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81.
Prevalence of Clostridium botulinum in food raw materials used in REPFEDs manufactured in France 总被引:1,自引:0,他引:1
Carlin F Broussolle V Perelle S Litman S Fach P 《International journal of food microbiology》2004,91(2):141-145
Food raw materials used in refrigerated processed foods of extended durability (REPFEDs) manufactured in France were surveyed for Clostridium botulinum types A, B and E using PCR-Enzyme-linked Immunosorbent assay (PCR-ELISA) and mouse bioassay for detection respectively of cells and toxins in enrichment broth. Portions of 25 to 50 g of food were analysed. A total of 8 out of the 102 samples of fish and shellfish, 12 out of the 143 samples of meat and poultry, 1 out of the 62 samples of aroma, sauce and gravy, 4 out of the 25 samples of thickening agents, 3 out of the 26 samples of dehydrated dairy ingredients, and none of the 65 samples of spices, herbs and dehydrated mushroom were positive for C. botulinum in PCR-ELISA, i.e., 6.6% of all the samples tested. The 28 positive samples comprised 10 type A, 10 type B, 4 with both types A and B, and 4 undetermined by PCR typing. No sample positive for type E was detected. Of the 28 samples positive in PCR-ELISA, 15 were also positive in the mouse bioassay. The MPN count was between 1 and 3 C. botulinum/kg of food, which is similar to or in the lower range of values reported in the literature. 相似文献
82.
Membranes with various pore size, length, morphology and density have been synthesized from diverse materials for size-exclusion-based separation. An example is the sterilization of intravenous lines by exclusion of bacteria and viruses using polyvinylidene fluoride membranes with 0.1-microm-diameter pores. Chemically specific filtration has recently been addressed for small molecules. Nevertheless, specific bio-organism immobilization and detection remains a great technical challenge in many biomedical applications, such as decontamination or analysis of air and liquids such as drinking water and body fluids. To achieve this goal, materials with controlled pore diameter, length and surface chemistry are required. In this letter, we present the first functionalized silicon membranes and demonstrate their ability to selectively capture simulated bio-organisms. These extremely versatile and rigid devices open the door to a new class of materials that are able to recognize the external fingerprints of bio-organisms-such as size and outer membrane proteins-for specific capture and detection applications. 相似文献
83.
The buffy-headed marmoset (Callithrix flaviceps) is apparently the only predispersal herbivore of the seeds of Siparuna guianensis at the Caratinga Biological Station in southeastern Brazil. Both the fruit receptacles and the frutioles (seeds) of S. guianensis are relatively rich in nutrients such as carbohydrates, proteins, and lipids, but the receptacles contain high concentrations of benzylisoquinoline alkaloids. The latter presumably act as a qualitative chemical defense, impeding the access of potential predators to the alkaloid-poor frutioles. However, on ripening, the receptacle splits open, exposing the frutioles, which enables C. flaviceps to avoid the plant's chemical defenses effectively. Taking care to avoid contact with the receptacle, the marmosets pluck out the frutioles and ingest the seeds. Qualitative and quantitative changes (in particular a significant reduction in daily ranging) in the marmosets' behavior during the period when S. guianensis frutioles were accessible indicate that this was a preferred plant resource. This is the first record of the consumption of S. guianensis seeds by callitrichine monkeys (which are not known to be systematic seed eaters), despite the fact that both are widely distributed in the Neotropics. It is thus possible that the behavioral strategy observed here is a unique phenomenon resulting from a specific combination of factors, including the abundance of S. guianensis within the study area. The lack of other records may nevertheless be a result of insufficient sampling effort, in which case, the feeding strategies of callitrichines may have an important influence on the reproductive biology of S. guianensis in many areas. 相似文献
84.
Mechanical flexing of plastic substrates coated with thin film permeation barriers causes stress-induced cracks that may lead to device degradation. This phenomenon is of particular importance for organic light emitting diodes, an emerging display technology that can be implemented on flexible substrates but imposes stringent requirements on the barrier performance. We demonstrate a dry-etch-based method to highlight cracks in thin films of transparent materials and make them visible under a conventional optical microscope on samples in a neutral, relaxed position. This approach allows for rapid evaluation of the mechanical performance of thin film barriers on flexible substrates. 相似文献
85.
Dominique Decouchant Sonia Mendoza Gabriela Sánchez José Rodríguez 《Expert systems with applications》2013,40(11):4446-4462
Owing to the dynamic nature of collaborative environments, the software intended to support collaborative work should adapt itself to the different situations that may occur. This requirement is related to the concept of “context of use”, which has been considered as an important aspect in the design of interactive systems. Nevertheless, two main problems about this concept have been identified by current research in context-aware computing: (1) most of the studies have mainly focused on the context of a single user, so the context of multiple users involved in a common endeavor remains little explored, and (2) adaptability in context-aware systems generally takes into account a reduced number of contextual variables (mainly the user’s location and platform). In this paper, we firstly re-conceptualize the notion of “context of use”, in order to consider the main characteristics of collaborative environments. Based on this new notion, we then design and implement a framework that allows application developers to specify the adaptability of groupware systems in terms of the state of activities, roles, collaborators’ location, available resources, and other typical variables of working groups. This framework has been generalized from scenarios that highlight dynamic situations presented in real collaborative settings. Finally, we validate our proposal by a set of applications that are able to adapt their user interface and functionality, when significant changes are produced in the environment, the working group, and/or the used devices. 相似文献
86.
Study of the effect of vintage,maturity degree,and irrigation on the amino acid and biogenic amine content of a white wine from the Verdejo variety 下载免费PDF全文
87.
88.
Luisa Barba Gianmichele Arrighetti Sonia Calligaris 《European Journal of Lipid Science and Technology》2013,115(3):322-329
Crystallization and melting properties of triacylglycerols in extra virgin olive oil were studied by using synchrotron X‐ray diffraction (XRD) and differential scanning calorimetry (DSC). The phase transitions were monitored by cooling and heating the samples at 2°C/min from 60 to ?60°C and vice versa. Upon cooling, a first DSC endothermic peak was recorded at ?9.6°C followed by one at ?33.5°C. These thermal events were associated to the formation of two different structures: a triple‐chain length (3L) having a c parameter of about 58.38 Å and a quadruple chain length structure (4L) with a c parameter of about 89.99 Å, respectively. Both structures evidenced a cell packing arrangement ascribable to a β′ form. During heating, part of the metastable β′ crystals rearranged into the more thermodynamically stable β form. Then, upon further heating, the sequential melting of the two crystal structures was observed. The melting was completed at 10.7°C. Beside this interpretation of XRD data, a model considering a cell with a c parameter of about 170 Å and a hexagonal crystal system was proposed. Even if more research is needed to validate this approach, it allowed all XRD events recorded during the experiments to be described. See commentary by Chiavaro [p. 267–269], http://dx.doi.org/10.1002/ejlt.201200415 相似文献
89.
90.
The MPEG- 2 Advanced Audio Coder is the latest issue of the MPEG audio encoders/decoders family, whose most popular version is known as MP3. It gathers many of the latest highly efficient sound compression techniques in a quite classically structured coder. The main part is based on a Discrete Cosine Transform with variable resolution. The output from this filterbank is compressed by the combination of an adaptive bit allocation module, according to frequency subbands, and a set of noiseless Huffman codebooks. Bit allocation is controlled by a psychoa-coustic model which determines an audibility threshold for signal distortion in the frequency domain. This article intends to explain the ISO standard without replacing it, and also to be a general introduction to perceptual audio coding. 相似文献