Cytopathological Outcomes of Knocking down Expression of Mitochondrial Complex II Subunits in Dictyostelium discoideum

Mitochondrial Complex II is composed of four core subunits and mutations to any of the subunits result in lowered Complex II activity. Surprisingly, although mutations in any of the subunits can yield similar clinical outcomes, there are distinct differences in the patterns of clinical disease most commonly associated with mutations in different subunits. Thus, mutations to the SdhA subunit most often result in mitochondrial disease phenotypes, whilst mutations to the other subunits SdhB-D more commonly result in tumour formation. The reason the clinical outcomes are so different is unknown. Here, we individually antisense-inhibited three of the Complex II subunits, SdhA, SdhB or SdhC, in the simple model organism Dictyostelium discoideum. Whilst SdhB and SdhC knockdown resulted in growth defects on bacterial lawns, antisense inhibition of SdhA expression resulted in a different pattern of phenotypic defects, including impairments of growth in liquid medium, enhanced intracellular proliferation of the bacterial pathogen Legionella pneumophila and phagocytosis. Knockdown of the individual subunits also produced different abnormalities in mitochondrial function with only SdhA knockdown resulting in broad mitochondrial dysfunction. Furthermore, these defects were shown to be mediated by the chronic activation of the cellular energy sensor AMP-activated protein kinase. Our results are in agreement with a role for loss of function of SdhA but not the other Complex II subunits in impairing mitochondrial oxidative phosphorylation and they suggest a role for AMP-activated protein kinase in mediating the cytopathological outcomes.     

密炼机动态消谐无功补偿设备

对某公司橡胶密炼机设备负荷进行实测分析,提出采用动态消谐无功补偿设备的解决方案,并介绍了该设备的特点、技术指标、校验以及补偿效果。     

浅谈恒温槽技术性能的测试方法

恒温槽作为热工类计量器具检定工作中不可缺少的主要配套设备,为了保证其技术性能的可靠性,需定期对恒温槽的技术性能进行测试.恒温槽技术性能的重要指标包括温度波动度和温场均匀性,它们会直接影响到检定结果的准确性.本文就是以制冷恒温槽为具体实例,介绍以二等铂电阻温度计为测试标准器,在0℃的时候,制冷恒温槽的温度波动度和温场均匀性,来叙述恒温槽技术性能的测试方法.     

基于P2P技术的媒体电信网

融合了P2P技术、利用电信运营商业务平台的媒体电信网(MTN)能够提供有服务质量保证、安全诚信的数字媒体业务。与现有客户端/服务器(C/S)和内容分发网络(CDN)架构的数字媒体系统相比,媒体电信网利用P2P技术,充分挖掘了用户终端资源,极大地降低网络和平台建设投资,具有低成本、高扩展性等独特优势。基于P2P技术的媒体电信网可以激活整个数字媒体产业价值链,具有广阔的市场前景。     

差压变送器在监测系统中的应用及故障处理

简单介绍了差压变送器的工作原理,设计出一套采集系统,利用北京鼎升力创R8000系列模块和差压传感器的配合来完成对数据的采集。并对R8000系列模块、差压变送器、引压管、变频干扰等在使用过程中的常见故障进行分析,提出解决方法。     

基于补偿特征图滤波算法去除光学相干层析造影成像俯视图的运动伪影

运动伪影是光学相干层析造影成像(OCTA)俯视图中的一个重要问题.提出一种补偿特征图像滤波(CEF)算法去除光学相干层析造影成像俯视图的运动伪影.首先,利用奇异值分解(SVD)将光学相干层析造影成像俯视图展开为一系列的特征图像;然后,利用高通特征图像和从第一特征图像中抽取得到的正交补偿积这两部分来重建恢复图像.实验结果...     

On centralized and distributed algorithms for minimizing data aggregation time in duty-cycled wireless sensor networks

We study the problem of minimizing data aggregation time in wireless sensor networks (WSNs) under the practical duty-cycle scenario where nodes switch between active states and dormant states periodically for energy efficiency. Under the protocol interference model, we show that the problem is NP-hard and present a lower bound of delay for any data aggregation scheme. To solve the problem efficiently, we then construct a routing tree based on connected dominator set and propose two aggregation scheduling algorithms, which are the centralized Greedy Aggregation Scheduling (GAS) and the distributed Partitioned Aggregation Scheduling (PAS), so as to generate collision-free transmission schedules for data aggregation in duty-cycled WSNs. To minimize the total delay, GAS tries to achieve maximal concurrent transmissions in each time-slot during each frame by using global information, while PAS leverages a network partition based strategy and local information to ensure the largest degree of channel reuse across space and time domains. Theoretical analysis indicates that each algorithm consumes at most \(O(R+\varDelta)\) frames and achieves nearly constant factor approximation on the optimal delay. Here R and \(\varDelta\) are the network radius and the maximum node degree, respectively. We also evaluate the practicability of our algorithms by extensive simulations under various network conditions and the results corroborate our theoretical analysis.     

ISP器件在高速通信系统中的应用

ISP器件具有集成度高、功能强、速度快、设计灵活和在系统逻辑重构等优点。为了改善数字通信系统发射和接收数据的同步性能，保证系统工作稳定、可靠，用ISP器件实现数字相关器。本文介绍了数字相关器的工作原理，电路设计方案和实现过程。针对通信系统中对速度的要求，在电路设计中采用了流水线技术，用Max＋PlusⅡ软件设计了十六位高速数字相关器。给出了十六位高速数字相关器顶层逻辑电路图及仿真波形图，由波形图可以看出，系统工作速度等于时钟频率。系统仿真正确后，用ISP器件实现了高速数字相关器。     

Biomimetic Hygroscopic Fibrous Membrane with Hierarchically Porous Structure for Rapid Atmospheric Water Harvesting

Sorption-based atmospheric water generation (SAWG) is a promising strategy to alleviate the drinkable water scarcity of arid regions. However, the high-water production efficiency remains challenging due to the sluggish sorption/desorption kinetics. Herein, a composite sorbent@biomimetic fibrous membrane (PPy-COF@Trilayer-LiCl) is reported by mimicking nature's Murray networks, which exhibits outstanding water uptake performance of 0.77–2.56 g g⁻¹ at a wide range of relative humidity of 30%–80% within 50 min and fast water release capacity of over 95% adsorbed water that can be released within 10 min under one sun irradiation. The superior sorption–desorption kinetics of PPy-COF@Trilayer-LiCl are enabled by the novel hierarchically porous structure, which is also the critical factor to lead a directional rapid water transport and vapor diffusion. Moreover, as a proof-of-concept demonstration, a wearable SAWG device is established, which can operate 10 sorption–desorption cycles per day in the outdoor condition and produce a high yield of clean water reaching up to 3.91 kg m⁻² day⁻¹. This study demonstrates a novel strategy for developing advanced solar-driven SAWG materials with efficient water sorption–desorption properties.