removal of various heavy metal ions such as Cu2+, Co2+, Zn2+, Ni2+ and Pb2+ from aqueous solutions by precipitation with polyelectrolyte complex of PPEI and PEI was conducted. Heavy metal binding with PPEI was initially allowed to occur and then upon equilibration, PEI was added to initiate precipitation of the polyelectrolyte complex together with the heavy metal ion. The PPEI-PEI system was found effective for heavy metal scavenging purposes even in the presence of high concentrations of non-transition metal ions like Na+. Heavy metal concentration may be reduced beyond emission standards for industrial wastewaters. The PPEI-PEI polyelectrolyte complex was found to be more effective than traditional precipitation methods for the treatment of a representative electroless Ni plating waste solution.     

Development of correlation-based clustering method and its application to software sensing

The individuality of production devices should be taken into account when soft-sensors are designed for parallelized devices. Since it is expressed as differences of the correlation among measured variables, it is useful to cluster samples on the basis of the correlation among variables for adopting a multi-model approach. In addition, changes in process characteristics can be coped with in the same way. In the present work, a new clustering method, referred to as NC-spectral clustering, is proposed by integrating the nearest correlation (NC) method and spectral clustering. Spectral clustering is a graph partitioning method that can be used for sample classification when an affinity matrix of a weighted graph is given. The NC method can detect samples that are similar to the query from the viewpoint of the correlation without a teacher signal. In the proposed method, the NC method is used for constructing the weighted graph that expresses the correlation-based similarities between samples and the constructed graph is partitioned by using spectral clustering. In addition, a new soft-sensor design method is proposed on the basis of the proposed NC-spectral clustering. The usefulness of the proposed methods is demonstrated through a numerical example and a case study of parallelized batch processes. The performance of the proposed correlation-based method is better than that of the conventional distance-based methods.     

Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis

Non-alcoholic steatohepatitis (NASH) has pathological characteristics similar to those of alcoholic hepatitis, despite the absence of a drinking history. The greatest threat associated with NASH is its progression to cirrhosis and hepatocellular carcinoma. The pathophysiology of NASH is not fully understood to date. In this study, we investigated the pathophysiology of NASH from the perspective of glycolysis and the Warburg effect, with a particular focus on microRNA regulation in liver-specific macrophages, also known as Kupffer cells. We established NASH rat and mouse models and evaluated various parameters including the liver-to-body weight ratio, blood indexes, and histopathology. A quantitative phosphoproteomic analysis of the NASH rat model livers revealed the activation of glycolysis. Western blotting and immunohistochemistry results indicated that the expression of pyruvate kinase muscle 2 (PKM2), a rate-limiting enzyme of glycolysis, was upregulated in the liver tissues of both NASH models. Moreover, increases in PKM2 and p-PKM2 were observed in the early phase of NASH. These observations were partially induced by the downregulation of microRNA122-5p (miR-122-5p) and occurred particularly in the Kupffer cells. Our results suggest that the activation of glycolysis in Kupffer cells during NASH was partially induced by the upregulation of PKM2 via miR-122-5p suppression.