Evaluation of environmentally friendly lubricants for cold forging

The authors proposed double-layer-type environmentally friendly lubricants, which were composed of an undercoat, superior in adhering to a material, and an overcoat, superior in reducing the friction between the material and the die. The performance of these lubricants for cold forging was evaluated by the ring compression test, the combined forward rod-backward can extrusion-type friction test and the combined forward conical can-backward straight can extrusion-type friction test. The double-layer-type lubricants showed comparable friction characteristics and anti-pick-up properties to a conversion coating lubricant, when the film thickness and surface treatment before coating were improved. In a practical application by cold multistage forging, the double-layer-type lubricants showed a similar performance to a conversion coating lubricant.     

Ionic liquid enables simple and rapid sample preparation of human culturing cells for scanning electron microscope analysis

Ionic liquid is a kind of salt that stays in a molten state even at room temperature. It does not vaporize at all in vacuum and facilitates electrical conductivity to the sample surfaces for observations with a scanning electron microscope (SEM). In this study, we used an ionic liquid in SEM for the first time to observe fixed human culture cells. The condition for the cell culture using wrapping sheets and SEM settings were varied to elucidate the optimized protocol. Compared to samples prepared by the conventional way, the ionic liquid‐treatment of samples gave SEM images of the cellular ultra structures in more detail, enabling observation of microvilli that made bridges between separated cells. In addition, the ionic liquid treatment is less time consuming as well as less laborious compared with the conventional way that includes dehydration, drying, and conductivity treatments. Totally, we concluded the ionic liquid is a useful reagent for SEM sample preparation. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.     

Suppression of inducible nitric oxide synthase expression and amelioration of lipopolysaccharide-induced liver injury by polyphenolic compounds in Eucalyptus globulus leaf extract

Eucalyptus leaf extract (ELE) is rich in hydrolyzable tannins. We examined the effects of ELE and its constituents on lipopolysaccharide (LPS)-induced liver injury in mice. Mice fed a diet supplemented with 1% ELE were intraperitoneally administered LPS. Six hours later, the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly lower in the ELE-supplemented mice than in the controls; LPS-induced hepatic inducible nitric oxide synthase (iNOS) expression was also suppressed. ELE lowered LPS-stimulated iNOS expression in cultured RAW 264.7 macrophages. Furthermore, the aglycones of hydrolyzable tannins, gallic acid (GA) and ellagic acid (EA), inhibited iNOS induction to a greater extent than did ELE (15-fold higher). When mice were fed a 1% GA or EA diet, the increase in the serum ALT and AST activities and hepatic iNOS expression in response to the LPS challenge were significantly attenuated. Thus, hydrolyzable tannins in ELE ameliorate LPS-induced liver injury.     

Evaluation of three-dimensional effects in short deep beams using a rigid-body-spring-model

Three-dimensional (3-D) effects in short deep beams without stirrups that failed in shear were investigated experimentally and analytically. Two deep beams with a shear span to depth ratio (a/d) of 0.5 and with different beam widths were tested. The effect of beam width on load-carrying capacity, failure mode, crack pattern and 3-D behavior was investigated, and shape effect due to beam width was clarified. In addition, the beams were analyzed by the 3-D rigid-body-spring model (RBSM). RBSM is a discrete form of modeling that presents realistic behavior from cracking to failure, and 3-D RBSM is applicable to simulate 3-D behavior as well as the confinement effect of concrete. Analytical results in terms of load–displacement curves and crack pattern are compared with the experimental results. Three-dimensional deformations, strut widths and cross-sectional stress distribution are investigated analytically and compared with the experimental results to determine 3-D behavior in detail. The 3-D effects in short deep beams are clarified.     

Nonintrusive appliance load monitoring based on integer programming

This paper presents a new nonintrusive appliance load monitoring technique based on integer programming. Nonintrusive appliance load monitoring is the problem of identifying the operating conditions of electric appliances in a house by observing only the overall load current and voltage. Since the overall load current is expressed as a superposition of the currents of the operating appliances, the monitoring problem can be formulated as an integer quadratic programming problem by expressing the operating conditions as integer variables. This problem is solvable with a sufficiently small computational burden thanks to the recent development of commercial software. The proposed method does not require relearning even when a new appliance is installed in the house. Furthermore, the proposed formulation is applicable to cases in which some appliance has multiple modes, and cases in which some appliances of the same type are operating simultaneously. The usefulness of the proposed technique is verified by experimental results. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 174(2): 18–25, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.21040