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71.
Braunschweig T Kaserer K Chung JY Bilke S Krizman D Knezevic V Hewitt SM 《Proteomics. Clinical applications》2007,1(3):264-271
Thyroid cancer is the most common endocrine neoplasm with multiple histologic subtypes, each associated with different treatments and outcomes. Differentiating benign neoplasms such as follicular adenomas from malignant entities such as follicular carcinomas and papillary carcinoma can be challenging. To define the proteomic profile of different thyroid tumors, we screened an antibody array of 330 features against five thyroid neoplasms: follicular adenoma, follicular carcinoma, papillary carcinoma, anaplastic carcinoma, and medullary carcinoma as well as normal thyroid epithelium. Eight candidate biomarkers; c-erbB-2, Stat5a, Annexin IV, IL-11, RARα, FGF7, Caspase 9, and phospho-c-myc were identified as differentially expressed on the antibody array, and validated with immunohistochemistry on tissue microarrays, with a total of 144 samples of the same variety of thyroid neoplasms. Analysis revealed c-erbB-2, Annexin IV, and Stat5a have potential clinical utility to differentiate follicular adenoma, follicular carcinoma, and papillary carcinoma from each other. By using an antibody array as a discovery platform and a tissue microarray as a first step in validation on a large number of specimens, we have identified new markers that have potential utility in the diagnosis of thyroid neoplasms. 相似文献
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73.
In feature-oriented programming (FOP) a programmer decomposes a program in terms of features. Ideally, features are implemented modularly so that they can be developed in isolation. Access control mechanisms in the form of access or visibility modifiers are an important ingredient to attain feature modularity as they allow programmers to hide and expose internal details of a module’s implementation. But developers of contemporary feature-oriented languages have not considered access control mechanisms so far. The absence of a well-defined access control model for FOP breaks encapsulation of feature code and leads to unexpected program behaviors and inadvertent type errors. We raise awareness of this problem, propose three feature-oriented access modifiers, and present a corresponding access modifier model. We offer an implementation of the model on the basis of a fully-fledged feature-oriented compiler. Finally, by analyzing ten feature-oriented programs, we explore the potential of feature-oriented modifiers in FOP. 相似文献
74.
JA Frank RE Hoffman JM Mann JD Crowe AR Hinman 《Canadian Metallurgical Quarterly》1981,245(3):264-266
From Dec 23, 1978, through Jan 31, 1979, an outbreak of five laboratory-confirmed cases and four clinical cases of measles occurred in a Vietnamese refugee population living in a single housing complex in Albuquerque, NM. The index cases were in two refugee siblings in whom measles was incubating on arrival in the United States. Despite spread through three subsequent generations of disease transmission within the Vietnamese population, there was no additional spread into the general Albuquerque population. Responsible factors included the age distribution of susceptible persons, the social isolation of the refugee population, and the physical structure of the housing complex. There is a need to identify the problem of imported measles in "ethnic islands" in need of vaccination. 相似文献
75.
In this paper, a particular class of nano-diamond films deposited by energetic species is described. Deposition is carried out using the direct-current glow-discharge (DC GD) deposition technique from a methane/hydrogen mixture. In this method, film growth occurs from energetic species being accelerated and incorporated into the film surface. The growth of the nano-diamond film occurs on top of a preferentially oriented graphitic precursor with its basal planes perpendicular to the substrate surface. The nano-diamond films consist of an agglomerate of diamond particles with particle sizes in the 3-5 nm range with amorphous grain boundaries. The hydrogen concentration in the graphitic precursor is only a few percent; however, it increases to ∼15-20 at.% in the nano-diamond film.From a microscopic perspective nano-diamond film and growth from energetic species is explained as a sub-surface process in terms of a four-step cyclic process. The DC GD-deposited nano-diamond films were comprehensively explored by a number of complementary techniques. The hydrogen content and its role in nano-diamond film formation were assessed. The experimental methods used in our studies comprise near-edge X-ray adsorption fine structure (NEXAFS) to prove the short-range coordination of the carbon films and indirectly their phase composition. The surface and grain boundary phase composition were investigated by a combination of electron energy loss spectroscopy (EELS) measured as a function of incident electron energy and hydrogen etching experiments. By transmission electron microscopy (TEM), the micro-structural evolution and their visualization were achieved. The density evolution of the films was determined by X-ray reflectivity (XRR). The hydrogen content and its distribution in the films were studied by secondary ion microscopy spectroscopy (SIMS) and elastic recoil detection (ERD). The hydrogen bonding was investigated by high-resolution electron energy loss spectroscopy (HREELS).Most likely, hydrogen is bonded within the amorphous grain boundaries and saturates the nano-diamond particles. The surface of the films is amorphous in nature. 相似文献
76.
A new generation of beam deflection technique is the basis for the development of electron beam (EB) technologies, which are not realisable with conventional methods. A high frequency 3D‐beam deflection makes possible multi‐field, multi‐pool and multi‐process technologies for EB welding und EB surface treatment, which result in a clear higher productivity and new combinations of properties. 相似文献
77.
Multitarget miss distance via optimal assignment 总被引:1,自引:0,他引:1
Hoffman J.R. Mahler R.P.S. 《IEEE transactions on systems, man, and cybernetics. Part A, Systems and humans : a publication of the IEEE Systems, Man, and Cybernetics Society》2004,34(3):327-336
The concept of miss distance-Euclidean, Mahalanobis, etc.-is a fundamental, far-reaching, and taken-for-granted element of the engineering theory and practice of single-target systems. In this paper we introduce a comprehensive L/sub p/-type theory of distance metrics for multitarget (and, more generally, multiobject) systems. We show that this theory extends, and provides a rigorous theoretical basis for, an intuitively appealing optimal-assignment approach proposed by Drummond for evaluating the performance of multitarget tracking algorithms. We describe tractable computational approaches for computing such metrics based on standard optimal assignment or convex optimization techniques. We describe the potentially far-reaching implications of these metrics for applications such as performance evaluation and sensor management. In the former case, we demonstrate the application of multitarget miss-distance metrics as measures of effectiveness (MoEs) for multitarget tracking algorithms. 相似文献
78.
Although word stress has been hailed as a powerful speech-segmentation cue, the results of 5 cross-modal fragment priming experiments revealed limitations to stress-based segmentation. Specifically, the stress pattern of auditory primes failed to have any effect on the lexical decision latencies to related visual targets. A determining factor was whether the onset of the prime was coarticulated with the preceding speech fragment. Uncoarticulated (i.e., concatenated) primes facilitated priming. Coarticulated ones did not. However, when the primes were presented in a background of noise, the pattern of results reversed, and a strong stress effect emerged: Stress-initial primes caused more pruning than non-initial-stress primes, regardless of the coarticulatory cues. The results underscore the role of coarticulation in the segmentation of clear speech and that of stress in impoverished listening conditions. More generally, they call for an integrated and signal-contingent approach to speech segmentation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
79.
80.
Katharina N?ske Hans-Jürgen Stark Leonard Nevaril Manuel Berning Lutz Langbein Ashish Goyal Sven Diederichs Petra Boukamp 《International journal of molecular sciences》2016,17(2)
Despite decades of skin research, regulation of proliferation and homeostasis in human epidermis is still insufficiently understood. To address the role of mitoses in tissue regulation, we utilized human long-term skin equivalents and systematically assessed mitoses during early epidermal development and long-term epidermal regeneration. We now demonstrate four different orientations: (1) horizontal, i.e., parallel to the basement membrane (BM) and suggestive of symmetric divisions; (2) oblique with an angle of 45°–70°; or (3) perpendicular, suggestive of asymmetric division. In addition, we demonstrate a fourth substantial fraction of suprabasal mitoses, many of which are committed to differentiation (Keratin K10-positive). As verified also for normal human skin, this spatial mitotic organization is part of the regulatory program of human epidermal tissue homeostasis. As a potential marker for asymmetric division, we investigated for Numb and found that it was evenly spread in almost all undifferentiated keratinocytes, but indeed asymmetrically distributed in some mitoses and particularly frequent under differentiation-repressing low-calcium conditions. Numb deletion (stable knockdown by CRISPR/Cas9), however, did not affect proliferation, neither in a three-day follow up study by life cell imaging nor during a 14-day culture period, suggesting that Numb is not essential for the general control of keratinocyte division. 相似文献