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31.
Silica particles are mainly used for the concentration of nucleic acid for diagnostic purposes. This is usually done under acidic or chaotropic conditions that will demolish most of the living organisms and prevent the application of other diagnostic tests. Here we describe the development of a method for the capturing and concentration of Bacillus spores using silica magnetic particles to enable fast and sensitive detection. We have shown that capturing various Bacilli spores via silica magnetic particles is limited, with large differences between spore batches (42 +/- 25%). The hydrophobic exosporium layer of spore limits the capture by the hydrophilic silica beads. Partial removal of Bacillus exosporium increases capture efficiency. To increase capturing efficiency without harming the spores' viability, a cationic lipid, didecyldimethylammonium bromide (DDAB), was used as a coat for the negatively charged silica particles. DDAB treatment increased capture efficiency from 42% to more than 90%. Using this method, we were able to capture as few as 100 Bacillus anthracis spores/mL with 90% efficacy. Release of captured spores was achieved by the addition of albumin. The capture and release processes were verified by plating and by flow cytometry using light scatter analysis. The method is simple, efficient, easy to operate, and fast. 相似文献
32.
K. De Kerpel B. Ameel H. Huisseune C. T’Joen H. Canière M. De Paepe 《International Journal of Heat and Mass Transfer》2012,55(4):1179-1188
Adiabatic two-phase flow of refrigerant R-134a in a hairpin was studied. The hairpin consists of a smooth tube with an inner diameter of 8 mm and with a bend radius of 11 mm. Because of the forces exerted on the flow in the bend, the flow needs to redevelop downstream of the U-bend. The effects of this phenomenon on the pressure drop are studied and linked to visual observations of the flow. Pressure drop and videos of the flow behaviour are recorded in the straight sections upstream and downstream of the bend. These are then compared to the flow and pressure drop for developed flow. The pressure drop downstream of the bend was consistently higher than that for developed flow. It exceeded the pressure drop for developed flow by an average of 30% for all data points. Each video of the flow behaviour was reduced to a single image by calculating the standard deviation of the time signal of each pixel. The standard deviation profiles were compared in order to quantitatively evaluate the change of the flow behaviour. The flow recovery downstream of the bend stretches out over more than 30 tube diameters. 相似文献
33.
Leitner G Sela S Hammer-Muntz O Zivotofsky D Weisblit L Chaffer M Zamir S 《The Journal of dairy research》2009,76(1):1-5
Staphylococcus haemolyticus is a pathogen frequently isolated from dairy cows and small ruminants. However, it always appears in only a few animals and not as a major pathogen. Recently, in a dairy goat herd of approximately 250 milking animals, 25.6% (46/180 goats) had milk cultures with atypical highly mucoid colonies accompanied by elevated somatic cell counts. The isolates were identified as Staph. haemolyticus. The present study describes the steps used in an attempt to identify the bacterium and to compare it with other coagulase-negative staphylococci (CNS) including Staph. haemolyticus. Species identification performed with the API STAPH-IDENT 32 kit showed >99.4% identity confirmed by 16S rDNA sequencing tests. Microscopically the atypical Staph. haemolyticus strains showed unique cuboidal tetrad clusters reminiscent of those of the genus Sarcina. The outbreak caused by an atypical CNS underlines the need for accurate biochemical and genetic methods for ultimate identification of CNS to the species level. 相似文献
34.
Mark Shneider Hanna Dodiuk Reshef Tenne Shmuel Kenig 《Polymer Engineering and Science》2013,53(12):2624-2632
Closed‐cage (fullerene‐like) nanoparticles (NPs) of WS2 are currently produced in large amounts and were investigated as additives to thermoplastics and thermosetting polymers. The nanoinduced morphology and the resulting enhanced fracture toughness of epoxy/WS2 nanocomposites were studied. The morphology of the epoxy nanocomposites was induced by controlled WS2 surface chemistry. The WS2 NPs used were either untreated or chemically treated with acryloxy, which is compatible, and alkyl silane, which is incompatible, respectively, with the epoxy matrix. In the case where the acryloxy silane was used to treat the WS2 particles, good dispersion and compatibility were obtained in the epoxy resin. Moreover, a distinct nodular morphology was induced on fracture as a result of nucleation by the compatible NPs. In the case where the alkyl silane treatment was used cavitation morphology was induced, following mechanical loading, which is the result of incompatibility with the epoxy resin. The fracture toughness results showed an increase of 70% for nanocomposites contains alkyl‐treated WS2 compared with the neat epoxy. Modeling of the nodular morphology enabled the determination of optimal concentration of the WS2 in epoxy (0.3% by weight). Two main fracture mechanisms were observed, crack bowing around the nodular boundaries in the case of compatibility between the nanoparticle and the epoxy and particle‐induced cavitation in the case of incompatibility, respectively. These results are of significant importance both for epoxy‐based adhesives and fiber composites. POLYM. ENG. SCI., 53:2624–2632, 2013. © 2013 Society of Plastics Engineers 相似文献
35.
A chemical kit for field determination of traces of explosives on hands of suspects is described. The system involves three components: dry sampling devices (‘‘detection buttons''), and two tubes of chemical reagents. It detects virtually all the common military explosives, and there is also an option to detect home-made explosives based on inorganic fertilizers. Detection is based on two well known chemical reactions for which optimal conditions have been developed. In stage I polynitro aromatics are detected by the formation of colored Meisenheimer complexes. In stage II organic nitrates and nitramines are consequently detected by modified Griess test. 相似文献
36.
Emanuel Gluskin Shmuel Miller 《International Journal of Circuit Theory and Applications》2013,41(9):988-997
The determination of the time averages of continuous functions or discrete time sequences is important for various problems in physics and engineering, and the generalized final‐value theorems of the Laplace and z‐transforms, relevant to functions and sequences not having a limit at infinity, but having a well‐defined average, can be very helpful in this determination. In the present contribution, we complete the proofs of these theorems and extend them to more general time functions and sequences. Besides formal proofs, some simple examples and heuristic and pedagogical comments on the physical nature of the limiting processes defining the averaging are given. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
37.
Inorganic oxidizing agents are used in various explosive mixtures that are analysed by analytical chemists. Among them are dynamites, ANFO, black powder, match heads, pyrotechnical devices and home-made bombs. The crucial step in post explosion analysis is the extraction and purification of the explosive residues from the debris. A unique, fast and selective extraction technique for inorganic oxidizing agents is described in this paper. The debris are first extracted with water to yield an aqueous solution of the ions. Oxidizing anions such as nitrate and chlorate, are selectively transfered into the organic phase by the use of lipophilic quarternary ammonium cations. Other anions such as carbonate, sulphate and nitrite are not extracted into the organic phase under these conditions. The extracted anions are then identified by infra-red spectrometry and chemical spot tests. 相似文献
38.
We define the convexity rank of a set of points to be the portion of mutually visible pairs of points out of the total number of pairs. Based on this definition of weak convexity, we introduce a spectral method that decomposes a given shape into weakly convex regions. The decomposition is applied without explicitly measuring the convexity rank. The method merely amounts to a spectral clustering of a matrix representing the all‐pairs line of sight. Our method can be directly applied on an oriented point cloud and does not require any topological information, nor explicit concavity or convexity measures. We demonstrate the efficiency of our algorithm on a large number of examples and compare them qualitatively with competitive approaches. 相似文献
39.
Implementation of a new approach to the nonlinear noisy image restoration problem, which is feasible for large images, is described. The approach is based on a vector space representation of images and on using the conjugate gradient algorithm to solve a least squares minimization problem. Computer simulations yield good results with relatively little computational cost. 相似文献
40.
The laminar boundary layer equations for the compressible flow due to the finite difference in rotation and temperature rates are solved for the case of uniform suction through the disk. The effects of viscous dissipation on the incompressible flow are taken into account for any rotation rate, whereas for a compressible fluid they are considered only for a disk rotating in a stationary fluid. For the general case, the governing equations are solved numerically using a standard finite element scheme. Series solutions are developed for those cases where the suction effect is dominant. Based on the above analytical and numerical solutions, a new asymptotic finite element scheme is presented. By using this scheme one can significantly improve the pointwise accuracy of the standard finite element scheme. 相似文献