Nested polymerase chain reaction (PCR) and multiple cloned antibody capture PCR for the examination of serum hepatitis B virus DNA in negative hepatitis B surface antigen patients suffering from liver diseases

In order to find out rapidly the causes of the liver diseases suffered by patients with negative hepatitis B surface antigen (HBsAg), nested polymerase chain reaction (PCR) and multiple cloned antibody capture PCR techniques were established to examine serum hepatitis B virus (HBV) DNA. By using both techniques along with the examination of hepatitis C virus (HCV) infection, the causes of chronic liver diseases with negative HBsAg were studied. It is found that nested-PCR can increase the sensitivity of single PCR more than 1,000 fold and multiple cloned antibody capture-PCR can detect concentration of HBV DNA as low as 0.1-0.01 pg/L. HBV DNA positive patients were found in 45.5%, 30.8%, 13.3% and 100% respectively of the patients suffering from liver cirhosis with negative HBsAg (group A, 22 cases), chronic hepatitis with negative HBsAg (group B, 13 cases), normal subjects with negative HBsAg and positive hepatitis B core antibody (HBcAb, group C, 30 cases) and liver cirhosis with positive HBsAg and negative HBeAg (group D, 12 cases). HBV DNA can be also found in the serum of HBsAb positive patients and subjects supposed to be healthy, 81.8% and 53.8% of the patients were infected with HBV and/or HCV in group A and group B respectively. All these results suggest that nested-PCR and multiple cloned antibody capture-PCR are rapid and highly sensitive methods for detection of serum HBV DNA. HBV infection is an important cause of chronic liver diseases in patients with negative HBsAg. The causes of most of the HBsAg-negative chronic liver diseases are related with infection of viruses. The clinical significance of serum HBsAb in naturally infected patients should be reconsidered.     

利用射线路径反演层状介质速度的方法

本文根据欧拉方程对于连续层状介质进行了方程推导，得出一个波传播的射线路径公式，利用中 公式导出了ＶＳＰ资料处理中的速度反演公式。     

一种按时间切换的最速控制方法

基于初始状态空间下的子空间概念，提出了一种按时间进行切换的最速控制方法，不需要求取开关线或开关面。数字仿真结果表明，采用这一方法是完全可行的。     

基于功能的数据箱管理系统的设计

本文介绍数据箱库ＢＯＸＤＢ的模型及数据箱结点结构，并给出它的数据管理方法，方法是一组以Ｃ编写的专用库函数。     

Microphase structure and calculations of the interphase in poly (styrene-b-methylphenylsiloxane) diblock copolymers: a comparative study from small-angle X-ray scattering,electron spectroscopic imaging and solid-state nuclear magnetic resonance

The microphase structure of a series of poly (styrene-b-methylphenylsiloxane) (PS-b-PMPS)-diblock copolymers with various compositions and molecular weights has been studied by small-angle X-ray scattering, electron spectroscopic imaging and solid-state nuclear magnetic resonance spectroscopy. This diblock copolymer system is miscible within a distinct range of temperature and molecular weight exhibiting an upper critical solution temperature. The three chosen methods which complement each other can be applied to the chemically unmodified model system. The results of the various measurements correlate to describe the microphase structure, the domain size and the interphase.     

威远构造与震旦系油气勘探

本文扼要地论述了威远构造从30年代到60年代震旦系最古老气藏发现的漫长历史,以及直到90年代初震旦系天然气勘探重新获得突破的曲折过程。这一历史过程,也是四川盆地复杂碳酸盐岩裂缝性气藏勘探的剪影。     

中国选煤的现状与发展

论述了中国选煤是洁净煤的基础、选煤现状，分析了能源结构，指出了今后中国选煤技术开发的重点。     

对石化企业深化分配制度改革的思考

当前石化企业分配制度及相关管理体系中存在的问题，主要体现在竞争机制、岗位设置、绩效考核、薪酬制度和职务消费等五个方面。通过对存在问题的思考，提出了夯实基础、理顺关系、科学设计薪酬制度的深化分配制度改革的思路。     

超细分子筛材料的合成与应用研究进展

综述了近几年超细分子筛的合成与应用方面的研究进展,重点介绍了几种常用且研究较多的分子筛,主要包括Y,ZSM-5,β,Silicalite-1,ZSM-35,MCM-22等分子筛。对超细分子筛的合成方法及其在石化、环保和生物等领域的应用进行了介绍,并对分子筛复合材料的研究及应用的最新动态进行了评述,说明超细分子筛材料的应用领域仍在不断拓宽,超细分子筛复合材料的研究将会成为分子筛材料领域未来的重要发展方向。