Pharmacological effects of BAM-1120, a novel ergoline derivative, on central dopaminergic functions in rats

Based on data gathered from patients, psychiatrists, and social workers at the Hawaii State Hospital, it was determined that the majority of patients had been in the hospital for more than one year, were committed for forensic reasons, and did not need continued hospitalization. An inter-agency systems approach is needed to address the issue of length of patient stay.     

Suppressive effects of the endothelin receptor (ETA) antagonist BQ-123 on ET-1-induced reduction of lipoprotein lipase activity in 3T3-L1 adipocytes

Endothelin (ET)-1 reduced heparin-releasable lipoprotein lipase (LPL) activity in 3T3-L1 adipocytes in a concentration-dependent manner. However, a selective ETB receptor agonist, [Ala1,3,11,15]ET-1, did not act like ET-1. The ET-1-induced decrease in LPL activity was suppressed by a selective ETA receptor antagonist, BQ-123: the concentration-response curve for the ET-1 reduction of LPL activity was shifted to the right in the presence of BQ-123 in a concentration-dependent manner. This antagonistic effect of BQ-123 clarifies that the ETA receptor is responsible for the ET-1-induced reduction of LPL activity in 3T3-L1 adipocytes, which suggests that there is therapeutic potential for ETA antagonists in LPL-related lipoprotein disorders.     

Effects of intracerebroventricular injection of histamine and its related compounds on rectal temperature in mice

Effects of intracerebroventricular injection of histamine and its related compounds on rectal temperature were studied in mice. Histamine (0.1-1.0 mu g) and histidine (500-1,000 mg/kg) caused a dose-related hypothermia. H1 agonist, 2-methylhistamine and 2-thiazolylethylamine also displayed a dose-dependent hypothermia. In addition, H2 agonists, 4-methylhistamine and dimaprit elicited a decrease in body temperature. Preinjection of not only H1-antagonists (diphenhydramine and chlorpheniramine) but also H2 antagonists (cimetidine and ranitidine) abolished histamine-induced hypothermia. Either intracerebroventricular or intraperitoneal injection of thioperamide, a histamine H3 antagonist, showed hypothermia. The hypothermic effect produced by intracerebroventricular injection of thioperamide was significantly blocked by (R)-alpha-methylhistamine, a selective H3 agonist. In addition, the effect induced by thioperamide was inhibited by H1 and H2 antagonists, indicating that the H3 receptor also participates in histamine-induced hypothermia.     

Microbiological quality of aseptic packaging and the effect of pin-holes on sterility of aseptic products

The microbiological quality of aseptic packaging (the board for long-life milk) was good and viable counts were under 50cfu per package. The percentage of defective samples that had viable microorganisms after 7–14 days of pre-incubation was 54% (one pin-hole) and 54% (ten pin-holes) for coffee beverage packed in board B and 17% (one pin-hole) and 17% (10 pin-holes) for milk packed in board A. The differences in the percentage of defective samples between milk and coffee beverage and between board A and board B were significant, but no difference was observed between one and ten pin-holes. An increase in fat content or a decrease in protein content increased the permeation of milk components through the pin-holes. The microorganisms isolated from the products with artificial pin-holes were f actobacillus sp. (52%), Streptococcus sp. (48 %) and Enterobacteriaceae (34%).     

Polyethylene glycol-modified bilirubin oxidase improves hepatic energy charge and urinary prostaglandin levels in rats with obstructive jaundice

BACKGROUNDS/AIMS: No study has so far been conducted to clarify whether the presence of hyperbilirubinemia is detrimental to liver and renal functions. In the present study, the effects of polyethylene glycol-modified bilirubin oxidase (PEG-BOX) therapy on liver and renal function tests, hepatic energy charge and urinary prostaglandin levels were evaluated in a rat model of obstructive jaundice. METHODS: Sprague-Dawley rats were used in the experimental model of obstructive jaundice. PEG-BOX or an equivalent amount of PEG alone was intravenously injected into the animals and sampling of blood and urine, and liver harvesting were done sequentially after bile duct ligation. RESULTS: Conventional liver function tests showed no difference between PEG-BOX and control groups. However, bilirubin concentrations in the peripheral blood and liver tissue specimens markedly decreased, and the hepatic energy charge significantly increased in the PEG-BOX group as compared to controls. The blood concentration of bile acid was lower, but its urinary excretion was higher in the PEG-BOX group than in the control group. In vitro incubation of PEG-BOX with serum from rats with obstructive jaundice decreased the concentration of bilirubin but not that of bile acid. The urinary levels of prostaglandin E2 and the thromboxane B2/6-keto-prostaglandin Fla ratio were significantly lower in the PEG-BOX group than in the control group. CONCLUSIONS: The systemic reduction of bilirubin concentration may contribute to normalization of the urinary levels of prostaglandins and thromboxane B2, to decrease in serum bile acid levels, and to improvement of the hepatic energy charge in obstructive jaundice. These findings suggest that preoperative improvement of jaundice may be beneficial to patients with obstructive jaundice.     

Plasma levels of interleukin-1 receptor antagonist (IL-1ra) and severity of illness in patients with burns

The present study was conducted to determine whether a plasma interleukin-1 receptor antagonist (IL-1ra) would reflect the severity of burn injury and to examine the relation between IL-1ra and the cytokines. We studied 24 burn patients in whom the total burn surface area (TBSA) accounted for at least 20% of the body surface, and in whom serial blood samples could be obtained beginning immediately after the burn injury. Plasma levels of IL-1ra were determined by enzyme-linked immunosorbent assay (ELISA). Plasma levels of tumor necrosis factor-alpha (TNF-alpha), IL-6, and IL-8 were also determined by ELISA. Endotoxin was measured by an endotoxin-specific synthetic substrate method. There was a significant correlation between the plasma levels of IL-1ra and TBSA during the first week following burn injury. The IL-1ra level was the highest immediately after the burn injury. The level decreased markedly thereafter, and again rose when infection occurred. The IL-1ra level was extraordinarily elevated in patients who developed concomitant sepsis, septic shock or the septic multiple organ dysfunction syndrome. The IL-1ra level on admission and the maximum IL-1ra level during the observation period were significantly higher in the patients who eventually died than in the survivors. There was a significant correlation between the level of IL-1ra and that of TNF-alpha, IL-6 or IL-8 during the observation period. No correlation was found between IL-1ra and endotoxin. The plasma IL-1ra level was closely correlated with the severity of inflammation and the clinical status of the burn patients, regardless of the infection. Results suggest that IL-1ra can serve as an index of the systemic inflammatory response syndrome (SIRS).     

Fracture of the tarsal navicular associated with calcaneonavicular coalition: a case report

Fracture of the navicular occurred in a 23-year-old male with unilateral cartilaginous calcaneonavicular coalition. Inversion sprain of the left foot caused the longitudinal navicular fracture next to the coalition. En bloc resection from the beak of the calcaneus to the fracture line of the navicular including the calcaneonavicular coalition was performed, and a short leg cast was applied for 10 days. Four weeks after surgery, he returned to his preinjury level of activity and job as a long-distance truck driver. Two years and 3 months after surgery, he was totally asymptomatic and had gained a full range of subtalar motion. He had no peroneal spasm or recurrence of the coalition.     

Measurements of effective dielectric permittivity of microstrip-line-type liquid crystal devices using inductive coupled ring resonator

The effective dielectric permittivity of microstrip-line-type liquid crystal devices was determined in the 3-33 GHz frequency range by using a newly developed inductive coupled ring resonator. The frequency performance of /spl epsiv/'/sub /spl par// and /spl epsiv/'/sub /spl perp// can be obtained from the measured and simulated resonant frequencies of an inductive coupled ring resonator with or without a DC electric field E/sub 0/.     

Timer-based composition of fault-containing self-stabilizing protocols

One of the desired properties of distributed systems is self-adaptability against faults. Self-stabilizing protocols provide autonomous recovery from any finite number of transient faults. However, in practice, catastrophic faults rarely occur, while small-scale faults are more likely to occur. Fault-containing self-stabilizing protocols promise not only self-stabilization but also containment of the effect of small-scale faults, i.e., they promise quick recovery and small effect for small-scale faults. Hierarchical composition of self-stabilizing protocols is expected to ease the design of new self-stabilizing protocols. However, existing composition techniques for self-stabilizing protocols cannot preserve the fault-containment property of source protocols. In this paper, we propose a novel timer-based hierarchical composition of fault-containing self-stabilizing protocols that preserves the fault-containment property of source protocols. To implement timers, we propose a local neighborhood synchronizer that synchronizes limited number of processes during a short time after a fault without involving the entire network into the synchronization. The proposed composition technique facilitates the design of new fault-containing self-stabilizing protocols and enhances the reusability of existing fault-containing self-stabilizing protocols.     

Observations on non-silent self-stabilizing algorithms in sensor networks with probabilistically intermittent link failures

A wireless sensor network is a set of nodes, each is equipped with a sensing device and a wireless communication device. Because centralized control is hard to achieve in a large scale sensor network, self-∗ is a key concept in the design of a wireless sensor network. Self-stabilization is one of the self-∗ properties, and it is one of the most promising theoretical backgrounds for self-organizing wireless sensor network protocols. Herman [T. Herman, Models of self-stabilization and sensor networks, in: Proceedings of the 5th International Workshop of Distributed Computing, IWDC, 2003, pp. 205-214] proposed Cached Sensornet Transform (CST for short) for design and implementation of self-stabilizing algorithms for sensor networks. It transforms a self-stabilizing algorithm in a high-level computational model to a program for sensor networks. Our contribution in this paper is threefold. We show that there exists a non-silent algorithm that behaves correctly in a high-level computational model but its transformed version by CST does not behave correctly if packets are lost. We show a sufficient condition for original algorithms and networks such that the algorithm transformed by CST behaves correctly. As a case study, we present a token circulation algorithm that behaves correctly by CST and derive the upper bound of its expected convergence time.