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991.
Repair of single-base mismatches formed in recombination intermediates in vivo was investigated in Chinese hamster ovary cells. Extrachromosomal recombination was stimulated by double-strand breaks (DSBs) introduced into regions of shared homology in pairs of plasmid substrates heteroallelic at 11 phenotypically silent mutations. Recombination was expected to occur primarily by single-strand annealing, yielding predicted heteroduplex DNA (hDNA) regions with three to nine mismatches. Product spectra were consistent with hDNA only occurring between DSBs. Nicks were predicted on opposite strands flanking hDNA at positions corresponding to original DSB sites. Most products had continuous marker patterns, and observed conversion gradients closely matched predicted gradients for repair initiated at nicks, consistent with an efficient nick-directed, excision-based mismatch repair system. Discontinuous patterns, seen in approximately 10% of products, and deviations from predicted gradients provided evidence for less efficient mismatch-specific repair, including G-A-->G-C specific repair that may reflect processing by a homologue of Escherichia coli MutY. Mismatch repair was > 80% efficient, which is higher than seen previously with covalently closed, artificial hDNA substrates. Products were found in which all mismatches were repaired in a single tract initiated from one or the other nick. We also observed products resulting from two tracts of intermediate length initiated from two nicks. 相似文献
992.
Various membranes, which have different materials and nominal molecular weight cut-offs (MWCO), were compared in terms of rejection of ibuprofen and removal of effluent organic matter (EfOM) from membrane bioreactor (MBR), because pharmaceutical compounds contain a potential risk and EfOM is the precursor of carcinogenic disinfection by-products when reusing for drinking water source. To provide equivalent comparison with respect to hydrodynamic condition, mass transfer parameter, J0/k ratio, was used. A tight-UF membrane with a molecular weight cut off of 8,000 daltons exhibited 25 approximately 95% removal efficiencies of ibuprofen with a molecular weight of 206 with and without presence of EfOM(MBR). EfOM(MBR) caused the reduction of ibuprofen removal efficiency for UF membrane. Rejection of EfOM(MBR) by UF and NF membranes ranged 29 approximately 47% and 69 approximately 86%, respectively. UF membrane could successfully remove ibuprofen at lower J0/k ratio range (< or = 1) in organic free water but could not efficiently reject ibuprofen with a relatively hydrophilic EfOM(MBR) (SUVA < or = 3). 相似文献
993.
Han-il Lee Je-Kwang Cho Kun-Seok Lee In-Chul Hwang Tae-Won Ahn Kyung-Suc Nah Byeong-Ha Park 《Solid-State Circuits, IEEE Journal of》2004,39(7):1164-1169
A fractional-N frequency synthesizer (FNFS) in a 0.5-/spl mu/m SiGe BiCMOS technology is implemented. In order to operate in a wide-band frequency range, a switched-capacitors bank LC tank voltage-controlled oscillator (VCO) and an adaptive frequency calibration (AFC) technique are used. The measured VCO tuning range is as wide as 600 MHz (40%) from 1.15 to 1.75 GHz with a tuning sensitivity from 5.2 to 17.5 MHz/V. A 3-bit fourth-order /spl Sigma/-/spl Delta/ modulator is used to reduce out-of-band phase noise and to meet a frequency resolution of less than 3 Hz as well as agile switching time. The experimental results show -80 dBc/Hz in-band phase noise within the loop bandwidth of 25 kHz and -129 dBc/Hz out-of-band phase noise at 400-kHz offset frequency. The fractional spurious is less than -70 dBc/Hz at 300-kHz offset frequency and the reference spur is -75 dBc/Hz. The lock time is less than 150 /spl mu/s. The proposed synthesizer consumes 19.5 mA from a single 2.8-V supply voltage and meets the requirements of GSM/GPRS/WCDMA applications. 相似文献
994.
Young Kyu Cho Duk Yong Yoon Byoung-Kee Kim 《Journal of the American Ceramic Society》2004,87(3):443-448
When NbC–30 wt% Co powder compact is sintered at various temperatures where NbC grains (with small amounts of Co) coexist with a liquid Co–NbC matrix, the NbC grains undergo a surface roughening transition with temperature increase and the grain growth changes from abnormal to normal growth. When sintered at 1400°C, the grains are polyhedral with sharp edges (and corners) and grow abnormally because their singular surfaces move by nucleation of surface steps. When sintered at 1600°C, the edges become round, indicating the surface roughening transition. The grains still grow abnormally, but their number density is larger than that at 1400°C because of the smaller surface step free energy. When sintered at 1820°C, the grains are nearly spherical, but the flat-surface segments still remain. The grain growth at this temperature is nearly normal because of very small surface step free energy. The surface roughening transition is reversed when a specimen initially sintered at 1820°C is heat-treated again at 1400°C, but some grains show transition shapes with nearly flat edges and slope discontinuities (shocks). 相似文献
995.
JH Cho DL Nicolae LH Gold CT Fields MC LaBuda PM Rohal MR Pickles L Qin Y Fu JS Mann BS Kirschner EW Jabs J Weber SB Hanauer TM Bayless SR Brant 《Canadian Metallurgical Quarterly》1998,95(13):7502-7507
The idiopathic inflammatory bowel diseases, Crohn's disease (CD) and ulcerative colitis (UC), are chronic, frequently disabling diseases of the intestines. Segregation analyses, twin concordance, and ethnic differences in familial risks have established that CD and UC are complex, non-Mendelian, related genetic disorders. We performed a genome-wide screen using 377 autosomal markers, on 297 CD, UC, or mixed relative pairs from 174 families, 37% Ashkenazim. We observed evidence for linkage at 3q for all families (multipoint logarithm of the odds score (MLod) = 2.29, P = 5.7 x 10(-4)), with greatest significance for non-Ashkenazim Caucasians (MLod = 3.39, P = 3.92 x 10(-5)), and at chromosome 1p (MLod = 2.65, P = 2.4 x 10(-4)) for all families. In a limited subset of mixed families (containing one member with CD and another with UC), evidence for linkage was observed on chromosome 4q (MLod = 2.76, P = 1.9 x 10(-4)), especially among Ashkenazim. There was confirmatory evidence for a CD locus, overlapping IBD1, in the pericentromeric region of chromosome 16 (MLod = 1.69, P = 2.6 x 10(-3)), particularly among Ashkenazim (MLod = 1.51, P = 7.8 x 10(-3)); however, positive MLod scores were observed over a very broad region of chromosome 16. Furthermore, evidence for epistasis between IBD1 and chromosome 1p was observed. Thirteen additional loci demonstrated nominal (MLod > 1.0, P < 0.016) evidence for linkage. This screen provides strong evidence that there are several major susceptibility loci contributing to the genetic risk for CD and UC. 相似文献
996.
The graft crosslinking polymerization of 4‐tert‐butylstyrene (tBS) and divinylbenzene (DVB) onto ethylene–propylene–diene (EPDM) was carried out in toluene by using benzoyl peroxide (BPO) as an initiator. The synthesized graft terpolymer, tBS‐EPDM‐DVB (PBED), was extracted with tetrahydrofuran (THF) into gel (called as PBED I) and sol, and then they were identified by infrared (IR) spectroscopy. The effects of solvent amount, molar ratio of DVB to tBS, EPDM content, initiator concentration, reaction temperature, and reaction time on the graft crosslinking polymerization were examined. Among them, solvent amount and molar ratio of DVB to tBS were the important factors for this reaction system. Maximum oil absorbency of PBED I was 84.0 g/g but its oil‐absorption kinetic rate was very low. Sol PBED can be reused as oil absorbent (named as PBED II) through photocrosslinking by ultraviolet light irradiation. Although the oil absorbencies of PBED II were lower than those of PBED I in most cases, their oil absorption kinetic rates were higher than oil absorbencies of PBED I. The highest value of oil absorbency of PBED II was 56.0 g/g. The thermal stability of PBED I was studied by TGA. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 2119–2129, 2002 相似文献
997.
We develop an efficient method for optimal timeslot scheduling in an interactive satellite multimedia network. We formulate the timeslot assignment problem as a binary integer programming (BIP) problem, where the throughput is maximized, and decompose this BIP problem into two sub-problems. With this decomposition, we promote the computational efficiency in finding the optimal solution of the original BIP problem. 相似文献
998.
999.
Two-phase boosted voltage generator for low-voltage DRAMs 总被引:1,自引:0,他引:1
Seong-Ik Cho Jung-Hwan Lee Hong-June Park Gyu-Ho Lim Young-Hee Kim 《Solid-State Circuits, IEEE Journal of》2003,38(10):1726-1729
A two-phase boosted voltage (V/sub PP/) generator circuit was proposed for use in gigabit DRAMs. It reduced the maximum gate-oxide voltage of pass transistor and the lower limit of supply voltage to V/sub PP/ and V/sub TN/, respectively, while those for the conventional charge-pump circuit are V/sub PP/+V/sub DD/ and 1.5 V/sub TN/ respectively. Also, the pumping current was increased in the new circuit. The newly proposed two-phase V/sub PP/ charge-pump circuit worked successfully at V/sub DD/ down to 0.8 V by eliminating the threshold voltage loss of the control pulse generator and was tested successfully in a 0.16-/spl mu/m test chip using triple-well CMOS technology. 相似文献
1000.