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61.
Lamarque M Tastet C Poncet J Demettre E Jouin P Vial H Dubremetz JF 《Proteomics. Clinical applications》2008,2(9):1361-1374
The Plasmodium falciparum food vacuole (FV) is a lysosome-like organelle where erythrocyte hemoglobin digestion occurs. It is a favorite target in the development of antimalarials. We have used a tandem mass spectrometry approach to investigate the proteome of an FV-enriched fraction and identified 116 proteins. The electron microscopy analysis and the Western blot data showed that the major component of the fraction was the FV and, as expected, the majority of previously known FV markers were recovered. Of particular interest, several proteins involved in vesicle-mediated trafficking were identified, which are likely to play a key role in FV biogenesis and/or FV protein trafficking. Recovery of parasite surface proteins lends support to the cytostomal pathway of hemoglobin ingestion as a FV trafficking route. We have identified 32 proteins described as hypothetical in the databases. This insight into FV protein content provides new clues towards understanding the biological function of this organelle in P. falciparum. 相似文献
62.
Barelli S Canellini G Thadikkaran L Crettaz D Quadroni M Rossier JS Tissot JD Lion N 《Proteomics. Clinical applications》2008,2(2):142-157
Protein oxidation mechanisms result in a wide array of modifications, from backbone cleavage or protein crosslinking to more subtle modifications such as side chain oxidations. Protein oxidation occurs as part of normal regulatory processes, as a defence mechanism against oxidative stress, or as a deleterious processes when antioxidant defences are overcome. Because blood is continually exposed to reactive oxygen and nitrogen species, blood proteomics should inherently adopt redox proteomic strategies. In this review, we recall the biochemical basis of protein oxidation, review the proteomic methodologies applied to analyse redox modifications, and highlight some physiological and in vitro responses to oxidative stress of various blood components. 相似文献
63.
64.
Jo B.-H. Van Lerberghe L.M. Motsegood K.M. Beebe D.J. 《Journal of microelectromechanical systems》2000,9(1):76-81
This paper describes a fabrication technique for building three-dimensional (3-D) micro-channels in polydimethylsiloxane (PDMS) elastomer. The process allows for the stacking of many thin (less than 100-μm thick) patterned PDMS layers to realize complex 3-D channel paths. The master for each layer is formed on a silicon wafer using an epoxy-based photoresist (SU 8). PDMS is cast against the master producing molded layers containing channels and openings. To realize thin layers with openings, a sandwich molding configuration was developed that allows precise control of the PDMS thickness. The master wafer is clamped within a sandwich that includes flat aluminum plates, a flexible polyester film layer, a rigid Pyrex wafer, and a rubber sheet. A parametric study is performed on PDMS surface activation in a reactive-ion-etching system and the subsequent methanol treatment for bonding and aligning very thin individual components to a substrate. Low RF power and short treatment times are better than high RF power and long treatment times, respectively, for instant bonding. Layer-to-layer alignment of less then 15 μm is achieved with manual alignment techniques that utilize surface tension driven self-alignment methods. A coring procedure is used to realize off-chip fluidic connections via the bottom PDMS layer, allowing the top layer to remain smooth and flat for complete optical access 相似文献
65.
This article presents the result of the current knowledge on the very high frequency electromagnetic radiation of lightning. A phenomenology analysis of the new results obtained from the study of triggered lightning in New Mexico (TRIP 82) and at Saint-Privat-d’ Allier in Haute-Loire, France (1983) is first described. The authors then present the spectral and temporal characteristics of the VHF/UHF radiation which can be applied to the electromagnetic compatibility of systems. From a synthesis of the experimental and theoretical contributions, they propose a reflection on the lightning initiation process and on the mechanisms generating lightning radiation. 相似文献
66.
67.
Stefan Kiefer Andrzej S. Murawski Jo?l Ouaknine Bj?rn Wachter James Worrell 《Formal Aspects of Computing》2012,24(4-6):671-678
Herman’s algorithm is a synchronous randomized protocol for achieving self-stabilization in a token ring consisting of N processes. The interaction of tokens makes the dynamics of the protocol very difficult to analyze. In this paper we study the distribution of the time to stabilization, assuming that there are three tokens in the initial configuration. We show for arbitrary N and for an arbitrary timeout t that the probability of stabilization within time t is minimized by choosing as the initial three-token configuration the configuration in which the tokens are placed equidistantly on the ring. Our result strengthens a corollary of a theorem of McIver and Morgan (Inf. Process Lett. 94(2): 79–84, 2005), which states that the expected stabilization time is minimized by the equidistant configuration. 相似文献
68.
Thirty-three children between 3 and 6 years of age were asked to climb four different types of safety barriers. Morphological and functional variables of the children, which were expected to influence climbing or passing through skills, were collected. The influence of those variables on children’s success rate and time to cross was tested. No barrier offered a total restraining efficacy. The horizontal bars barrier was crossed by 97% of the children. In the group of children that succeeded in crossing the four barriers, mean time to cross the most difficult barrier was 15 s. Age was the best predictor for success in crossing most barriers but morphology and strength were important predictors of time to cross. The influence of anthropometric variables in time to cross was dependent upon the characteristics of the barrier. A good design of safety barriers should consider children’s age, morphology and strength. 相似文献
69.
70.
Tae Jo Ko Jung Whan Park Hee Sool Kim Sun Ho Kim 《The International Journal of Advanced Manufacturing Technology》2007,32(7-8):739-746
Once a machining process is finished, an inspection process is carried out to check whether the part is within dimensional
tolerances. A coordinate measuring machine (CMM) is a general metrological device for assessment of dimensions on the shop
floor. It cannot be ignored, however, that CMM measurements require significant resources in operating time and cost, which
has led to many studies into on-machine measurement (OMM) systems. This study aims to develop an OMM system with a noncontacting
laser displacement sensing apparatus and a computer-aided design (CAD) model for ease of operation, improved operating speed,
and free form profiling. The system is composed of two software modules, one for sensor alignment with the machine tool and
the other for measurement based on CAD/CAM (computer-aided machining). Consequently, the system was verified on the shop floor
at a numerical control (NC) machining center. 相似文献