Measurement of DNA strand breakage and DNA repair induced with hydrogen peroxide using single cell gel electrophoresis, alkaline DNA unwinding and alkaline elution of DNA

Three techniques: single cell gel electrophoresis (SCGE), alkaline elution of DNA (AE), and alkaline DNA unwinding (ADU) were chosen to compare the sensitivity among these methods in detection of DNA damage and repair in human diploid VH10 cell line after short-term exposure to hydrogen peroxide. Using SCGE technique a dose-dependent increase in DNA migration was found in cells exposed to hydrogen peroxide in concentration range from 10 micromol/l to 100 micromol/l. Alkaline DNA unwinding method detected increased level of single strand breaks (ssb) in concentration range from 25 micromol/l to 100 micromol/l of H2O2, and alkaline elution of DNA estimated increased DNA elution rate from concentration 50 micromol/l of H2O2. In a time course study to evaluate the kinetics of DNA repair, both SCGE and ADU techniques showed that the repair of DNA strand breaks is very rapid; the level of ssb in treated cells has returned to near the background level within two hours. After this time damage remaining in the DNA was in the form of oxidised bases as revealed the incubation of treated cells with specific DNA repair endonuclease, formamidopyrimidine-DNA glycosylase.     

Exchange rates at the lipid-protein interface of the myelin proteolipid protein determined by saturation transfer electron spin resonance and continuous wave saturation studies

The microwave saturation properties of various spin-labeled lipids in reconstituted complexes of the myelin proteolipid protein with dimyristoyl phosphatidylcholine have been studied both by conventional and saturation transfer electron spin resonance (ESR) spectroscopy. In the fluid phase, the conventional ESR spectra consist of a fluid and a motionally restricted (i.e., protein-associated) component, whose relative proportions can be determined by spectral subtractions and depend on the selectivity of the particular spin-labeled lipid for the protein. At 4 degrees C when the bulk lipid is in the gel phase, the integrated intensity of the saturation transfer ESR spectra displays a linear dependence on the fraction of motionally restricted lipid that is deduced from the conventional ESR spectra in the fluid phase, indicating the presence of distinct populations of free and protein-interacting lipid with no exchange between them on the saturation transfer ESR time scale in the gel phase. At 30 degrees C when the bulk lipid is in the fluid phase, the saturation transfer integral displays a nonlinear dependence on the fraction of motionally restricted lipid, consistent with exchange between the two lipid populations on the saturation transfer ESR time scale in the fluid phase. For lipid spin labels with different selectivities for the protein in complexes of fixed lipid/protein ratio, the data in the fluid phase are consistent with a constant (diffusion-controlled) on-rate for exchange at the lipid-protein interface. Values ranging between 1 and 9 x 10(6) s-1 are estimated for the intrinsic off-rates for exchange of spin-labeled stearic acid and phosphatidylcholine, respectively, at 30 degrees C. Conventional continuous wave saturation experiments lead to similar conclusions regarding the lipid exchange rates in the fluid and gel phases of the lipid/protein recombinants. The ESR saturation studies therefore demonstrate exchange on the time scale of the nitroxide spin-lattice relaxation at the lipid-protein interface of myelin proteolipid/dimyristoyl phosphatidylcholine complexes in the fluid phase but not in the gel phase.     

Evaluation of hemodynamic parameters in patients with right ventricular infarction during rehabilitation

Hemodynamic data in 43 patients with right and left ventricular infarction and 39 with left ventricular infarction were compared. Right atrial pressure, pulmonary capillary wedge pressure, mean pulmonary artery pressure, left ventricular end-diastolic pressure before and after ventricular angiogram, cardiac index, left ventricular stroke volume index, right ventricular stroke work index were evaluated, as well as ratios of right atrial and pulmonary capillary wedge pressure, right and left ventricular end-diastolic pressure, right ventricular end-diastolic and pulmonary capillary wedge pressure, left ventricular ejection fraction calculated from left ventricular angiogram, Berentey's score, and cardiac volume index. Using ONEWAY analysis there was no significant difference between the two groups in period of rehabilitation. In 15 patients with right ventricular infarction regression of ECG changes was observed in lead V3R also without significant influence on hemodynamic data.     

Anomalous self-diffusion in a single crystal of α-zirconium

Self-diffusion in an α-zirconium single crystal was investigated over the temperature range 779 to 1128 K. The measurements were made by simultaneous diffusion of ⁹⁵Zr and its radioactive daughter ⁹⁵Nb and by subsequent ion-beam-sputter sectioning (IBS). The activity of ⁹⁵Zr in each section was determined from the 724 keV γ peak using a Ge-Li spectrometer. Gaussian penetration curves were observed. The diffusivities vary in the range between $\text{3.28 × 10}{}^{\mathrm{?23}}\text{m}{}^2\text{s}{}^{\mathrm{?1}}$ and $\text{1.04 × 10}{}^{\mathrm{?18}}\text{m}{}^2\text{s}{}^{\mathrm{?1}}$. The present results are compared with previous studies of self-diffusion in α- and β-Zr. The Arrhenius plot of self-diffusion coefficients in α-Zr is strongly curved downward. This means that an anomalous behaviour of self-diffusion is observed not only in the β- but also in the α-phase of Zr. Some results on the diffusion of ⁹⁵Nb in α-Zr are reported in an Appendix.     

Implementation of QA and QC standards in radiology in Slovakia

The Slovak government organizes the radiation protection policy through its regulatory authorities within the Ministry of Health, on a central level. The health protection regulations are compatible with international standards and recommendations of the ICRP and the EC. The general requirements on quality assurance (QA) and quality control (QC) (acceptance, constancy and routine tests), the guidance levels for various types of radiological examinations and the instructions for optimisation procedures are supported by Slovak technical standards, compatible with European standards. But the QA/QC process, as well as the training of the staff, needs improvement. The Slovak Medical University participates in the QA implementation through organising and managing national audits for control of the QA programme. In this paper, we present results of patient dose measurement studies carried out in the Slovak Republic, in the framework of activities of the Slovak Commission of QA established by the Slovak Ministry of Health.     

Incorporation of single-wall carbon nanotubes into an organic polymer monolithic stationary phase for mu-HPLC and capillary electrochromatography

Single-wall carbon nanotubes (SWNT) were incorporated into an organic polymer monolith containing vinylbenzyl chloride (VBC) and ethylene dimethacrylate (EDMA) to form a novel monolithic stationary phase for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC). The retention behavior of neutral compounds on this poly(VBC-EDMA-SWNT) monolith was examined by separating a mixture of small organic molecules using micro-HPLC. The result indicated that incorporation of SWNT enhanced chromatographic retention of small neutral molecules in reversed-phase HPLC presumably because of their strongly hydrophobic characteristics. The stationary phase was formed inside a fused-silica capillary whose lumen was coated with covalently bound polyethyleneimine (PEI). The annular electroosmotic flow (EOF) generated by the PEI coating allowed peptide separation by CEC in the counterdirectional mode. Comparison of peptide separations on poly(VBC-EDMA-SWNT) and on poly(VBC-EDMA) with annular EOF generation revealed that the incorporation of SWNT into the monolithic stationary phase improved peak efficiency and influenced chromatographic retention. The structures of pretreated SWNT and poly(VBC-EDMA-SWNT) monolith were examined by high-resolution transmission electron microscopy, Raman spectroscopy, scanning electron microscopy, and multipoint BET nitrogen adsorption/desorption.     

Structure-activity relationship studies of novel somatostatin analogs with antitumor activity

A series of new somatostatin analogs were synthesized in order to study the relative importance of specific substitutions in relation to selectivity between their endocrine and antitumor effects. Substitutions were carried out in all positions, except for Lys in position 5. Peptides were tested for their ability to inhibit in vitro and in vivo GH release, proliferation of the MCF 7 breast carcinoma cell line and tyrosine kinase activity in the HT 29 human colon carcinoma cell line. Selective biological activity was achieved in GH release and antitumor activity by the different amino acid substitutions. One of the analogs, with a five-residue ring (D-Phe-Cys-Tyr-D-Trp-Lys-Cys-Thr-NH2, TT-232), was unique. It had no GH release inhibitory activity, but did have strong tyrosine kinase inhibitory and antiproliferative effects.