全文获取类型
收费全文 | 2303篇 |
免费 | 167篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 11篇 |
综合类 | 3篇 |
化学工业 | 971篇 |
金属工艺 | 20篇 |
机械仪表 | 42篇 |
建筑科学 | 64篇 |
矿业工程 | 6篇 |
能源动力 | 66篇 |
轻工业 | 472篇 |
水利工程 | 21篇 |
石油天然气 | 5篇 |
无线电 | 113篇 |
一般工业技术 | 311篇 |
冶金工业 | 92篇 |
原子能技术 | 5篇 |
自动化技术 | 269篇 |
出版年
2024年 | 4篇 |
2023年 | 47篇 |
2022年 | 187篇 |
2021年 | 226篇 |
2020年 | 75篇 |
2019年 | 97篇 |
2018年 | 81篇 |
2017年 | 81篇 |
2016年 | 102篇 |
2015年 | 89篇 |
2014年 | 95篇 |
2013年 | 168篇 |
2012年 | 143篇 |
2011年 | 182篇 |
2010年 | 158篇 |
2009年 | 117篇 |
2008年 | 102篇 |
2007年 | 85篇 |
2006年 | 78篇 |
2005年 | 53篇 |
2004年 | 37篇 |
2003年 | 39篇 |
2002年 | 42篇 |
2001年 | 20篇 |
2000年 | 9篇 |
1999年 | 21篇 |
1998年 | 23篇 |
1997年 | 11篇 |
1996年 | 10篇 |
1995年 | 10篇 |
1994年 | 8篇 |
1993年 | 10篇 |
1992年 | 7篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1970年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有2471条查询结果,搜索用时 15 毫秒
41.
Paolo Ria Antonio De Pascalis Anna Zito Silvia Barbarini Marcello Napoli Antonietta Gigante Gian Pio Sorice 《International journal of molecular sciences》2023,24(1)
Proteinuria is a broad term used to describe the pathological presence of proteins, including albumin, globulin, Bence-Jones protein, and mucoprotein in the urine. When persistent, proteinuria is a marker of kidney damage and represents a reliable predictor of the risk of progression of renal failure. Medical nutrition therapy is imperative for patients with proteinuria because it may slow the progression of renal disease. The aim of this review is to explore different nutritional approaches in the management of proteinuria and their influence on pathophysiological processes. As such, protein restriction is the main dietary intervention. Indeed, other management approaches are frequently used to reduce it regarding micro and macronutrients, but also the dietary style. Among these, the nutritional approach represents one of the most used and controversial interventions and the studies rarely take the form of randomized and controlled trials. With this work we aspire to analyze current clinical knowledge of how nutrition could influence proteinuria, potentially representing a useful tool in the management of proteinuric nephropathy. 相似文献
42.
Ilaria Andreana Manuela Malatesta Maria Assunta Lacavalla Federico Boschi Paola Milla Valeria Bincoletto Carlo Pellicciari Silvia Arpicco Barbara Stella 《International journal of molecular sciences》2023,24(1)
Muscular dystrophies are a group of rare genetic pathologies, encompassing a variety of clinical phenotypes and mechanisms of disease. Several compounds have been proposed to treat compromised muscles, but it is known that pharmacokinetics and pharmacodynamics problems could occur. To solve these issues, it has been suggested that nanocarriers could be used to allow controlled and targeted drug release. Therefore, the aim of this study was to prepare actively targeted poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) for the treatment of muscular pathologies. By taking advantage of the high affinity for carnitine of skeletal muscle cells due to the expression of Na+-coupled carnitine transporter (OCTN), NPs have been actively targeted via association to an amphiphilic derivative of L-carnitine. Furthermore, pentamidine, an old drug repurposed for its positive effects on myotonic dystrophy type I, was incorporated into NPs. We obtained monodispersed targeted NPs, with a mean diameter of about 100 nm and a negative zeta potential. To assess the targeting ability of the NPs, cell uptake studies were performed on C2C12 myoblasts and myotubes using confocal and transmission electron microscopy. The results showed an increased uptake of carnitine-functionalized NPs compared to nontargeted carriers in myotubes, which was probably due to the interaction with OCTN receptors occurring in large amounts in these differentiated muscle cells. 相似文献
43.
Manuel Porcar Silvia Ramos Amparo Latorre 《Journal of the science of food and agriculture》2007,87(14):2728-2731
BACKGROUND: The polymerase chain reaction (PCR) is a powerful tool that is being increasingly used for detection of transgenic DNA. PCR requires only a minute quantity of template, but sensitive and accurate testing requires DNA of sufficient purity and free from inhibitors such as plant polysaccharides. Several standard protocols are available for this purpose, but they usually involve several steps, imply destruction of the maize kernel, or are time‐consuming. Our aim was to develop a fast and simple extraction method to isolate a raw DNA‐containing solution from maize tissues suitable for use as a template in a PCR‐based detection assay with specific oligonucleotides directed to the identification of event MON810. RESULTS: The NaOH‐based DNA extraction method we report here is time‐saving (5 min) and can be used to isolate DNA‐containing solutions from a small maize leaf portion (down to 1 mg) or from a single overnight‐germinated kernel. PCR performed with selected primers yielded reproducible detection of transgenic DNA. CONCLUSION: The main advantages of the procedure are the quick extraction step, the possibility of non‐destructive testing of maize kernels, and the robustness of the PCR‐based detection, a consequence of the selection of MON810‐matching oligonucleotides yielding intense and highly specific amplicons. Copyright © 2007 Society of Chemical Industry 相似文献
44.
DNA barcoding as a new tool for food traceability 总被引:1,自引:0,他引:1
Andrea Galimberti Fabrizio De Mattia Alessia Losa Ilaria Bruni Silvia Federici Maurizio Casiraghi Stefano Martellos Massimo Labra 《Food research international (Ottawa, Ont.)》2013,50(1):55-63
Food safety and quality are nowadays a major concern. Any case of food alteration, especially when reported by the media, has a great impact on public opinion. There is an increasing demand for the improvement of quality controls, hence addressing scientific research towards the development of reliable molecular tools for food analysis. DNA barcoding is a widely used molecular-based system, which can identify biological specimens, and is used for the identification of both raw materials and processed food. In this review the results of several researches are critically analyzed, in order to exploit the effectiveness of DNA barcoding in food traceability, and to delineate some best practices in the application of DNA barcoding throughout the industrial pipeline. The use of DNA barcoding for food safety and in the identification of commercial fraud is also discussed. 相似文献
45.
Polysaccharides,oligosaccharides and nitrogenous compounds change during the ageing of Tempranillo and Verdejo sparkling wines 下载免费PDF全文
46.
Michela Pecoraro Stefania Marzocco Silvia Franceschelli Ada Popolo 《International journal of molecular sciences》2022,23(12)
Human epidermal growth factor receptor-2 (HER2) is overexpressed in up to 30% of breast cancer cases, causing a more aggressive tumour growth and poor prognosis. Trastuzumab, the humanized antibody targeted to HER2, increased the life expectancy of patients, but severe cardiotoxicity emerged as a long-term adverse effect. Clinical evidence highlights that Trastuzumab-induced cardiotoxicity drastically increases in association with Doxorubicin; however, the exact mechanisms involved remain incompletely understood. In order to analyse the molecular mechanisms involved and the possible adaptative responses to Trastuzumab and Doxorubicin treatment, in this study, H9c2 cardiomyoblasts were used. Results showed that Trastuzumab and Doxorubicin sequential administration in cardiomyoblast increased cytosolic and mitochondrial ROS production, intracellular calcium dysregulation, mitochondrial membrane depolarization, and the consequent apoptosis, induced by both Trastuzumab and Doxorubicin alone. Furthermore, in these conditions, we observed increased levels of Connexin43 phosphorylated on Ser368 (pCx43). Since phosphorylation on Ser368 decreases gap junction intracellular communication, thus reducing the spread of death signals to adjacent cells, we hypothesized that the increase in pCx43 could be an adaptative response implemented by cells to defend neighbouring cells by Trastuzumab and Doxorubicin sequential administration. However, the other side of the coin is the resulting conduction abnormalities. 相似文献
47.
Ricardo Cambraia Parreira Diana Paola Gmez‐Mendoza Itamar Couto Guedes de Jesus Rafael Pereira Lemos Anderson Kennedy Santos Cristiana Perdigo Rezende Henrique Csar Pereira Figueiredo Mauro Cunha Xavier Pinto Frank Kjeldsen Silvia Guatimosim Rodrigo Ribeiro Resende Thiago Verano‐Braga 《Proteomics. Clinical applications》2020,14(4)
48.
49.
Inés María López-Calleja Silvia de la CruzNicolette Pegels Isabel GonzálezRosario Martín Teresa García 《LWT》2014
Almond has been widely used in all sorts of food products, mostly due to its pleasant flavor and health benefits. However almonds can become an important health problem since they are responsible for triggering adverse immune responses in allergic individuals, and since they are present in many processed foods they are considered as a potential hidden allergen. Consequently, it's important for food processors and regulatory agencies to be able to ensure accurate labeling of foods to protect the safety of the public and to avoid expensive recalls. We propose a simple and highly sensitive approach to detect almond in a wide range of processed foods. The method consists of a real-time PCR assay targeting the gene encoding for the ITS1 in almond, using a nuclease (TaqMan) probe labeled with FAM and BBQ. Sensitivity of real time PCR was determined by analysis of raw and heat treated almond-wheat flour mixtures with a range of detection of 0.1–100,000 mg/kg. The assay was successfully trialed on a total of 214 commercial foodstuffs allowing the detection of trace amounts of almond down to the level of 0.1 mg/kg, and is therefore proposed as a ready-to-use analytical tool to trace almond allergens in foods. 相似文献
50.