Do resources bolster coping and does coping buffer stress? An organizational study with longitudinal aspect and control for negative affectivity

Psychiatric workers facing redeployment completed questionnaire measures of stressors, resources (locus of control and perceived social support), coping, well-being, and negative affectivity, at baseline (N = 109) and 1 year later (loss of 7 participants). Regression analyses of the baseline data suggested that as stressors increased, so did avoidance coping, but less so for those high in internality or perceived social support. Problem-focused coping was bolstered by internality and emotion-focused coping by perceived social support. Other regression analyses, with a longitudinal aspect, suggested that stressors had a deleterious effect on well-being. Problem- and emotion-focused coping had beneficial effects, whereas avoidance coping had a (delayed) deleterious effect. These effects of coping were predominantly main and not buffering effects.     

Identification of residues in the cysteine-rich domain of Raf-1 that control Ras binding and Raf-1 activity

We have identified mutations in Raf-1 that increase binding to Ras. The mutations were identified making use of three mutant forms of Ras that have reduced Raf-1 binding (Winkler, D. G., Johnson, J. C., Cooper, J. A., and Vojtek, A. B. (1997) J. Biol. Chem. 272, 24402-24409). One mutation in Raf-1, N64L, suppresses the Ras mutant R41Q but not other Ras mutants, suggesting that this mutation structurally complements the Ras R41Q mutation. Missense substitutions of residues 143 and 144 in the Raf-1 cysteine-rich domain were isolated multiple times. These Raf-1 mutants, R143Q, R143W, and K144E, were general suppressors of three different Ras mutants and had increased interaction with non-mutant Ras. Each was slightly activated relative to wild-type Raf-1 in a transformation assay. In addition, two mutants, R143W and K144E, were active when tested for induction of germinal vesicle breakdown in Xenopus oocytes. Interestingly, all three cysteine-rich domain mutations reduced the ability of the Raf-1 N-terminal regulatory region to inhibit Xenopus oocyte germinal vesicle breakdown induced by the C-terminal catalytic region of Raf-1. We propose that a direct or indirect regulatory interaction between the N- and C-terminal regions of Raf-1 is reduced by the R143W, R143Q, and K144E mutations, thereby increasing access to the Ras-binding regions of Raf-1 and increasing Raf-1 activity.     

Differential regulation by estrogens of growth and prolactin synthesis in pituitary cells suggests that only a small pool of estrogen receptors is required for growth

PR1 cells are a prolactin (PRL)-secreting cell line derived from a pituitary lactotroph tumor found in 17beta-estradiol-treated Fischer 344 rats. We examined the effect of estrogen on cell proliferation and PRL synthesis under various culture conditions. Estrogen, at extremely low concentrations, induces cell proliferation in this cell line, whereas antiestrogen inhibits proliferation. Interestingly, the proliferation response is much more sensitive than the PRL response because 0.01 pM estradiol or diethylstilbestrol induces half-maximal growth induction [ approximately 0.1% estrogen receptor (ER) occupancy is required], whereas 0.01 nM concentration is required for half-maximal PRL induction ( approximately 50% ER occupancy is required). The proliferation response is not as sensitive to antiestrogen as the PRL response, because 10 nM concentration of the pure antiestrogen ICI 182,780 could not inhibit 1 nM estradiol- or diethylstilbestrol-induced proliferation. The same concentration of ICI 182,780 decreased PRL secretion to 1% of estradiol- or diethylstilbestrol-induced prolactin secretion suggesting a possible dichotomy of ER control of proliferation and PRL synthesis. The Kd of ER binding in these cells is about 3 x 10(-11) M. These results with the PR1 cells extend previous studies in other estrogen- regulated systems and suggest that only a small pool of ER is required for cell proliferation in contrast with the regulation of expression of specific genes. They also raise questions as to how a dimeric receptor functions when only one ligand site is occupied or when both an estrogen and an antiestrogen occupy one dimer.     

A microcontinuum analysis of the self propulsion of the spermatozoa in the cervical canal

The hydrodynamics of the self propulsion of a spermatozoa, swimming through the mucus filling the cervical channel, is investigated. The mucus is modeled as a micropolar fluid and the spermatozoa as a 2-dimensional sheet swimming at low Reynolds number between two rigid walls. The wavelengths of the propulsive waves passing down the sheet are assumed to be very large compared to the channel spacing, but the amplitude of the propulsive waves is arbitrary. Expressions for the propulsive velocity and the energy expended by the swimming sheet are obtained in terms of various parameters involved. The results are elaborated through graphs. It is found that both the propulsive velocity and the rate of working by the sheet increase as the value of the micropolar parameters $\text{N}$ increases and that of $\text{L}$ decreases.     

Iterative method of realisation of linearly separable switching functions

The minterms of the switching function expressed as decimal numbers are first partitioned into different groups according to the number of true inputs. An order of the weights and their signs can now be found easily. Secondary and higher orderings can also be found partially. An iteration procedure is next started by assigning values to the weights until a contradiction is reached. A new assignment is then chosen, guided by the nature of the contradiction, and this process is continued until the realisation is achieved. An example is worked out.     

Appendicectomy: an assessment of the advisability of stump invagination

Seven hundred and thirty-two cases of appendicectomy performed over a period of 5 years are reviewed to compare the incidence of complications and the length of the postoperative stay in hospital, depending on whether the appendix stump was simply ligated or was invaginated by purse string suture following ligation. No detrimental effects are noted following simple ligation, whereas patients who had stump invagination remained in hospital on average more than a day longer, mainly owing to a higher incidence of wound infection (16 per cent as against 6 per cent).     

Near-optimal control using reduced-order models

Two theorems have been proved demonstrating that the tracking properties of low-order models obtained using the theory of partial realisations are unchanged under state or output feedback. It follows, therefore, that an optimal feedback law for the reduced-order model can be used for near-optimal control of the high-order system with similar tracking of the output as for the open-loop case.     

n-GaAs Schottky diodes metallized with Ti and Pt/Ti

The electrical characteristics and interdiffusion in n-GaAs Schottky diodes containing Ti and Pt/Ti have been studied using I-V, C-V, X-ray diffraction and Rutherford backscattering measurements. Thermal aging of the diodes was carried out in vacuo at 350 and 500°C. The Ti/n-GaAs diodes show near-ideal forward I-V characteristics with the ideality parameter $\text{n ? 1·03}$ and the barrier height φ_B ～ 0·84V. C-V data yield the same φ_B. No interdiffusion was observed at 350°C. At 500°C, TiAs forms but the φ_B remains unchanged. Pt/Ti/n-GaAs diodes behave “Pt-like” upon aging at 500°C with φ_B ～ 0·9V and n ～ 1·1. The TiAs formed at 500°C is ineffective in preventing a subsequent Pt/GaAs interaction which leads to a layered arrangement of reaction products consisting of Pt₃Ga/TiAs/PtAs₂/n-GaAs.