Chemical composition and antimicrobial activity of Vitex agnus-castus L. fruits and leaves essential oils

The following study deals with the chemical composition, antimicrobial activity of essential oils of Vitex agnus-castus L. and their main constituents in vitro and in vivo. The main compounds in the oil of unripe fruits were sabinene (17.8%) and 1,8-cineole (17.5%), while in the oil of the ripe fruits dominant compounds were 1,8-cineole (16.3%) and sabinene (13.4%). The leaves oil contained an abundance of 1,8-cineole (22.0%), as well. All of the oils tested were rich sources of α-pinene (12.2%, 9.4% and 9.4%, respectively). Antimicrobial activity was tested using bacterial and fungal strains by the microdilution method. Using the same technique 1,8-cineole and α-pinene showed very high antimicrobial potency as well. As 1,8-cineole was the predominant constituent of the oils, we have chosen to test it further in an in vivo experiment. Randomly chosen apples were treated with 1,8-cineol solution and infected with Aspergillus niger in order to provoke Aspergillus rot in apples. Disease incidence was recorded.     

Chemical characterization of the medicinal mushroom Phellinus linteus (Berkeley & Curtis) Teng and contribution of different fractions to its bioactivity

Mushrooms are widely appreciated for their organoleptic qualities, being also recognized as good sources of bioactive compounds that provide antioxidant, antimicrobial and cytotoxic activities. Polysaccharides (including glucans) are often pointed out as the most bioactive compounds isolated from mushrooms, but other molecules such as triterpenoids, might also be highlighted for their bioactivity. In scientific research, when isolated compounds are used, potential synergistic effects might be lost. Accordingly, the bioactivity of Phellinus linteus was evaluated in selected fractions (polysaccharides, glucans and triterpenoids), as well as in the methanolic and ethanolic extracts. The best antioxidant and antibacterial activities were obtained with methanolic extract, while glucan and triterpenoid fractions gave the strongest antifungal activity. In contrast, ethanolic extract gave the best results in cytotoxic activity, indicating that the bioactive compounds present might act synergistically. The differentiated activity of P. linteus fractions and extracts could be useful to find antimicrobial, antioxidant and cytotoxic agents as alternatives to synthetic chemicals with application in agriculture, food industry or pharmacy.     

Supercritical carbon dioxide extraction of sesquiterpenes from valerian root

Supercritical carbon dioxide extraction of sesquiterpenes from valerian root was investigated. Extractions from cultivar Arterner züctung and two wild grown valerian subspecies were performed. The influence of extraction conditions on extraction yield and chemical composition of obtained extracts was investigated at temperatures from 40 °C to 50 °C and pressures from 10 MPa to 20 MPa. Chemical composition of obtained extracts was analyzed by GC/FID and GC/MS methods. The influence of particle size on extraction process was investigated at 40 °C and 10 MPa. The major constituents of supercritical extracts were valerianol, valerenal, bornyl acetate and kessanyl acetate. Optimal extraction conditions for the cyclopentanoid sesquiterepenes isolation, referred to the content of cyclopentanoid sesquiterpenes in extract, were found to be 50 °C and 15 MPa. Extract obtained from the cultivar was characterized by the higher content of cyclopentanoid sesquiterpenes, as well as valerenal comparing to extracts from wild grown species. At 40 °C and 20 MPa 19.6% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. At 40 °C and 10 MPa, 17.4% of cyclopentanoid sequiterpenes isolated with methanol can be extracted. However, the extract obtained with methanol was characterized by the high content (42.51%) of unwanted isovaleric as well as by the lower content of cyclopentanoid sequiterpenes than the extracts obtained with supercritical carbon dioxide. The process of supercritical extraction was influenced by particle size. In order to simulate the process, mathematical model on the secretory structure scale was derived. Results of the model showed good agreement with the experimental data.     

UV Effect on Human Anterior Lens Capsule Macro-Molecular Composition Studied by Synchrotron-Based FTIR Micro-Spectroscopy

Ultraviolet (UV) irradiation is an important risk factor in cataractogenesis. Lens epithelial cells (LECs), which are a highly metabolically active part of the lens, play an important role in UV-induced cataractogenesis. The purpose of this study was to characterize cell compounds such as nucleic acids, proteins, and lipids in human UV C-irradiated anterior lens capsules (LCs) with LECs, as well as to compare them with the control, non-irradiated LCs of patients without cataract, by using synchrotron radiation-based Fourier transform infrared (SR-FTIR) micro-spectroscopy. In order to understand the effect of the UV C on the LC bio-macromolecules in a context of cataractogenesis, we used the SR-FTIR micro-spectroscopy setup installed on the beamline MIRAS at the Spanish synchrotron light source ALBA, where measurements were set to achieve a single-cell resolution with high spectral stability and high photon flux. UV C irradiation of LCs resulted in a significant effect on protein conformation with protein formation of intramolecular parallel β-sheet structure, lower phosphate and carboxyl bands in fatty acids and amino acids, and oxidative stress markers with significant increase of lipid peroxidation and diminishment of the asymmetric CH₃ band.     

Antifungal Activity of the Essential oil of Thymus vulgaris L. and Thymol on Experimentally Induced Dermatomycoses

The in vivo evaluation of the toxicological and antifungal activity of the essential oil of Thymus vulgaris L. and its main component thymol was made on 2-month-old male Wistar rats. We examined the therapeutic potency against experimentally induced dermatomycoses in rats, using the most frequent dermatomycetes, Trichophyton mentagrophytes, T. rubrum, and T. tonsurans. The therapeutic efficacy of a 1% solution of the essential oil of Thymus vulgaris and thymol as well as the commercial preparation bifonazole was evaluated. During the 37-day observation period the oil-treated animals were cured.     

Monitoring for exposures to TENORM sources in Vojvodina region

TENORM are found in a wide variety of waste materials, some raw mineral ores and in some consumer products (in trace amounts) where molecules of radionuclides may be bound to specific minerals used in the manufacturing process and can result in increases in radiation exposures to workers and the public. The aim of this paper is to understand this problem and to develop effective ways to protect humans and the environment from harmful exposure to the radiation in TENORM materials in the Vojvodina region. The results of measurement of indoor radon concentration in schools and kindergartens and dose-rate and gamma-spectrometry measurements of the workplace with TENORM materials are presented.