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该文报道了一种基于酶级联扩增的电化学核酸适体检测腺苷的新方法。当腺苷存在时,滚环扩增的引物通过E.coli DNA连接酶的作用与通过巯基组装在金电极上的固定探针连接。在pHi29 DNA聚合酶的作用下,滚环扩增反应进行并产生一条与环形探针完全互补的长的单链,然后将大量金纳米粒子标记的核酸探针与滚环扩增的产物杂交。该传感界面电化学行为的结果表明,通过酶级联扩增的方法提高了检测腺苷的阻抗响应灵敏度,且选择性和重现性良好。用于腺苷的检测时,其线性范围为2.0μmol/L~100μmol/L,最低检测浓度为2.0μmol/L,表明该传感界面的设计可作为一种通用方法而有望用于其它目标物的检测。  相似文献   
23.
In this paper we present an extension to the work of Björck et al. for computing the determinants of matrices with univariate or bivariate polynomials as entries to multivariate case. The algorithm supports parallel computation and has been implemented on a multi-core cluster computer system. We show how to use our approach to calculate two unsolved problems, which arise from computational geometry optimization and electric power engineering, and analyze the time complexity as well as bits complexity.  相似文献   
24.
Share price trends can be recognized by using data clustering methods. However, the accuracy of these methods may be rather low. This paper presents a novel supervised classification scheme for the recognition and prediction of share price trends. We first produce a smooth time series using zero-phase filtering and singular spectrum analysis from the original share price data. We train pattern classifiers using the classification results of both original and filtered time series and then use these classifiers to predict the future share price trends. Experiment results obtained from both synthetic data and real share prices show that the proposed method is effective and outperforms the well-known K-means clustering algorithm.  相似文献   
25.
本文所阐述的基于Web的作业管理系统.利用网络编程.实现在一个平台上进行作业的上交、批改、以及成绩的统计和查询等操作.为教学的进行提供了有效便捷的手段。  相似文献   
26.
Perovskite light-emitting diodes (PeLEDs) show promising prospects in the wide color gamut display owing to their ultra-narrow full width at half maximum (FWHM). However, up to now, all perovskite white LEDs integrated by standard red, green, and blue perovskite emitters, namely, monolithic white PeLEDs (WPeLEDs), have been rarely reported, owing to facing some issues, e.g., solvent incompatibility in solution technique, ion exchange, and energy transfer between different emission centers. Herein, centered on these issues, an optimal intermediate connection layer (ICL) of Po-T2T/LiF/Ag/HAT-CN/MoO3 is adopted to successfully develop monolithic tandem multicolor PeLEDs and WPeLEDs for the first time. The multicolor PeLEDs can achieve the best external quantum efficiency of 1.8% and the highest luminance of 4844 cd m−2. Besides, the red/green/blue (R/G/B) monolithic tandem WPeLED shows a standard white International Commission on Illumination coordinate of (0.33, 0.33) and achieves an extremely wide color gamut reaching  National Television Standards Committee of 130%. This study is the first to realize the standard R/G/B co-electroluminescence in a monolithic perovskite device and offers a feasible strategy for developing wide-color gamut perovskite displays.  相似文献   
27.
Li-rich layered oxides (LLOs) have been considered as the most promising cathode materials for achieving high energy density Li-ion batteries. However, they suffer from continuous voltage decay during cycling, which seriously shortens the lifespan of the battery in practical applications. This review comprehensively elaborates and summarizes the state-of-the-art of the research in this field. It is started from the proposed mechanism of voltage decay that refers to the phase transition, microscopic defects, and oxygen redox or release. Furthermore, several strategies to mitigate the voltage decay of LLOs from different scales, such as surface modification, elemental doping, regulation of components, control of defect, and morphology design are summarized. Finally, a systematic outlook on the real root of voltage decay is provided, and more importantly, a potential solution to voltage recovery from electrochemistry. Based on this progress, some effective strategies with multiple scales will be feasible to create the conditions for their commercialization in the future.  相似文献   
28.
Xu  Feihong  Zeng  Hui 《Wireless Personal Communications》2022,122(3):2035-2043
Wireless Personal Communications - Recently, Lee et al. (Sensors 20(14): 3983, 2020) proposed a certificateless aggregate arbitrated signature scheme CLAAS for IoT environments. Addobea et...  相似文献   
29.
Jin  Rong  Zeng  Kai  Jiang  Chuan 《Wireless Networks》2022,28(6):2437-2453
Wireless Networks - Friendly spectrum jamming is a flexible scheme to establish secure communications among heterogeneous wireless devices without the need of encryption. Previous works have...  相似文献   
30.
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