Laser-triangulation device for in-line measurement of road texture at medium and high speed

The knowledge of the friction coefficient between road and tyres is a very precious information to implement vehicle active control, especially considering the optimisation of the braking action. One of the most important parameters able to influence such a coefficient is the road texture (microtexture and macrotexture). Although different methods are now available to perform reliable texture measurements, no well-established techniques currently exist for measuring the pavement texture at medium-high speed with real time data analysis during the usual vehicle operations.This paper presents a method, based on two identical industrial laser-triangulation displacement transducers, allowing to get real time reliable road micro and macrotexture measurements during standard vehicle operations, even at medium and high speed. The presence of two transducers also allows to estimate the instantaneous vehicle speed, which is needed to obtain the road texture from the sensor time-histories. This means that the presented system can be considered as a stand-alone device able to give as an output the road texture (micro and macro) and also the vehicle speed without any other input. The paper also underlines the advantages of this method and its drawbacks. The method reliability is evidenced by some real time outdoor tests on the different road surfaces of the Pirelli test track and at different vehicle speeds.     

Magnetic circular dichroism in EELS: towards 10 nm resolution

We describe a new experimental setup for the detection of magnetic circular dichroism with fast electrons (EMCD). As compared to earlier findings the signal is an order of magnitude higher, while the probed area could be significantly reduced, allowing a spatial resolution of better than 40 nm. A simplified analysis of the experimental results is based on the decomposition of the mixed dynamic form factor S(q-->,q-->('),E) into a real part related to the scalar product and an imaginary part related to the vector product of the scattering vectors q--> and q-->('). Following the recent detection of chiral electronic transitions in the electron microscope the present experiment is a crucial demonstration of the potential of EMCD for nanoscale investigations.     

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Mycotoxins are fungal toxins produced by molds, which occur universally in food and feed derivatives, and are produced under certain environmental conditions in the field before harvest, post-harvest, during storage, processing, and feeding. Mycotoxin contamination is one of the most relevant and worrisome problem concerning food and feed safety because it can cause a variety of toxic acute and chronic effects in human and animals. In this review we report the use of mass spectrometry in connection with chromatographic techniques for mycotoxin determination by considering separately the most diffuse class of mycotoxins: patulin, aflatoxins, ochratoxin A, zearalenone, trichothecenes, and fumonisins. Although the selectivity of mass spectrometry is unchallenged if compared to common GC and LC detection methods, accuracy, precision, and sensitivity may be extremely variable concerning the different mycotoxins, matrices, and instruments. The sensitivity issue may be a real problem in the case of LC/MS, where the response can be very different for the different ionization techniques (ESI, APCI, APPI). Therefore, when other detection methods (such as fluorescence or UV absorbance) can be used for the quantitative determination, LC/MS appears to be only an outstanding confirmatory technique. In contrast, when the toxins are not volatile and do not bear suitable chromophores or fluorophores, LC/MS appears to be the unique method to perform quantitative and qualitative analyses without requiring any derivatization procedure. The problem of exact quantitative determination in GC/MS and LC/MS methods is particularly important for mycotoxin determination in food, given the high variability of the matrices, and can be solved only by the use of isotopically labeled internal standards or by the use of ionization interfaces able to lower matrix effects and ion suppressions. When the problems linked to inconstant ionization and matrix effects will be solved, only MS detectors will allow to simplify more and more the sample preparation procedures and to avoid clean-up procedures, making feasible low-cost, high-throughput determination of mycotoxins in many different food matrices.     

Can weathering improve the toughness of a fractured rock? A case study using the San Giacomo granite

The physical–mechanical behaviour of the weathered San Giacomo granite, Sardinia, has been investigated using a combination of physical–mechanical tests and microstructural analysis. Fieldwork and microscopy suggest a pervasive microfracture system developed on a pre-existing magmatic quartz anisotropy. Alteration indices, chemical analyses and mineralogy indicate that the yellow staining is produced by the circulation of oxygen-rich rainwater through microfractures in the rock, resulting in the oxidation of biotites. Apart from the obvious modification of the original grey colouring, the precipitation of Fe-oxides and hydroxides has welded fractures together, leading to the partial recovery of the physical–mechanical properties previously compromised due to microfracturing.     

Where does the money go? Economic flow analysis of construction projects

A new approach is introduced for analysing the economic flows in a construction project that will complement the existing methods. The main focus of the new method is to trace the flow of costs in the project and identify their spatial characteristics as well as who are the final recipients. This type of analysis highlights the flow of costs of the specific project in the local economy. It can be used for decision-making purposes from the project-commissioning party. Costs were divided into six categories, namely: labour, materials, energy, office overheads, taxes and personnel, and into two levels: local and national. Three case studies of stone and reinforced concrete structures were selected and analysed to test the application of this method. The case studies showed that this method and the representation by a Sankey diagram can provide useful insights regarding both the spatialized distribution of the cost of a project and the economic flows going to direct labour versus overhead and taxes. For future projects, local authorities or developers can select the most economically suitable strategy maximizing the income of the local community.     

Effect of thermostat and window opening occupant behavior models on energy use in homes

Existing dynamic energy simulation tools exceed the static dimension of the simplified methods through a better and more accurate prediction of energy use; however, their ability to predict real energy consumption is undermined by a weak representation of human interactions with the control of the indoor environment. The traditional approach to building dynamic simulation considers energy consumption as fully deterministic, taking into account standardized input parameters and using fixed and unrealistic schedules (lighting level, occupancy, ventilation rate, thermostat set-point). In contrast, in everyday practice occupants interact with the building plant system and building envelope in order to achieve desired indoor environmental conditions. In this study, occupant behavior in residential building was modelled accordingly to a probabilistic approach. A new methodology was developed to combine probabilistic user profiles for both window opening and thermostat set-point adjustments into one building energy model implemented in the dynamic simulation tool IDA Ice. The aim of the study was to compare mean values of the probabilistic distribution of the obtained results with a singular heating energy consumption value obtained by means of standard deterministic simulations. Major findings of this research demonstrated the weakness of standardized occupant behavior profile in energy simulation tools and the strengths of energy models based on measurements in fields and probabilistic modelling providing scenarios of occupant behavior in buildings.     

Reliability of parameter estimation in respirometric models

When modelling a biochemical system, the fact that model parameters cannot be estimated exactly stimulates the definition of tests for checking unreliable estimates and design better experiments. The method applied in this paper is a further development from Marsili-Libelli et al. [2003. Confidence regions of estimated parameters for ecological systems. Ecol. Model. 165, 127-146.] and is based on the confidence regions computed with the Fisher or the Hessian matrix. It detects the influence of the curvature, representing the distortion of the model response due to its nonlinear structure. If the test is passed then the estimation can be considered reliable, in the sense that the optimisation search has reached a point on the error surface where the effect of nonlinearities is negligible. The test is used here for an assessment of respirometric model calibration, i.e. checking the experimental design and estimation reliability, with an application to real-life data in the ASM context. Only dissolved oxygen measurements have been considered, because this is a very popular experimental set-up in wastewater modelling. The estimation of a two-step nitrification model using batch respirometric data is considered, showing that the initial amount of ammonium-N and the number of data play a crucial role in obtaining reliable estimates. From this basic application other results are derived, such as the estimation of the combined yield factor and of the second step parameters, based on a modified kinetics and a specific nitrite experiment. Finally, guidelines for designing reliable experiments are provided.     

Energy-saving strategies with personalized ventilation in cold climates

In this study the influence of the personalized supply air temperature control strategy on energy consumption and the energy-saving potentials of a personalized ventilation system have been investigated by means of simulations with IDA-ICE software. GenOpt software was used to determine the optimal supply air temperature. The simulated office room was located in a cold climate. The results reveal that the supply air temperature control strategy has a marked influence on energy consumption. The energy consumption with personalized ventilation may increase substantially (in the range: 61-268%) compared to mixing ventilation alone if energy-saving strategies are not applied. The results show that the best supply air temperature control strategy is to provide air constantly at 20 °C. The most effective way of saving energy with personalized ventilation is to extend the upper room operative temperature limit (saving up to 60% compared to the reference case). However, this energy-saving strategy can be recommended only in a working environment where the occupants spend most of their time at their workstation. Reducing the airflow rate does not always imply a reduction of energy consumption. Supplying the personalized air only when the occupant is at the desk is not an effective energy-saving strategy.     

Use of highly sensitive sublethal stress responses in the social amoeba Dictyostelium discoideum for an assessment of freshwater quality

In this work, the sensitivity of a battery of tests on the social amoeba Dictyostelium discoideum has been assessed within a freshwater toxicity study. The results obtained from the evaluation of survival and replication rate of D. discoideum were compared to those derived with a series of widely used tests for freshwater toxicity assessment, i. e. bioassays using Vibrio fischeri, Daphnia magna and Pseudokirchneriella subcapitata. The effects on sublethal endpoints, i.e. lysosomal membrane stability (LMS) and endocytotic rate, were analysed in conjunction with high-level endpoints to verify the potential to make a typical bioassay more sensitive. The field ecotoxicological investigation employing D. discoideum is part of a monitoring study assessing environmental quality of the Bormida River (Italy), subjected until recently to a chronic industrial pollution. The survey was carried out at several stations (upstream and downstream of a chemical factory outlet) in two different periods. In 2002, the results of chemical analyses performed on river water indicated no contamination. The ecotoxicological data obtained in this period showed that no evidence of biological effects was observed using V. fischeri and D. magna bioassays. In spite of the previous classical acute toxicity tests, significant differences in cell viability of D. discoideum were found. By analysing the effects measured on LMS and endocytotic rate, more relevant changes were observed for these sublethal stress biomarkers compared to survival. The chronic toxicity data showed significant changes in cell growth both of P. subcapitata and D. discoideum. Nevertheless, more sensitive and rapid responses were obtained when assessing the effects of exposure on D. discoideum. The chemical and ecotoxicological data obtained in 2006 indicated a full recovery of the quality of the river water (neither contamination nor toxicity found). Altogether, the results reported in this study underline that the use of a battery of biomarkers in conjunction with high-level endpoints may help follow the pollutant-induced stress syndrome in the organisms from early sublethal effects to starting mortality.