Heterogeneity of human papillomavirus DNA in a patient with Bowenoid papulosis that progressed to squamous cell carcinoma

Bowenoid papulosis (BP) of the genitalia, characterized by the histological findings of a squamous cell carcinoma, follows a largely benign clinical course. The detection of oncogenic human papilloma viruses (HPV) from BP points to an aetiological role of these viral infections. A 47-year-old man with multiple genital skin lesions was seen over a 10-year period with the diagnosis of BP. Recently, he attended again with a recurrent genital tumour that was diagnosed as squamous cell carcinoma. His genital lesions progressed and became polymorphic in appearance, from a wart-like tumour to a reddish invasive plaque. To screen for the presence of different HPV sequences from different skin lesions and to correlate each HPV type with distinct clinical manifestations, polymerase chain reaction and single-strand conformational polymorphism (PCR-SSCP) were performed. PCR-SSCP revealed the presence of several types of HPV from different genital lesions. Sequencing results disclosed that he had a mixed infection of HPV6b, HPV16, HPV18 and HPV33, respectively. Interestingly, the clinical findings were fairly well correlated with the oncogenic potential of HPV found from each lesion.     

Cloning and characterization of cDNA for human adenylate kinase 2A

Considerable genomic microdiversity has been reported previously among Helicobacter pylori isolates. We have constructed genome maps of four unrelated H. pylori strains (NCTC11637, NCTC11639, UA802 and UA861) using pulsed-field gel electrophoresis (PFGE) with NotI and NruI, hybridization with extracted PFGE DNA fragments and probing with 17 gene probes. These strains of H. pylori were compared with a fifth unrelated H. pylori strain NCTC11638 mapped previously. Considerable diversity in gene arrangement was evident among the five H. pylori maps, and no consistent gene clustering was found. The association of only four genes, katA (catalase gene), vacA (vacuolating cytotoxin gene), hpaA (a putative adhesin gene), and pfr (bacterial ferritin gene) were generally conserved within approximately the same 25% of the genome; however, the order of these genes also varied. Our study demonstrates that macrodiversity, i.e. variability in gene order, in addition to microdiversity, is a characteristic of the H. pylori genome.     

Characteristics of body-tied triple-gate pMOSFETs

Body-tied triple-gate pMOSFETs were fabricated using bulk Si wafers and characterized. Process steps to implement the devices are explained briefly. Device characteristics of the triple-gate pMOSFETs were compared with those of the conventional planar channel device. While maintaining low off-leakage currents and threshold voltages similar to those of planar pMOSFETs in the parallel arrayed 30 000 transistors, the body-tied triple-gate MOSFETs showed about 74 mV/dec of subthreshold swing (92 mV/dec for conventional devices) and a drain-induced barrier lowering of 34 mV/V (92 mV/V for conventional devices). It was also addressed that I/sub SUB//I/sub D/ of the body-tied triple-gate is lower than that of the planar channel device.     

Phase transformation of metastable beta Ti alloys with tweed structure

This paper presents the finding of a microstructural abnormality generated in the metastable phase of a supersaturated state, and compares the results with ordering behavior such as premartensitic transformation. A tweed structure consisting of the metastable phase was observed by transmission electron microscopy, and spot satellites inflicted by streaks were also observed in lattice diffraction. The present report also reviews a new phase transformation from the metastable phase caused by the processes of aging, sub-zero quenching, and induced strain or stress.     

Changes in natural killer cell activity and prostaglandin E2 levels during the progression of diethylnitrosamine-induced hepatocarcinogenesis in Fischer 344 rats

The sequential changes of natural killer cell (NK) activity and prostaglandin E2 (PGE2) during hepatocarcinogenesis induced by diethylnitrosamine (DEN) in male Fischer 344 rats were investigated. DEN at a concentration of 40 ppm was administered in drinking water for 10 weeks. At weeks 5, 10, 20 and 30, rats were autopsied and the development of glutathione S-transferase placental form positive foci (GST-P+ foci) at weeks 5 and 10 and hepatocellular tumors at weeks 20 and 30 were examined. The labeling index of bromodeoxyuridine (BrdU) an indicator of DNA synthesis, was also sequentially checked. GST-P+ foci were found to increase with age. Hepatocellular nodules increased until week 20, but by week 30 when the incidence of hepatocellular carcinoma was 100%, the incidence of nodules had decreased. BrdU positive cells also increased with age, and by week 30 when the incidence of hepatocellular carcinoma was 100%, the number of BrdU-positive cells had decreased. NK cell activity increased until week 10, but at week 20, was less than in the untreated control group. The level of PGE2 increased until week 5, but at week 10, levels were not significantly different from those seen in the untreated control group. On the basis of these results, we concluded that NK activity is closely related to the progression of hepatocarcinogenesis, but PGE2 levels show no significant change.     

On the performance of a novel quasi-synchronous trellis-coded CDMA system

We introduce a novel signal set defined over a signal space that consists of L (L/spl ges/2) orthogonal planes, and a quasi-synchronous trellis-coded code-division multiple-access (TC-CDMA) system based on it. The proposed scheme makes efficient use of the available processing gain to improve power and/or bandwidth efficiency for practical multiuser interference environments. Having a multiplanar signal constellation structure, the proposed scheme provides several options for a given required data rate, which makes it better adapted to dynamic channel conditions. Analytical bounds and simulation results indicate that at practical error rates and 2 b/s/Hz the proposed scheme is approximately 1.2 dB better than a TC-CDMA system based on 8-PSK, and 3 dB better at 3 b/s/Hz compared to TC-CDMA using 16-QAM. Additionally, the proposed system is approximately 1 dB better than a multicoded system using two signature sequences per user.     

Low-noise cryogenic amplifier based on the Josephson effect

It is shown that an amplifier based on f.m. modulation of the frequency generated by a Josephson junction can have a very low-noise figure, and that it can handle voltages as small as 10?12 V.     

An Analytical Framework for Imperfect DS‐CDMA Closed‐Loop Power Control over Flat Fading

This letter presents an analytical framework for a performance analysis of the imperfect direct‐sequence code division multiple access (DS‐CDMA) closed‐loop power control (CLPC) loop with loop delay, channel estimation error, and power control command bit error as the parameters under a Rayleigh flat fading environment. The proposed model is verified through a comparison between analytical results and simulation ones.