Effect of BCD Plasma on a Bacteria Cell Membrane

Cell membrane rupture is considered to be one of the probable mechanisms for bacterial inactivation using barrier corona discharge (BCD) plasma. In this paper, the effect of the BCD plasma on the Escherichia coli (E. coli) bacteria cell wall was investigated through two analytical methods; Adenosine-5-triphosphate (ATP) assay and Atomic Force Microscopy (AFM). The ATP assay results indicate an increase in the ATP content of samples which were exposed to the BCD plasma. This implies the bacteria cell rupture. Moreover, AFM images confirm a serious damage of the bacteria cell wall under the influence of the bactericidal agents of the plasma.     

Effects of Boiling on the IgE-Binding Properties of Tropomyosin of Shrimp (Litopenaeus vannamei )

ABSTRACT:  The thermal stability and IgE binding of raw and boiled shrimp extracts and the tropomyosins (TM) have not been reported. In this study, we compare the stability of raw and boiled shrimp TM of  Litopenaeus vannamei  and evaluate how boiling may alter the allergenicity of  L. vannamei.  Extracts were prepared from raw and boiled shrimp and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis. The IgE-binding of the extracts was determined by western-blot and competitive inhibition enzyme-linked immunosorbent assay (iELISA). The TM was then purified from raw and boiled shrimp, the secondary structures analyzed by circular dichroism (CD) spectroscopy, and the IgE binding compared by slot blot analysis. The soluble protein content decreased and the higher molecular weight proteins increased in the extracts from boiled versus raw shrimp. Similar IgE binding characteristics were seen by extracts when using western blot analysis. Although iELISA results showed that extracts from raw shrimp bound higher IgE than extracts from boiled shrimp, dot-blot assay demonstrates higher IgE binding to purified TM from boiled shrimp than raw shrimp. The purified TM had a typical alpha-helical secondary structure and the stability of boiled TM was lower than that of raw TM. Extracts from boiled shrimp produce lower IgE binding than extracts from raw shrimp, which suggest that boiling can be used as a tool in attempting to reduce shrimp allergenicity. However, the purified TM from boiled shrimp, which shows enhanced IgE binding over that of raw shrimp, may be a more effective antigen in diagnosing shrimp allergy through immunoassay.     

Fault tolerant control using virtual actuator for continuous‐time Lipschitz nonlinear systems

In this brief, we extend the existing results on fault tolerant control via virtual actuator approach to a class of systems with Lipschitz nonlinearities to maintain the closed‐loop stability after actuator faults. This generalization is established by relying on the input‐to‐state stability properties of cascaded systems. The virtual actuator block, placed between faulty plant and nominal controller, generates useful input signals for faulty plant by using output signals of the nominal controller to guarantee the closed‐loop stability in the presence of actuator faults. This design problem is reduced to a matrix inequality that can be turned to an LMI by fixing a variable to a constant value and solving the resulting LMI feasibility problem. The proposed fault tolerant control method is successfully evaluated using a nonlinear system. Copyright © 2013 John Wiley & Sons, Ltd.     

An ultra-energy-efficient crosstalk-immune interconnect architecture based on multilayer graphene nanoribbons for deep-nanometer technologies

An ultra-energy-efficient interconnect structure based on multilayer graphene nanoribbon (MLGNR) interconnects for deep-nanometer technologies is proposed herein. First, a low-swing interconnect based on MLGNRs and high-performance interface circuits using carbon nanotube field-effect transistors (CNTFETs) is proposed. Then, an ultra-energy-efficient interconnect structure is obtained by actively shielding such low-swing lines. The structures under study are simulated comprehensively at the 7-nm technology node. The results indicate that the MLGNR interconnect is significantly more energy efficient than its multiwall carbon nanotube (MWCNT) counterpart in the low-voltage regime. Moreover, the proposed approach is superior to its MLGNR counterparts. The proposed structure leads to 86%, 75%, and 31% lower energy consumption over a length of 500 µm as compared with the typical, actively shielded, and low-swing MLGNR interconnects, respectively. Moreover, the impact of the ratio of the widths of the signal line to the shield line on the performance of the interconnects is evaluated. The energy consumption reduction achieved by the proposed approach is mostly preserved even when using minimum-width shield lines on wider signal lines to reduce the area overhead. Moreover, the impact of process variations on the performance of the interconnects is assessed using Monte Carlo simulations, demonstrating the robustness of the proposed approach.

     

A review on DNA interaction with synthetic phenolic food additives

Synthetic phenolic food additives occupy an important place in food industry. Among the various food additives butylated hydroxyanisol (BHA), tertiary butylhydroquinone (TBHQ), 2-tert-butyl-4-methylphenol (TBMP) and propyl gallate (PG) have potential to form molecular complexes with nucleic acid structure and have attracted recent attention for their prospective industrial utility. This review highlights the properties of these synthetic phenolic food additives and their interactions with calf thymus DNA (CT-DNA) reported by several research groups. Various analytical techniques were employed including absorbance and fluorescence spectroscopies, viscosity, voltammetry and thermodynamic studies to provide more details about binding mechanism of these food additives with DNA.     

A comparative study on physicochemical properties of hydroxyapatite powders derived from natural and synthetic sources

Hydroxy apatite (HA) is effectively used as a bioimplant material because it closely resembles bone apatite and exhibits good biocompatibility. So, in this research, HA powders were produced by calcinations of natural bones including human, bovine, camel and horse bones, and also via sol-gel method. Powders characterizations of natural HA and Synthetic HA were studied by X-ray powder diffraction (XRD), X-ray fluorescence (XRF), Fourier transform infrared (FTIR) and scanning electron microscopy (SEM), combined with transmission electron microscopy (ТЕМ). In order to verify the biocompatibility of these HA powders, MTT assay was applied. The XRD results showed that the HA powders were successfully produced by using different sources. Also, it was obvious from XRF analysis that the main components of them were Ca and P. Furthermore, it was seen that the size of particles was in the nanometric scale and they showed agglomerates consisting of numerous nanocrystals. FTIR spectra of all samples proved the presence of various CO ₃ ^2- , PO ₄ ^3- and OH^– groups in the powders. In addition, the MTT assay revealed that the cells proliferations in the presence of horse and human HA nanopowders were stimulated.     

The effect of renewable energy and urbanization on CO2 emissions: A panel data

Europe has experienced fast-paced urbanization development over the past three decades. This paper empirically investigates the long-run equilibrium relationships and causal relationships among urbanization, renewable energy consumption, and CO₂ emissions, and this is important for EU countries’ future sustainable development. DOLS and FMOLS approaches are used for the period 1992–2014 .

Granger causality results show that there is a unidirectional relationship from CO₂ emissions to urbanization, and there is no causality between renewable energy consumption and CO₂ emissions. The results have important implications for EU policymakers on the path toward a sustainable society. Urbanization can have negative impacts on the natural environment with the net effect being hard in EU countries.     

The renewable energy,CO2 emissions,and economic growth: VAR model

This paper provides an empirical analysis of CO₂ emissions and economic growth, renewable energy consumption, and energy consumption over the period 1975–2014 in Germany. This paper uses the autoregressive distributed lag (ARDL) approach of cointegration test and vector error-correction models. The unit root and cointegration tests show that the long-run relationship between CO₂ emissions and its determinants. The empirical results show that the findings do not support the environmental Kuznets curve between real GDP and CO₂ emissions. To estimate the shocks of renewable energy consumptions, the study applies the dynamic test of Impulse Response Function (IRF) under the VAR method. The increasing portion of renewable energy consumption in electricity generation would have no impacts on the environment. However, the hikes of renewable energy sources would incur more cost to electricity producers and shrivel up the growth of economies through the expansionary effect of industry’s consumption and private capital spending in the Germany’s economy.     

Safety assessment of nanoparamagnetic contrast agents with different coatings for molecular MRI

Despite the wide application of gadolinium as a contrast agent for magnetic resonance imaging (MRI), there is a serious lack of information on its toxicity. Gadolinium and gadolinium oxide (Gd-oxide) are used as contrast agents for magnetic resonance imaging (MRI). There are methods for reducing toxicity of these materials, such as core nanoparticles coating or conjugating. Therefore, for toxicity evaluation, we compared the viability of commercial contrast agents in MRI (Gd-DTPA) and three nanoparticles with the same core Gd₂O₃ and small particulate gadolinium oxide or SPGO (< 40 nm) but different coatings of diethyleneglycol (DEG) as Gd₂O₃-DEG and methoxy polyethylene glycol-silane (mPEG-silane: 550 and 2000 Dalton) as SPGO-mPEG-silane550 and SPGO-mPEG-silane2000, respectively, in the SK-MEL3 cell line, by light microscopy, MTT assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, and the LDH assay detecting lactate dehydrogenase activity. The viability values were not statistically different between the three nanoparticles and Gd-DTPA. The MTT and LDH assay results showed that Gd₂O₃-DEG nanoparticles were more toxic than Gd-DTPA and other nanoparticles. Also, SPGO-mPEG-silane2000 was more biocompatible than other nanoparticles. The obtained results did not show any significant increase in cytotoxicity of the nanoparticles and Gd-DTPA, neither dose-dependent nor time-dependent. Therefore, DEG and PEG, due to their considerable properties and irregular sizes (different molecular weights), were selected as the useful surface covering materials of nanomagnetic particles that could reveal noticeable relaxivity and biocompatibility characteristics.     

Effect of SiC on the corrosion resistance of electroless Cu–P–SiC composite coating

In this work, Cu–P–SiC composite coatings were deposited via electroless plating with the addition of sodium hypophoshite (NaH₂PO₂) as a reducing agent. The coating compositions deposited were determined by using energy dispersive X-ray spectroscopy (EDX). The surface morphology of the coatings that were analyzed using scanning electron microscopy (SEM) showed that SiC particles were uniformly distributed by virtue of surfactant addition and mechanical stirring. The anti-corrosion properties of Cu–P and Cu–P–SiC coatings in NaCl and HCl solutions were investigated by the weight loss and potentiodynamic polarization techniques. The results showed that the corrosion resistance of Cu–P–SiC coatings was superior to that of electroless Cu–P coatings and carbon steel substrates in various concentrations of NaCl and HCl solutions.