Binding of tissue plasminogen activator to endothelial cells. The effect on functional properties. Localization of a ligand in the B-chain of tPA

The binding of 125I-labelled tissue plasminogen activator (tPA), the tPA A- or B-chain to endothelial cells (EC) were studied in suspensions of cultured human umbilical vein EC (HUVEC) or immortalized microvascular EC (HMEC). By determinations of the concentration-dependent binding it was shown that both the A-chain and the B-chain, which were isolated after partial reduction of two-chain tPA, contain ligands for binding to EC. The affinity for the B-chain was much higher than for the A-chain according to Scatchard analysis (Kd 24 and 515 nM, respectively), whereas the number of binding sites was higher for the A-chain than for the B-chain (Bmax 8 x 10(5) and 1.2 x 10(5), respectively). There were no cross interactions between the A- and B-chains and their binding sites. The binding of tPA to EC induced an almost 100-fold increase of the activation rate when compared to the same amount of enzyme in free solution, which in contrast to the fibrin-induced stimulation was not inhibited by antibodies against fibrin. The enzymatic activity of the B-chain was much less affected by the association to the cells. Both tPA and the tPA B-chain were largely protected against inhibition by an excess plasminogen activator type-1 (PAI-1) when bound to EC, whereas the same amount of free tPA was totally inactivated. The competition studies strongly indicated that an N-terminal segment in the B-chain, AKHRRSPGER, may be the ligand part of the B-chain. It is interesting to note that this polypeptide segment also participates in a binding site for PAI-1, necessary for effective inhibition. This implies a possible competition between PAI-1 and a tPA-receptor for binding of tPA. High molecular weight urokinase had no quenching effect on the binding of the B-chain to EC.     

Designing circuits with partial scan

In this scan design methodology, only selected faults are targeted for detection. These faults are those not detected by the designer's functional vectors. The test generator decides exactly which flip-flops should be scanned using one of two methods. In the first method, all possible tests are generated for each target fault, and the set of tests requiring the fewest flip-flops is selected. In the second method, only one test is generated for each fault, and the use of flip-flops is avoided as much as possible during test generation. Examples of actual VLSI circuits show a savings of at least a 40% in full-scan overhead     

Landmark-based spatial memory in birds (Parus atricapillus and Columba livia): The use of edges and distances to represent spatial positions.

Birds' use of landmarks to identify spatial locations was examined. Black-capped chickadees (Parus atricapillus) and pigeons (Columba livia) searched for hidden food on a 120-cm–2 tray. In each task the target was near an edge with a nearby landmark. On occasional unrewarded tests the landmark was either left in its usual position, shifted parallel to the edge, shifted perpendicular to the edge, or shifted diagonally. On diagonal landmark shifts the birds shifted their searching more in the parallel direction than in the perpendicular, which violates the predictions of the vector sum model (K. Cheng, 1989). In some cases the birds maintained their searching at a constant perpendicular distance under all landmark shifts. This suggests that perpendicular distance to an edge forms an element in determining where to search on the basis of landmarks. Chickadees and pigeons performed similarly, which suggests similarity in the way they encode locations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

阶跃信号突变点检测的小波分析法

文章运用小波理论这种新的数学工具,对于由基本平稳的慢变信号和阶跃型突变信号线性组合而构成的这类信号,提出了一种以连续小波变换为主体的检测和分析方法。理论以及实例分析表明,这种方法对于阶跃型突变信号的检侧,是颇为有效的。     

Design, modeling, and hardware correlation of a 3.2Gb/s/pair memory channel

With the rapid advance of silicon process technology, it is now possible to design input/output (I/O) circuits that operate at multigigabit data rates. As a result, accurate modeling and analysis of high-speed interconnect systems is essential to optimize the performance of the overall system. This paper describes the interconnect design, modeling, simulation, and characterization methodologies that are essential to achieve multigigabit data rates. It focuses on the physical layer verification and hardware correlation of functional systems and silicon to ensure robust system operation over 3.2Gb/s data rate using conventional low-cost packaging and printed circuit board (PCB) technologies. In order to capture conductor and dielectric losses, as well as other high-frequency effects of three-dimensional structures, accurate measurement-based simulation techniques that directly incorporate frequency-domain parameters from measurement or electromagnetic solver parameters into circuit simulation tools using fast Fourier transform (FFT) and bandlimiting windowing techniques are developed. Finally, simulation waveforms are correlated with prototypes at both component and system levels in both time and frequency domains.     

A3钢的工业大气腐蚀

在辽宁省工业城市进行 A3钢大气暴露试验。两年后测出不同地点腐蚀率。依据所记录的各试验点的气象数据和污染数据,讨论了 A3钢的大气腐蚀规律。     

近程地对地战术导弹的作用与问题

海湾战争中,“爱国者”导弹成功地拦截了“飞毛腿”导弹,给近程地对地战术导弹的作用、发展与装备方向提出了值得思考的问题。本文着重讨论在局部战争中,近程地对地战术导弹的作用、使用场合及其技术发展方向。     

大比例配用进口粉矿的烧结试验及生产

安钢烧结厂为应对原料条件的变化，就烧结配加进口粉矿进行了试验。以试验结果指导生产，顺利地实现了原料结构的改变，以及工艺参数的优化和生产指标的改善。     

结构化P2P网络中基于流言传播的负载均衡

提出了结构化P2P网络中利用结点间周期性的拓扑维护通信来夹带、传播消息进行负载均衡的方法，具体针对Chord系统从消息传播的速度和规模两个方面分析了该方法的性能，并利用仿真进行了验证。讨论了结论在其它形式网络中的推广和实现中的消息管理问题。     

UL简介与CCL认可

本文从六个方面对UL的基本知识作了一个简要的概述及CCL认可过程的介绍,希望能为业界同仁提供一个参考方向。