Molecular Mechanisms of Fetal Tendon Regeneration Versus Adult Fibrous Repair

Tendinopathies are painful, disabling conditions that afflict 25% of the adult human population. Filling an unmet need for realistic large-animal models, we here present an ovine model of tendon injury for the comparative study of adult scarring repair and fetal regeneration. Complete regeneration of the fetal tendon within 28 days is demonstrated, while adult tendon defects remained macroscopically and histologically evident five months post-injury. In addition to a comprehensive histological assessment, proteome analyses of secretomes were performed. Confirming histological data, a specific and pronounced inflammation accompanied by activation of neutrophils in adult tendon defects was observed, corroborated by the significant up-regulation of pro-inflammatory factors, neutrophil attracting chemokines, the release of potentially tissue-damaging antimicrobial and extracellular matrix-degrading enzymes, and a response to oxidative stress. In contrast, secreted proteins of injured fetal tendons included proteins initiating the resolution of inflammation or promoting functional extracellular matrix production. These results demonstrate the power and relevance of our novel ovine fetal tendon regeneration model, which thus promises to accelerate research in the field. First insights from the model already support our molecular understanding of successful fetal tendon healing processes and may guide improved therapeutic strategies.     

Fertility-Sparing Approach in Women Affected by Stage I and Low-Grade Endometrial Carcinoma: An Updated Overview

Endometrial cancer (EC) is a deleterious condition which strongly affects a woman’s quality of life. Although aggressive interventions should be considered to treat high-grade EC, a conservative approach should be taken into consideration for women wishing to conceive. In this scenario, we present an overview about the EC fertility-sparing approach state of art. Type I EC at low stage is the only histological type which can be addressed with a fertility-sparing approach. Moreover, no myometrium and/or adnexal invasion should be seen, and lymph-vascular space should not be involved. Regarding the pharmaceutical target, progestins, in particular medroxyprogesterone acetate (MPA) or megestrol acetate (MA), are the most employed agent in conservative treatment of early-stage EC. The metformin usage and hysteroscopic assessment is still under debate, despite promising results. Particularly strict and imperious attention should be given to the follow-up and psychological wellbeing of women, especially because of the double detrimental impairment: both EC and EC-related infertility consequences.     

Two-step sinter-crystallization of K2O–CaO–P2O5–SiO2 (45S5-K) bioactive glass

Bioactive glasses and glass-ceramics (GCs) effectively regenerate bone tissue, however most GCs show improved mechanical properties. In this work, we developed and tested a rarely studied bioactive glass composition (24.4K₂O-26.9CaO-46.1SiO₂-2.6P₂O₅ mol%, identified as 45S5-K) with different particle sizes and heating rates to obtain a sintered GC that combines good fracture strength, low elastic modulus, and bioactivity. We analyzed the influence of the sintering processing conditions in the elastic modulus, Vickers microhardness, density, and crystal phase formation in the GC. The best GC shows improved properties compared with its parent glass. This glass achieves a good densification degree with a two-step viscous flow sintering approach and the resulting GC shows as high bioactivity as that of the standard 45S5 Bioglass®. Furthermore, the GC elastic modulus (56 GPa) is relatively low, minimizing stress shielding. Therefore, we unveiled the glass sintering behavior with concurrent crystallization of this complex bioactive glass composition and developed a potential GC for bone regeneration.     

The Cost of Irrigation Water Delivery: An Attempt to Reconcile the Concepts of Cost and Efficiency

This work discusses the conflict between two of the main objectives of the EU Water Framework Directive: cost recovery and economic efficiency in the use of irrigation water. In the Mediterranean region, this conflict is commonly embedded in a state of under-utilization of irrigation networks managed by Water User Associations (WUAs). This under-utilization arises from factors independent of farmers’ choice, such as water shortage, crises in the sector, and changes to the Common Agricultural Policy. This prevents the facilities from operating at the minimum average cost. This paper argues that farmers should not suffer this inefficiency, which results in them paying higher water prices to cover the costs of water supply. Indeed, the application of the Water Directive should be rethought, taking into account the specific problems related to irrigation in the Mediterranean region. Based on an econometric analysis of the costs of water distribution in a WUA in Sardinia (Italy), we propose a payment system based on two components. Primarily there is a fee related to the number of hectares under irrigation. In addition there is a fee that considers the intensity of irrigation: this component is to encourage farmers to save water. The results show that the proposed approach has some desirable effects, including higher rates of cost recovery and possibly a reduction in the use of groundwater. Finally, the proposed system is not overly expensive for the rest of the community, who must bear the costs of inefficiencies in the use of irrigation water that do not depend on choices of farmers.     

Life cycle assessment of different bioenergy production systems including perennial and annual crops

Energy crops are expected to greatly develop in a very short-term bringing to significant social and environmental benefits. Nevertheless, a significant number of studies report from very positive to negative environmental implications from growing and processing energy crops, thus great uncertainty still remains on this argument. The present study focused on the cradle-to-grave impact assessments of alternative scenarios including annual and perennial energy crops for electricity/heat or first and second generation transport fuels, giving special emphasis to agricultural practices which are frequently surprisingly neglected in Life Cycle Assessment studies despite a not secondary relevance on final outcomes. The results show that cradle-to-farm gate impacts, i.e. including the upstream processes, may account for up to 95% of total impacts, with dominant effects on marine water ecotoxicity. Therefore, by increasing the sustainability of crop management through minimizing agronomic inputs, or with a complementary use of crop resides, can be expected to significantly improve the overall sustainability of bioenergy chains, as well as the competitiveness against fossil counterparts. Once again, perennial crops resulted in substantially higher environmental benefits than annual crops. It is shown that significant amount of emitted CO₂ can be avoided through converting arable lands into perennial grasslands. Besides, due to lack of certain data, soil carbon storage was not included in the calculations, while N₂O emission was considered as omitted variable bias (1% of N-fertilization). Therefore, especially for perennial grasses, CO₂ savings were reasonably higher that those estimated in the present study. For first generation biodiesel, sunflower showed a lower energy-based impacts than rapeseed, while wheat should be preferred over maize for first generation bioethanol given its lower land-based impacts. For second generation biofuels and thermo-chemical energy, switchgrass provided the highest environmental benefits. With regard to bioenergy systems, first generation biodiesel was less impacting than first generation bioethanol; bioelectricity was less impacting than first generation biofuels and second generation bioethanol by thermo-chemical hydrolysis, but highly impacting than Biomass-to-Liquid biodiesel and second generation bioethanol through enzymatic hydrolysis.     

Shell-and-Tube Minichannel Condenser for Low Refrigerant Charge

The design of an innovative shell-and-tube heat pump condenser using 2 mm ID minichannels is presented. This condenser has been designed aiming at the minimization of the charge, which can be required by safety or environmental restrictions. Nevertheless, minichannels represent also a solution to the high-pressure challenge when using carbon dioxide as a refrigerant. The present prototype was realized for the use with propane in the framework of the European project SHERHPA, concerning the development of heat pumps working with natural fluids. Experimental data for heat transfer and pressure drop are reported in the present paper. The measurements have been obtained using refrigerant R22, which displays a temperature versus pressure saturation curve pretty close to the one of propane. The data have also been compared against a computational procedure for shell-and-tube heat exchangers design. The refrigerant charge has been computed by means of different void fraction correlations, showing that the expected charge is less than half the quantity required by a brazed plate condenser giving the same capacity.     

Benin 2025—Balancing Future Water Availability and Demand Using the WEAP ‘Water Evaluation and Planning’ System

In Benin, annual water availability per capita far exceeds the critical threshold of about 1,700 m³, but during the dry season, water scarcity occurs at the local scale. By modeling the water balance of the Ouémé–Bonou catchment with WEAP (Water Evaluation and Planning System), this study aimed at analyzing Benin’s future water situation under different scenarios of socio-economic development and climate change until 2025. The results show that the pressure on Benin’s water resources will increase, leading to greater competition for surface water. Furthermore, financial and technological constraints hinder a satisfactory development, and exploration of groundwater and reservoir resources. However, improvements are most needed, especially in rural areas. Decreasing inflows and groundwater recharge due to climate change aggravate this situation. Even though there are uncertainties and constraints concerning the model and input data, this study shows that the WEAP results offer a solid basis to assist planners in developing recommendations for future water resource management by revealing hot spots of action.     

Comparative analysis of the outdoor performance of a dye solar cell mini‐panel for building integrated photovoltaics applications

New generation photovoltaic (PV) devices such as polymer and dye sensitized solar cells (DSC) have now reached a more mature stage of development, and among their various applications, building integrated PVs seems to have the most promising future, especially for DSC devices. This new generation technology has attracted an increasing interest because of its low cost due to the use of cheap printable materials and simple manufacturing techniques, easy production, and relatively high efficiency. As for the more consolidated PV technologies, DSCs need to be tested in real operating conditions and their performance compared with other PV technologies to put into evidence the real potential. This work presents the results of a 3 months outdoor monitoring activity performed on a DSC mini‐panel made by the Dyepower Consortium, positioned on a south oriented vertical plane together with a double junction amorphous silicon (a‐Si) device and a multi‐crystalline silicon (m‐Si) device at the ESTER station of the University of Rome Tor Vergata. Good performance of the DSC mini‐panel has been observed for this particular configuration, where the DSC energy production compares favorably with that of a‐Si and m‐Si especially at high solar angles of incidence confirming the suitability of this technology for the integration into building facades. This assumption is confirmed by the energy produced per nominal watt‐peak for the duration of the measurement campaign by the DSC that is 12% higher than that by a‐Si and only 3% lower than that by m‐Si for these operating conditions. Copyright © 2013 John Wiley & Sons, Ltd.     

Rawseeds ground truth collection systems for indoor self-localization and mapping

A trustable and accurate ground truth is a key requirement for benchmarking self-localization and mapping algorithms; on the other hand, collection of ground truth is a complex and daunting task, and its validation is a challenging issue. In this paper we propose two techniques for indoor ground truth collection, developed in the framework of the European project Rawseeds, which are mutually independent and also independent on the sensors onboard the robot. These techniques are based, respectively, on a network of fixed cameras, and on a network of fixed laser scanners. We show how these systems are implemented and deployed, and, most importantly, we evaluate their performance; moreover, we investigate the possible fusion of their outputs.     

Reliable indirect competitive ELISA used for a survey of ochratoxin A in green coffee from the North of Paraná State, Brazil.

The performance of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) for ochratoxin A (OTA) detection was evaluated in a comparative study with high-performance liquid chromatography (HPLC) analysis using 68 freshly harvested coffee samples from the North of Paraná State, Brazil. The anti-OTA mAb showed high specificity and low cross-reactivity with OTA analogues (OTB and OTalpha), but cross-reacted with OTC. This ic-ELISA showed a detection limit of 3.75 ngg-1 sample, when compared to 0.80 ngg-1 by HPLC, with an ic-ELISA/HPLC correlation coefficient of 0.90. As regards OTA analysis of these coffee samples, natural contamination was detected in 10 samples (14.7%) by both methods, where the ic-ELISA values (range 3.9-7.3 ngg-1) were 1.1 to 1.6-fold higher than HPLC data (2.7-4.7 ngg-1). Five samples (7.4%) were OTA positive (range 0.84-1.30 ngg-1) only by HPLC assay, probably due to the higher detection limit reached by ic-ELISA. OTA was undetectable in 53 samples (77.9%) by both methods, while all positive samples (range 0.84-7.30 ngg-1) showed OTA levels lower than 8 ngg-1 (maximum limit recommended by the European Union). The matrix interference of green coffee was minimized by dilution of sample extracts before carrying out the ELISA assay. This mAb-based ic-ELISA can be effectively applied for OTA screening in coffee, because it is simple, sensitive and sample preparation is easy.