Suppression of cell growth by ectopic expression of N-cadherin

We found that ectopic expression of N-cadherin in 3Y1 caused tight association of cells and, thereby, substantially suppressed cell growth. N-cadherin expression inhibited neither tyrosine phosphorylation of cellular proteins, GTP uptake onto Ras, nor activation of MAP kinase, suggesting that it does not directly interfere the Ras-MAP kinase pathway. However, co-expression of N-cadherin with dominant negative Ras, S17N Ras, showed synergestic growth inhibitory effect, suggesting that N-cadherin signaling antagonizes the Ras-MAP kinase signaling. In addition, we found that N-cadherin yielded cell-cycle arrest at G0/G1 phase. These results strongly suggest that N-cadherin cell adhesion machinery works as a negative controller of cell cycle in 3Y1 and this growth suppressive function of cadherin is distinct from the epithelial morphogenetic function.     

Variation of Fructooligosaccharides and their Metabolizing Enzymes in Onion Bulb (Allium cepa L. cv. Tenshin) During Long-term Storage

ABSTRACT: The objective of this study was to assess the status of fructooligosaccharides (FOS) in onion bulbs ( Allium cepa L. cv. Tenshin) and their metabolizing-enzymes—1-fructoexohydrolase (1-FEH), 1-kestose hydrolyzing enzyme (1-KH), fructan:fructan 1^F-fructosyltransferase (1-FFT) and fructan:fructan 6^G-fructosyltransferase (6G-FFT)—during storage at 15°C. Fructose varies slightly, whereas 1-kestose peaked after 6 wk and then decreased progressively during the last 18 wk of storage. Lower degree of polymerization (DP) 3 to 6) FOS, higher (DP 7 to 12) FOS, total FOS, and total carbohydrates showed similar and close patterns during 24 wk. They varied slightly at the beginning of the storage period; afterward they decreased progressively and regularly during the last 20 wk of storage. 1-FEH and 1-KH activities were low but peaked abruptly after 12 and 16 wk, respectively, after which they decreased to levels higher (1-FEH) or similar (1-KH) to those observed at the beginning of the storage. Surprisingly, 1-FFT activity showed similar pattern to the variation of 1-KH hydrolyzing activity; on the other hand, 6G-FFT, although higher, was stable during 16 wk but decreased after that. The results allowed us to associate FOS to the dormancy and sprouting states, and the peaks of the degrading enzymes were shown to signal the release of dormancy of onion bulb.     

Development of a deep-hole, laser-guide boring tool: the boring of workpieces with a thin wall and an inclined prebored hole

A laser-guided BTA tool has been developed to prevent axial hole deviations. Four kinds of experiments were conducted to examine the performance of this tool in detail and to determine its practical application using a duralumin (A2017-T4) workpiece with a prebored 108-mm diameter hole. The first experiment was performed to examine the conditions of the hole deviation in the case of normal deep-hole boring. The second was to examine whether the tool corrects for the stated hole deviation. The third was to examine whether the tool can go straight through to the target without shifting toward a thin wall (t = 0.7 mm) on one side of the workpiece. The fourth was to examine whether the tool can be guided toward the target without affecting a prebored hole, which was inclined to radial direction at 100 μm for a hole length of 100 mm. The experiments were performed with a rotating tool-stationary workpiece system. Rotational speed was 270 rpm and feed ws 0.125 mm/rev. Tool diameter was 110 mm. The results show that the tool can be guided to go straight through toward the target despite the disturbances.     

Remarkable effect of addition of In and Pb on the reduction of N2O by CO over SiO2 supported Pd catalysts

The effect of addition of In and Pb on the reduction of N₂O by CO was studied over SiO₂ supported Pd catalysts, using a closed gas circulation system as well as in-situ infrared spectroscopy. Formation of intermetallic compounds such as Pd_0.48In_0.52, Pd₃Pb and Pd₃Pb₂ was observed which caused a drastic enhancement of the rate of N₂ formation. The infrared spectroscopic analyses revealed a weakening of the adsorption strength of CO on Pd metal by the formation of intermetallic compounds, which is likely the main reason for the enhancement of the reaction rate. From a kinetic investigation as well as in situ FT-IR observation during the N₂O-CO reaction, a redox mechanism was proposed involving the oxidation of the surface by N₂O followed by its reduction by CO. Over Pd/SiO₂, the former process seems to be the rate limiting step because of the inhibition of N₂O activation by strongly adsorbed CO. By adding In or Pb, the rate limiting step shifted to the latter process, which resulted in a large enhancement in the rate of N₂ formation.     

Depth profiling study on the migration of tritium in titanium induced by deuterium-ion bombardment

A thin titanium layer with uniformly absorbed tritium (T/Ti ˜1.0) was bombarded by 390 keV D₃⁺ ions (130 keV per deuteron). Bombardment was performed at low (111 K) and room temperatures up to fluences of 5.9 × 10¹⁸ D/cm² and 3.0 × 10¹⁸ D/cm², respectively. Depth profiles of tritium up to a depth of 0.8 mg/cm² (˜1.8 μm) were measured and the change of the profile with fluence was investigated by means of the T(d, )n nuclear reaction. At both of the temperatures, a dip was formed on the depth profile of tritium at the depth around the projected range, indicating that the deuteron bombardment induced the migration of tritium against the concentration gradient. At the low temperature, the dip showed a gradual growth with fluence and saturation of the growth at the higher fluences, which could not be described by the existing model for isotope mixing. The spectrum of protons from the D(d, p)T reaction obtained in the same measurement suggested that the release of deuterium suddenly started at the final stage of the present bombardment. The dip formed at room temperature was larger than that at the low temperature. The migration of tritium induced by the bombardment is discussed on the basis of the experimental results obtained.     

Polarisation conservation in single-mode fibres

There exist proper orthogonal elliptical polarisation states, which are maintained over long fibre length without converting. When one of the proper polarisation states is excited, orthogonal polarisation less than ?40 dB is confirmed after 5 km single-mode fibre transmission. It is also proposed that twisted single-mode fibres show a high degree of polarisation for any incident polarisation state. The polarisation characteristics are hardly affected by external perturbations.