Two-beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures

To determine flow properties, namely, the velocity and angle of the flow in microstructured channels, an experimental realization based on fluorescence correlation spectroscopy is described. For this purpose, two micrometer-sized spatially separated volume elements have been created. The cross-correlation signal from these has been recorded and evaluated mathematically. In addition to previous results, two-beam cross-correlation allows for fast and easy determination of even small (down to 200 μm/s) flow velocities, as well as simultaneous measurement of diffusion properties of single dye molecules within a rather short detection time of 5-100 s and an error rate of less than 20%. The spatial flow resolution is around 1-2 μm, limited by the diameter of the volume element. Furthermore, vectorial flow data can be obtained and evaluated. A discussion of the theoretical background and an experimental verification of the theoretical results is performed. The feasibility of fast and easy data processing is shown if the flow time is the only desired information. Possible applications of this precise and simple method are the determination of transportation effects within artificial microstructures for CE and HPLC, fast chemical kinetics, and high-throughput screening.     

Secretarial attitudes towards word processors as a function of familiarity and locus of control

The purpose of the present study was to investigate dimensions of secretarial anxiety, eagerness, and curiosity with respect to word processing equipment. Such variables as familiarity, formal training, physical proximity to the equipment, degree of influence in the decision to acquire a word processor, and frequency of use were seen as potential predictors of such attitudes. Additionally, two trait-like dimensions were measured, locus of control and complexity. Questionnaires were sent to 408 secretaries employed at a mid-western university. The response rate was 59 per cent (n = 241). People who had not used word processing equipment were more anxious than those individuals who had. Moreover, frequency of use for people with experience was related to greater change in anxiety levels. While familiarity reduced anxiety, it also tended to reduce curiosity. Frequency of word processor use was related to several variables. For instance, frequency of use was related to how positively the person evaluated the processor as affecting their work capabilities. Heavy users felt that the manuals were less helpful. Internally controlled individuals were more eager, curious and less anxious, while cognitively complex individuals were more curious about word processors. The applied implications of the findings are discussed.     

Identification of the Sex Pheromone of the Currant Shoot Borer Lampronia capitella

Under an artificial light:dark cycle, females of Lampronia capitella were observed calling, with extended terminal abdominal segments, during the first 2 hr of the photoperiod. Extracts of terminal abdominal segments from females elicited large electroantennographic responses from male antennae. Gas chromatography with electroantennographic detection revealed three active peaks. Based on comparison of retention times and mass spectra of synthetic standards, these compounds were identified as (Z,Z)-9,11-tetradecadienol and the corresponding acetate and aldehyde. The electroantennographic activity of the four geometric isomers of all three compounds was investigated, and the respective (Z,Z)-isomer was found to be the most active in all cases. Aldehydes generally elicited larger antennal responses than alcohols, whereas acetates were the least active compounds. A subtractive trapping assay in the field, based on a 13:26:100 micrograms mixture of (Z,Z)-9,11-tetradecadienal, (Z,Z)-9,11-tetradecadienyl acetate, and (Z,Z)-9,11-tetradecadienol confirmed that all three compounds are pheromone components. Subtraction of (Z,Z)-9,11-tetradecadienol from the blend completely eliminated its attractiveness, whereas the other two-component blends showed reduced activity. This is the first pheromone identification from the monotrysian superfamily Incurvarioidea, confirming that the common pheromones among ditrysian moths (long-chain fatty acid derivatives comprising alcohols, acetates, and aldehydes with one or more double bonds) is not an autapomorphy of Ditrysia, but a synapomorphy of the more advanced heteroneuran lineages.     

Impact of natural organic matter (NOM) on freshwater amphipods

Natural organic matter (NOM) isolated from the eutrophic Sanctuary Pond (Point Pelee National Park, Canada) has an adverse impact on amphipod species (Gammarus tigrinus and Chaetogammarus ischnus from Lake Müggelsee, Germany, and Eulimnogammarus cyaneus, from Lake Baikal, Russia). Increases in amphipod mortality, changes in peroxidase activity and increases of heat shock protein (hsp70) expression were observed upon exposure to NOM. The highest resistance to the adverse impact of NOM was observed with the endemic Baikalian amphipod E. cyaneus. However, the mechanisms behind this finding remains obscure. If differences in the sensitivity of the hsp70 antibody may be excluded, different modes of action may be postulated: because the adverse impact of NOM may be caused by reactive oxygen species (ROS) and the NOM itself, the observed differences may be due to the action of ROS alone (with E. cyaneus) and a combination of both adverse modes of action (European species).     

The influence of a back support harness on spinal forces during sheep shearing

Previous research has classified the occupation of sheep shearing as heavy work where shearers flex their spine and hips for long periods of time, handle awkward loads and expend high amounts of energy. The aim of this research was to investigate the magnitude of spinal forces produced during the shearing phase of the work and to determine whether the use of a commercially available back support harness would reduce these forces. Following discussion on task complexity and risk of back injury with senior shearing instructors, three component tasks of the shearing phase were identified as posing high risk of injury and were prioritized for primary analysis. Although the dragging out of a sheep in preparation for shearing and an unexpected loss of animal control were also identified as being of high risk, technological and instrumentation difficulties precluded their analysis. Twelve experienced shearers were videotaped while shearing with and without the use of a back harness. Surface mounted retro-reflective markers placed on the trunk defined three linked segments: Pelvis, Lumbar and Head, Arms, and Upper Trunk (HAUT). A 3D, link segment, top down, inverse dynamics approach was used to describe the motion and to estimate forces involved during the identified tasks of shearing. The spinal force/time profiles of this sample of shearers demonstrated large compressive and shear forces for all three tasks that are close to the NIOSH and University of Waterloo action limits for compressive and shear forces respectively (McGill 1997, Yingling and McGill 1999, Marras 2000). The use of the back support harness reduced these forces by substantial and statistically significant amounts. This effect was consistent across all three tasks. The results of this study demonstrate the production of high levels of compressive and shear forces within the spine of shearers during the three shearing tasks studied and that the use of a back support harness can substantially reduce these forces. Therefore the use of a back harness may reduce the cumulative load on the spine during shearing thereby moderating damage to the articular structures. However it is not known whether the harness would protect the spine from a sudden or unexpected force.     

Parameterization-free active contour models with topology control

We present a novel approach for representing and evolving deformable active contours by restricting the movement of the contour vertices to the grid lines of a uniform lattice. This restriction implicitly controls the (re)parameterization of the contour and hence makes it possible to employ parameterization-independent evolution rules. Moreover, the underlying uniform grid makes self-collision detection very efficient. Our contour model is also able to perform topology changes, but – more importantly – it can detect and handle self-collisions at subpixel precision. In applications where topology changes are not appropriate, we generate contours that touch themselves without any gaps or self-intersections.     

Einsatzpotenziale von XML in Business-Intelligence-Systemen

This article describes the actual applications of XML in the context of Business- Intelligence-Systems (BI). The potentials and synergies of XML and BI will be emphasized.

1. XML applications can be found within all levels of BI-systems.
2. The application of core-standards will be introduced as well as the possibilities of BI specific-standards.
3. The discussion of the impact of XML for BI-systems follows along the dimensions: externalisation, integration, standardisation and rationalization.

     

Aerosol lidar intercomparison in the framework of the EARLINET project. 1. Instruments

In the framework of the European Aerosol Research Lidar Network to Establish an Aerosol Climatology (EARLINET), 19 aerosol lidar systems from 11 European countries were compared. Aerosol extinction or backscatter coefficient profiles were measured by at least two systems for each comparison. Aerosol extinction coefficients were derived from Raman lidar measurements in the UV (351 or 355 nm), and aerosol backscatter profiles were calculated from pure elastic backscatter measurements at 351 or 355, 532, or 1064 nm. The results were compared for height ranges with high and low aerosol content. Some systems were additionally compared with sunphotometers and starphotometers. Predefined maximum deviations were used for quality control of the results. Lidar systems with results outside those limits could not meet the quality assurance criterion. The algorithms for deriving aerosol backscatter profiles from elastic lidar measurements were tested separately, and the results are described in Part 2 of this series of papers [Appl. Opt. 43, 977-989 (2004)]. In the end, all systems were quality assured, although some had to be modified to improve their performance. Typical deviations between aerosol backscatter profiles were 10% in the planetary boundary layer and 0.1 x 10(-6) m(-1) sr(-1) in the free troposphere.     

Influence of thermophysical properties on pool boiling heat transfer of refrigerants

The correct prediction of the heat transfer performance of the boiling liquid within the evaporator of a refrigeration unit is one of the essential features for the successful operation of the whole unit. A theoretically consistent calculation method for the heat transfer coefficient α in nucleate boiling, which should be based on the physical phenomena connected with vapour bubbles growing, departing and sliding on the wall and with the interactions of bubbles and of neighbouring nucleation sites within the microstructure of the heating surface, does not yet exist, despite the increasing number of papers on the subject in the recent past. Instead, the predictive methods for α available at present are empirical or semiempirical, especially for heat transfer conditions relevant in practice. Many of these correlations have been established in the form of power laws in which the relative influences of the main groups of variables on α are treated by separate factors. One of these may stand for the influence of the thermophysical properties of the boiling liquid or these properties will be included in several of the factors.New experimental results are presented for pool boiling heat transfer from a single horizontal copper tube (8 mm OD) to HFC-refrigerants (R32, 125, 134a, 143a, 152a, 227ea) and hydrocarbons (propane, i-butane). The results are compared to experimental data from the literature, and methods are discussed, how to incorporate the data in semiempirical correlations to describe the influence of the thermophysical properties of the fluids on the heat transfer performance.