Efficient directional cloning of recombinant adenovirus vectors using DNA-protein complex

We describe an efficient cloning system utilizing adenoviral DNA-protein complexes which allows the directional cloning of genes into adenoviral expression vectors in a single step. DNA-protein complexes derived from a recombinant adenovirus (AVC2.null) were isolated by sequential use of CsCl step gradients followed by isopycnic centrifugation in a mixture of CsCl and guanidine HCl. AVC2.null is an adenoviral expression vector containing unique restriction sites between the human CMV-IE promoter and the SV40 intron/polyadenylation site. Transgenes were prepared for cloning into this vector by introduction of compatible restriction sites by PCR. A vector expressing rat granulocyte-macrophage colony-stimulating factor (GM-CSF) was constructed using DNA-protein complex as well as by traditional recombination techniques. The efficacy of our adenoviral cloning system utilizing DNA-protein complex was two logs higher than that seen using homologous recombination. All viruses generated by directional ligation of the insert into the vector DNA-protein complexes contained the desired transgene in the correct orientation. This technique greatly simplifies and accelerates the generation of recombinant adenoviral vectors.     

Fiberscope-assisted evaluation of prereconstructed and postreconstructed external auditory canal

The malignant tumors of the external auditory canal often invade the middle ear area through the narrow canal, so it is difficult to determine the surgical excisional margin. A patient undergoing external auditory canal reconstruction following excision of a squamous cell carcinoma using a chondrocutaneous flap and fiberscope-assisted preoperative and postoperative diagnoses are described. We obtained good patency of the canal postoperatively. This technique is quite simple and easy to use. We believe fiberscope-assisted surgery is useful for extracting lesions in small areas. In the future, its application will extend to the area of plastic surgery.     

松材线虫病害高光谱时序与敏感特征研究

采用高光谱仪ASD FieldSpec Pro FR,连续观测了健康和发病马尾松针叶的时序高光谱,分析了松材线虫病害光谱的时序特征、敏感时期和敏感特征。结果表明:与健康马尾松相比,病害马尾松时序光谱差异较大;病害首先造成红边区域内光谱反射率减低,然后再出现红边蓝移的2阶段光谱变化规律;感染松材线虫的马尾松9月初已经出现了病态植被典型的光谱特征;近红外平台内最大的一阶微分值、红边内一阶微分的总和(SDr)与蓝边内一阶微分的总和(SDb)的比值等是指示病害发生的显著性高光谱特征。     

Optimal Performance in Tracking Stochastic Signal Under Disturbance Rejection

The analysis method of optimal tracking performance is proposed for multiple‐input multiple‐output (MIMO) linear time‐invariant (LTI) systems under disturbance rejection. An H₂ criterion of the error signal between the output of the plant and the reference signal is used as a measure for the tracking performance. Spectral factorization is applied to obtain the optimal solution of the system tracking error. The explicit expressions are derived for this minimal tracking error with respect to random reference signals under disturbance rejection. It is shown that the nonminimum phase zeros, the zero direction, the unstable poles, the pole direction of a given plant, statistical characteristics of the reference input signal, and disturbance signal have a negative effect on a feedback system's ability to reduce the system error with disturbance rejection. The results show that the optimal tracking performance will further be damaged because of disturbance rejection. Some typical examples are given to illustrate the theoretical results.     

即时定向新闻采集技术研究

互联网的迅速发展带动了信息量的爆炸性增加。如何更快地采集所需信息一直是国内外研究和开发的热点。近年来,不断增长的对特定信息(例如特定领域的新闻)的需求要求有针对性地从指定的网站即时采集相关信息。这些新闻一般具有不可预见性、更新频率较快、时效性强等特点。这要求我们必须能针对这些特点实现即时定向的采集。本文提出了一种有效抓取网页并进行分析的方法,实践表明取得了满意的效果。     

Physical properties of transition to chaos in RF-driven Josephson junction analog

An analog circuit is used to investigate the behavior of the transition to chaos in the rf-driven Josephson junction under the variation of excitation amplitude and frequency with McCumber parameter >1. A series of transitions is observed, including a hysteretic jump, symmetry breaking, period doubling, and intermittency prior to chaos, and the transition boundaries are given in a state diagram in the vs. parameter space. Thus, the sequence of transitions to chaos can be inferred from this diagram. Based on these results, the harmonic balance method and Floquet theory are applied to study the instability of the transition series. It is suggested that all these transitions to chaos can be explained as parametric excitations. In addition, the transition boundaries in the state diagram can be satisfactorily computed from the conditions with a Floquet multiplier =±1.     

齿轮类零件电子束焊接结构的合理性分析

综述了齿轮类传动零部件采用电子束焊接加工的优越性，指出零件在不同结构尺寸、不同使用环境以及不同工艺状态下应选用与电子束焊接工艺相适应的焊缝结构，并对不同零件的焊缝接头结构形式的合理性进行了深入分析。     

Site occupations and ordering behaviour of O phase in Ti2AlNb—based intermetallics

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基于注意力机制和卷积神经网络的异步电动机三相电压不平衡损耗研究

配电网中三相电压不平衡对异步电动机损耗会造成较大影响。运用等效电路公式分析三相电压不平衡影响下电动机损耗存在精度不稳定、需要参数过多且数学模型过于复杂等问题。针对以上问题,提出一种基于注意力机制和卷积神经网络(CNN)的异步电动机损耗评估方法。该方法将实测电机数据作为输入,引入注意力机制为输入特征赋予不同权重;采用卷积层和全连接层组成的CNN构架对异步电动机实测数据进行学习,最后完成损耗评估。以现场试验得到的电机损耗数据作为实际算例,该方法评估损耗与实测损耗平均误差仅为0.717%和0.549%,并与其他典型机器学习算法进行对比,结果表明所提方法具有更好的损耗评估性能。     

电子束钎焊温度模糊控制系统

将被焊工件温度模糊控制技术应用于电子束扫描钎焊中,所研发的系统集成了电子束焊接过程控制、电子束扫描轨迹控制、工件温度模糊控制、焊接数据高速采集与存储等多项功能.通过扫描轨迹的编辑与控制,可以适应各种形状曲线钎焊缝的温度场要求;在电子束对工件进行扫描加热的同时,通过温度采集装置实时得到被焊工件的温度信号并与设定值进行对比从而得到温度偏差及偏差的变化率作为模糊控制器的两个输入变量,以模糊控制器输出控制量调节电子束流大小,从而实现钎焊温度的闭环控制.控制系统具有响应速度快、稳定时间短、稳态误差小、超调量小等特点,表明在电子束钎焊过程中采用模糊控制技术可以得到非常理想的控制效果.