全文获取类型
收费全文 | 6058篇 |
免费 | 377篇 |
国内免费 | 5篇 |
专业分类
电工技术 | 79篇 |
综合类 | 13篇 |
化学工业 | 1563篇 |
金属工艺 | 140篇 |
机械仪表 | 124篇 |
建筑科学 | 412篇 |
矿业工程 | 29篇 |
能源动力 | 156篇 |
轻工业 | 466篇 |
水利工程 | 51篇 |
石油天然气 | 8篇 |
无线电 | 458篇 |
一般工业技术 | 1254篇 |
冶金工业 | 347篇 |
原子能技术 | 38篇 |
自动化技术 | 1302篇 |
出版年
2024年 | 12篇 |
2023年 | 102篇 |
2022年 | 144篇 |
2021年 | 231篇 |
2020年 | 156篇 |
2019年 | 128篇 |
2018年 | 191篇 |
2017年 | 169篇 |
2016年 | 256篇 |
2015年 | 251篇 |
2014年 | 311篇 |
2013年 | 412篇 |
2012年 | 388篇 |
2011年 | 473篇 |
2010年 | 358篇 |
2009年 | 352篇 |
2008年 | 352篇 |
2007年 | 325篇 |
2006年 | 257篇 |
2005年 | 221篇 |
2004年 | 167篇 |
2003年 | 157篇 |
2002年 | 131篇 |
2001年 | 83篇 |
2000年 | 81篇 |
1999年 | 78篇 |
1998年 | 117篇 |
1997年 | 66篇 |
1996年 | 59篇 |
1995年 | 63篇 |
1994年 | 43篇 |
1993年 | 40篇 |
1992年 | 30篇 |
1991年 | 20篇 |
1990年 | 18篇 |
1989年 | 18篇 |
1988年 | 17篇 |
1987年 | 13篇 |
1986年 | 8篇 |
1984年 | 24篇 |
1983年 | 12篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 11篇 |
1975年 | 6篇 |
1974年 | 6篇 |
排序方式: 共有6440条查询结果,搜索用时 15 毫秒
991.
Hamidreza Mahdavi Huacheng Zhang Lauren K.Macreadie Cara M.Doherty Durga Acharya Stefan J.D.Smith Xavier Mulet Matthew R.Hill 《Nano Research》2022,(4):3533-3538
Porous liquids(PLs)offer the potential to combine the ready handling and mature industry status of liquid absorbents,with the high permanent porosity of metal o... 相似文献
992.
Norbert Eisenreich ThomasS. Fischer Gesa Langer Stefan Kelzenberg Volker Weiser 《Propellants, Explosives, Pyrotechnics》2002,27(3):142-149
In the past, Vieille's law and minor modifications of it described sufficiently the linear burning rate of gun propellants which governs the design of charges by interior ballistic simulations. Recent developments to increase the performance led to new gun concepts and innovative propellants. These are the electrothermal‐chemical gun, porous and foamed charges as well as formulations exhibiting a temperature independent burning. Vieille's law cannot fully meet experimental results in these cases. Approaches based on the heat flow equation in the solid energetic material give simplified formulas to extend the validity. These burning rate models have the ability to describe the experimentally determined burning behavior at least in a simplified or qualitative way. More sophisticated methods consider complex geometrical structures in the solid or take into account the actual progress in phase behavior and reaction kinetics of heterogeneous combustion. The dependence of the burning rate on initial temperature, on phase transitions, porous structure and gaseous reactions can be described. 相似文献
993.
Anna Masek Stefan Cichosz Magorzata Piotrowska 《International journal of molecular sciences》2021,22(23)
The effects of plant-based extracts on the solar aging and antimicrobial properties of impregnated ethylene–norbornene (EN) copolymer and poly(lactic acid) (PLA) were investigated. In this study, the impregnation yield of polyolefin, lacking in active centers capable of phytochemical bonding, and polyester, abundant in active sides, was measured. Moreover, two different extracts plentiful in phytochemicals—thyme (TE) and clove (CE)—were employed in the solvent-based impregnation process. The effect of thymol and eugenol, the two main compounds embodied in the extracts, was studied as well. Interestingly, oxidation induction times (OIT) for the impregnation of EN with thyme and clove extracts were established to be, respectively, 27.7 and 39.02 min, which are higher than for thymol (18.4 min) and eugenol (21.1 min). Therefore, an aging experiment, mimicking the full spectrum of sunlight, was carried out to investigate the resistance to common radiation of materials impregnated with antioxidative substances. As expected, the experiment revealed that the natural extracts increased the shelf-life of the polymer matrix by inhibiting the degradation processes. The aging resistance was assessed based on detected changes in the materials’ behavior and structure that were examined with Fourier-transform infrared spectroscopy, contact angle measurements, color quantification, tensile tests, and hardness investigation. Such broad results of solar aging regarding materials impregnated with thyme and clove extracts have not been reported to date. Moreover, CE was found to be the most effective modifying agent for enabling material with antimicrobial activity against Escherichia coli to be obtained. 相似文献
994.
Amelie Lupp Blanca Ehms Ralf Stumm Johannes Gckeritz Christian Mawrin Stefan Schulz 《International journal of molecular sciences》2021,22(23)
Among the five somatostatin receptors (SST1–SST5), SST4 is the least characterized, which is in part due to the lack of specific monoclonal antibodies. We generated a knockin mouse model that expresses a carboxyl-terminal SST4-eGFP fusion protein. In addition, we extensively characterized the novel rabbit monoclonal anti-human SST4 antibody 7H49L61 using transfected cells and receptor-expressing tissues. 7H49L61 was then subjected to immunohistochemical staining of a series of formalin-fixed, paraffin-embedded normal and neoplastic human tissues. Characterization of SST4-eGFP mice revealed prominent SST4 expression in cortical pyramidal cells and trigeminal ganglion cells. In the human cortex, 7H49L61 disclosed a virtually identical staining pattern. Specificity of 7H49L61 was demonstrated by detection of a broad band migrating at 50–60 kDa in immunoblots. Tissue immunostaining was abolished by preadsorption of 7H49L61 with its immunizing peptide. In the subsequent immunohistochemical study, 7H49L61 yielded a predominant plasma membrane staining in adrenal cortex, exocrine pancreas, and placenta. SST4 was also found in glioblastomas, parathyroid adenomas, gastric and pancreatic adenocarcinomas, pheochromocytomas, and lymphomas. Altogether, we provide the first unequivocal localization of SST4 in normal and neoplastic human tissues. The monoclonal antibody 7H49L61 may also prove of great value for identifying SST4-expressing tumors during routine histopathological examinations. 相似文献
995.
996.
Petra M. Wise Paolo Neviani Stefan Riwaldt Thomas J. Corydon Markus Wehland Markus Braun Marcus Krüger Manfred Infanger Daniela Grimm 《International journal of molecular sciences》2021,22(23)
As much as space travel and exploration have been a goal since humankind looked up to the stars, the challenges coming with it are manifold and difficult to overcome. Therefore, researching the changes the human organism undergoes following exposure to weightlessness, on a cellular or a physiological level, is imperative to reach the goal of exploring space and new planets. Building on the results of our CellBox-1 experiment, where thyroid cancer cells were flown to the International Space Station, we are now taking advantage of the newest technological opportunities to gain more insight into the changes in cell–cell communication of these cells. Analyzing the exosomal microRNA composition after several days of microgravity might elucidate some of the proteomic changes we have reported earlier. An array scan of a total of 754 miRNA targets revealed more than 100 differentially expressed miRNAs in our samples, many of which have been implicated in thyroid disease in other studies. 相似文献
997.
Loredana-Mirela Lupu Pascal Wiegand Daria Holdschick Delia Mihoc Stefan Maeser Stephan Rawer Friedemann Vlklein Ebrahim Malek Frederik Barka Sascha Knauer Christina Uth Julia Hennermann Wolfgang Kleinekofort Andreas Hahn Günes Barka Michael Przybylski 《International journal of molecular sciences》2021,22(23)
Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (KD) of a protein–antibody complex is first determined by immobilizing an antibody or DNA-aptamer on an SPR chip. A proteolytic peptide mixture is then applied to the chip, and following removal of unbound material by washing, the epitope(s) peptide(s) are eluted and identified by MALDI-MS. The SPR-MS combination was applied to a wide range of affinity pairs. Distinct epitope peptides were identified for the cardiac biomarker myoglobin (MG) both from monoclonal and polyclonal antibodies, and binding constants determined for equine and human MG provided molecular assessment of cross immunoreactivities. Mass spectrometric epitope identifications were obtained for linear, as well as for assembled (“conformational”) antibody epitopes, e.g., for the polypeptide chemokine Interleukin-8. Immobilization using protein G substantially improved surface fixation and antibody stabilities for epitope identification and affinity determination. Moreover, epitopes were successfully determined for polyclonal antibodies from biological material, such as from patient antisera upon enzyme replacement therapy of lysosomal diseases. The SPR-MS combination was also successfully applied to identify linear and assembled epitopes for DNA–aptamer interaction complexes of the tumor diagnostic protein C-Met. In summary, the SPR-MS combination has been established as a powerful molecular tool for identification of protein interaction epitopes. 相似文献
998.
999.
M.Sc. Branca Delmonte Dr.-Ing. Sebastian Latz M.Sc. Jules Youmbi Prof. Dr.-Ing. Stefan Maas 《Bauphysik》2024,46(1):1-10
For decades efforts have been made to reduce the greenhouse gases emissions of buildings by reducing their energy demand with governmental regulations in Europe, pushing towards very low thermal transmittances (U-values) with ever thicker insulation layers for new buildings. However, there is no linear relationship between the insulation thickness and the heat losses. Therefore, above a certain thickness the consumption of buildings does not decrease significantly. Hereafter a life cycle analysis, including emissions before the building becomes operational is applied to evaluate the impact of the increasing thickness of components on the overall emissions. Publicly available product data sheets are used to compare four insulation materials under three scenarios. These analyses yield interesting results showing that energy-intensive insulation materials lead to a negative impact in the overall energy balance after a certain thickness. Even though there is not always a pronounced optimum insulation thickness, it is logical that further reductions in U-value for new buildings should hence be carefully evaluated. The results show that the optimal thickness is around 20 cm for most materials, while the important major savings come from the first 10 cm. 相似文献
1000.
Britta Schning Wolfgang H Ziegler Stefan Vieths Werner Baltes 《Journal of the science of food and agriculture》1996,71(4):475-482
Considering the known N-terminal amino acid sequence of the major apple allergen, a polymerase chain reaction (PCR) primer was selected to amplify cDNA encoding this protein. A single PCR product was obtained, cloned into Escherichia coli and subsequently sequenced. The missing 5′-end of the apple cDNA sequence was obtained by a 5′-RACE method. The cDNA sequence showed 72% identity with the coding region of one of the known isoforms of Bet v 1, the major allergen of birch pollen. The deduced amino acid sequence resulted in a 158-residue protein with a calculated molecular mass of 17·5 kDa and 63% amino acid sequence identity to Bet v 1. In addition, further protein alignments showed a high degree of identity with allergens from other tree pollens and some ‘pathogenesis-related proteins’ from food plants. According to international regulations the allergen was termed Mal d 1 for this protein, it being the first major allergen discovered and characterised in fruits of apple (Malus domestica). 相似文献